The homodimerization domain of the Stl repressor is crucial for efficient inhibition of mycobacterial dUTPase.

The dUTPase is a key DNA repair enzyme in Mycobacterium tuberculosis, and it may serve as a novel promising anti-tuberculosis target. Stl repressor from Staphylococcus aureus was shown to bind to and inhibit dUTPases from various sources, and its expression in mycobacterial cells interfered with cell growth. To fine-tune and optimize Stl-induced inhibition of mycobacterial dUTPase, we aimed to decipher the molecular details of this interaction.

Revising cancer incidence in a Central European country: a Hungarian nationwide study between 2011-2019 based on a health insurance fund database.

Background: The nationwide HUN-CANCER EPI study examined cancer incidence and mortality rates in Hungary from 2011 to 2019.

Methods: Using data from the National Health Insurance Fund (NHIF) and Hungarian Central Statistical Office (HCSO), our retrospective study analyzed newly diagnosed malignancies between Jan 1, 2011, and Dec 31, 2019. Age-standardized incidence and mortality rates were calculated for all and for different tumor types using both the 1976 and 2013 European Standard Populations (ESP).

Background parenchymal enhancement on contrast-enhanced mammography: associations with breast density and patient's characteristics.

Purpose: To evaluate if background parenchymal enhancement (BPE) on contrast-enhanced mammography (CEM), graded according to the 2022 CEM-dedicated Breast Imaging Reporting and Data System (BI-RADS) lexicon, is associated with breast density, menopausal status, and age.

Methods: This bicentric retrospective analysis included CEM examinations performed for the work-up of suspicious mammographic findings. Three readers independently and blindly evaluated BPE on recombined CEM images and breast density on low-energy CEM images.

Comparing accuracy of tomosynthesis plus digital mammography or synthetic 2D mammography in breast cancer screening: baseline results of the MAITA RCT consortium.

Aim: The analyses here reported aim to compare the screening performance of digital tomosynthesis (DBT) versus mammography (DM).

Methods: MAITA is a consortium of four Italian trials, REtomo, Proteus, Impeto, and MAITA trial. The trials adopted a two-arm randomised design comparing DBT plus DM (REtomo and Proteus) or synthetic-2D (Impeto and MAITA trial) versus DM; multiple vendors were included.

Effects of Season, Habitat, and Host Characteristics on Ectoparasites of Wild Rodents in a Mosaic Rural Landscape.

Despite the large number of studies on rodent ectoparasites-most of them vectors of epidemiologically important pathogens-infestation patterns remain poorly understood in various ecological contexts, such as the highly patchy agricultural landscapes. We aimed to relate the infestation of rodents to temporal, habitat, and host variables. We assessed the difference in parasite prevalence and mean abundance depending on host sex, age, and body weight, season, and land use intensity.

Geographical and environmental patterns of Carpathian land snail faunas in a region of high endemicity.

The land snail faunas of limestone gorges of Romanian Carpathians were sampled to test the effect of geographic and environmental factors on the malacofauna richness and composition. A total of 134 sites within 28 limestone gorges were surveyed during 2011-2019 using a combined strategy of visual search and litter/topsoil analysis. Environmental variables such as geographic location, altitude, climate, microhabitat type, dominant vegetation, tree cover and width of the gorge were recorded to detect the relationship with species richness and composition.

Opposite trends in incidence of breast cancer in young and old female cohorts in Hungary and the impact of the Covid-19 pandemic: a nationwide study between 2011-2020.

Background: This nationwide study examined breast cancer (BC) incidence and mortality rates in Hungary between 2011-2019, and the impact of the Covid-19 pandemic on the incidence and mortality rates in 2020 using the databases of the National Health Insurance Fund (NHIF) and Central Statistical Office (CSO) of Hungary.

Methods: Our nationwide, retrospective study included patients who were newly diagnosed with breast cancer (International Codes of Diseases ICD)-10 C50) between Jan 1, 2011 and Dec 31, 2020. Age-standardized incidence and mortality rates (ASRs) were calculated using European Standard Populations (ESP).

Taxonomic and functional diversity of land snails reflects habitat complexity in riparian forests.

Habitat complexity affects the structure and dynamics of ecological communities, more often with increased complexity leading to greater species diversity and abundance. Among the terrestrial invertebrate groups, the low vagility of land snails makes them susceptible to react to small-scale habitat alteration. In the current paper we aimed to assess the relationship between taxonomic and functional composition and diversity of land snail communities and habitat structure in the riparian forest habitat.

-induced acute liver injury: A case report.

Drug-induced liver injury became one of the most important liver disorders and diagnostic challenge for clinicians and pathologists. Here, we report a rare case of -induced acute liver injury. The patient developed jaundice 2 weeks after starting intake and recovered within 5 months after withdrawal without any specific treatment.

Significant improvement in melanoma survival over the last decade: A Hungarian nationwide study between 2011 and 2019.

Background: Recent real-world studies have reported significant improvements in the survival of malignant melanoma in the past few years, mainly as a result of modern therapies. However, long-term survival data from Central Eastern European countries such as Hungary are currently lacking.

Methods: This nationwide, retrospective study examined melanoma survival in Hungary between 2011-2019 using the databases of the National Health Insurance Fund (NHIF) and Central Statistical Office (CSO) of Hungary.

Disentangling structural and functional responses of native versus alien communities by canonical ordination analyses and variation partitioning with multiple matrices.

Freshwaters are under accelerated human pressure, and mollusk communities are among its most sensitive, threatened, and valuable components. To the best of our knowledge, the overall effects of damming, environment, space, time, and invasive alien mollusk species, on structural and functional responses of native mollusk communities were not yet compared. Using historical information and recent data from a river, we aimed to investigate and disentangle these effects and evaluate the differences in structural and functional responses of natives and alien invasives to the same predictors.

Contrast-enhanced mammography for the assessment of screening recalls: a two-centre study.

Objectives: To evaluate the potential of contrast-enhanced mammography (CEM) for reducing the biopsy rate of screening recalls.

Methods: Recalled women were prospectively enrolled to undergo CEM alongside standard assessment (SA) through additional views, tomosynthesis, and/or ultrasound. Exclusion criteria were symptoms, implants, allergy to contrast agents, renal failure, and pregnancy.

Ancient genomes reveal origin and rapid trans-Eurasian migration of 7 century Avar elites.

The Avars settled the Carpathian Basin in 567/68 CE, establishing an empire lasting over 200 years. Who they were and where they came from is highly debated. Contemporaries have disagreed about whether they were, as they claimed, the direct successors of the Mongolian Steppe Rouran empire that was destroyed by the Turks in ∼550 CE.

Robust Recombinant Expression of Human Placental Ribonuclease Inhibitor in Insect Cells.

Ribonuclease inhibitors (RIs) are an indispensable biotechnological tool for the detection and manipulation of RNA. Nowadays, due to the outbreak of COVID-19, highly sensitive detection of RNA has become more important than ever. Although the recombinant expression of RNase inhibitors is possible in , the robust expression is complicated by maintaining the redox potential and solubility by various expression tags.

Improving Quality Indicator of Melanoma Management - Change of Melanoma Mortality-to-Incidence Rate Ratio Based on a Hungarian Nationwide Retrospective Study.

Introduction: The incidence of melanoma has been increasing in the last decades. A retrospective Hungarian epidemiological study provided real-world data on incidence and mortality rates. There have been changing trends in incidence in Hungary in the last decade and mortality decreased, shifting mortality-to-incidence rate ratios (MIR).

MRI versus CT in the detection of brain lesions in patients with infective endocarditis before or after cardiac surgery.

Purpose: Imaging of brain involvement in infective endocarditis can drive the clinical management of this serious condition. MRI is very sensitive, but CT is more readily available. In this retrospective study, we compared the detection rates of CT and MRI.

Variation partitioning in double-constrained multivariate analyses: linking communities, environment, space, functional traits, and ecological niches.

Constrained multivariate analysis is a common tool for linking ecological communities to environment. The follow-up is the development of the double-constrained correspondence analysis (dc-CA), integrating traits as species-related predictors. Further, methods have been proposed to integrate information on phylogenetic relationships and space variability.

Responses of small mammals to habitat characteristics in Southern Carpathian forests.

Compared to Northern Carpathians, the small mammal fauna of Southern Carpathian forests is poorly known, with no data on habitat use; our study seeks to fill this gap. To this end, we conducted a survey in the Southern Carpathians for five years, assessing habitat use by small mammals in forests along an elevational gradient. Trapping was done using live traps set in transects at elevations between 820 and 2040 m.

Modulated Electro-Hyperthermia Facilitates NK-Cell Infiltration and Growth Arrest of Human A2058 Melanoma in a Xenograft Model.

Modulated electro-hyperthermia (mEHT), induced by 13.56 MHz radiofrequency, has been demonstrated both in preclinical and clinical studies to efficiently induce tumor damage and complement other treatment modalities. Here, we used a mouse xenograft model of human melanoma (A2058) to test mEHT (~42°C) both alone and combined with NK-cell immunotherapy.

[The adjuvant killing effect of modulated electro-hyperthermia combined with chemotherapy on B16F10 melanoma cells].

Our aim was to detect the effect of modulated electro-hyperthermia (mEHT) on cell viability and to examine if hyperthermia can augment the cell killing effect of various chemotherapeutic agents. B16F10 melanoma cells were treated for 30, 60, 90 and 120 minutes with mEHT using LabEHY100 (OncothermTM). Cell viability was measured using MTT assay and apoptosis by annexin V/7-AAD staining using flow cytometry 24 hours post-treatment.

Changing Trends in Melanoma Incidence and Decreasing Melanoma Mortality in Hungary Between 2011 and 2019: A Nationwide Epidemiological Study.

Background: The incidence of malignant melanoma has continually increased during the past few decades, however, certain reports suggest a recent change in trends. The aim of our study was to examine the epidemiology of melanoma in Hungary.

Methods: This nationwide, retrospective, longitudinal study included melanoma patients diagnosed between 1 January 2009 and 31 December 2019 using the databases of the National Health Insurance Fund (NHIF) and Central Statistical Office (CSO) of Hungary.

Suppression of Metastatic Melanoma Growth in Lung by Modulated Electro-Hyperthermia Monitored by a Minimally Invasive Heat Stress Testing Approach in Mice.

Modulated electro-hyperthermia (mEHT) is a novel complementary therapy in oncology which is based on the higher conductivity and permittivity of cancerous tissues due to their enhanced glycolytic activity and ionic content compared to healthy normal tissues. We aimed to evaluate the potential of mEHT, inducing local hyperthermia, in the treatment of pulmonary metastatic melanoma. Our primary objective was the optimization of mEHT for targeted lung treatment as well as to identify the mechanism of its potential anti-tumor effect in the B16F10 mouse melanoma pulmonary metastases model while investigating the potential treatment-related side effects of mEHT on normal lung tissue.

[Preclinical and clinical investigation and development of electromagnetic oncological device - experience with solid tumors].

Our objective was to develop an electromagnetic tumor therapy device in a consortial cooperation between Semmelweis University and Oncotherm Ltd., to provide data and contribute to the development of the next generation of devices through preclinical, clinical and developmental modules via in vivo, in vitro studies, and patient treatments. Our numerous preclinical studies support the efficacy of mEHT.

[Vaccine hesitancy among primary healthcare professionals in Hungary].

The attitude to immunization and the issue of vaccine hesitancy in health care workers (HCWs) have been studied in a former survey performed by ECDC (European Centre for Disease Prevention and Control). Our aim was to study the immunization attitude of primary care paediatricians, general practitioners and primary care nurses in Hungary. : We studied vaccine hesitancy in HCWs by way of a questionnaire, developed on the basis of a recent similar survey by ECDC in four countries.

Stress-Induced, p53-Mediated Tumor Growth Inhibition of Melanoma by Modulated Electrohyperthermia in Mouse Models without Major Immunogenic Effects.

Modulated electrohyperthermia (mEHT), an innovative complementary technique of radio-, chemo-, and targeted oncotherapy modalities, can induce tumor apoptosis and contribute to a secondary immune-mediated cancer death. Here, we tested the efficiency of high-fever range (~42 °C) mEHT on B16F10 melanoma both in cell culture and allograft models. In vivo, mEHT treatment resulted in significant tumor size reduction when repeated three times, and induced major stress response as indicated by upregulated cytoplasmic and cell membrane hsp70 levels.