Publications by authors named "Benedetta Canalis"
Article Synopsis
- RNA-seq is the leading method for identifying gene fusions in non-small cell lung cancer (NSCLC), but managing RNA stability in tissue samples presents challenges that can lead to test failures.
- The study analyzed 140 NSCLC tumor samples and found that factors like higher RNA concentration and lower fragmentation improve RNA-seq success rates.
- Smaller biopsies and freshly stored samples (under 30 days) yield better results, allowing for improved planning in molecular diagnostics for NSCLC.
Background: germline monoallelic variants have been detected in a number of patients affected by breast/ovarian cancer or endometrial cancer, suggesting a potential susceptibility role, though their significance remains elusive since the disease mechanism is normally recessive. Hence, the aim of this research was to explore the hypothesis that a second hit could have arisen in the other allele in the tumor tissue.
Methods: we used Sanger sequencing and immunohistochemistry to search for a second variant in the tumoral DNA and to assess protein expression, respectively.