Manganese Superoxide Dismutase Gene-Modified Mesenchymal Stem Cells Attenuate Acute Radiation-Induced Lung Injury.

Radiation-induced lung injury (RILI) is a major clinical complication for radiotherapy in thoracic tumors. An immediate effect of lung irradiation is the generation of reactive oxygen that can produce oxidative damage to DNA, lipids, and proteins resulting in lung cell injury or death. Currently, the medical management of RILI remains supportive.

Application of human bone marrow-derived mesenchymal stem cells in the treatment of radiation-induced Gastrointestinal syndrome.

Nuclear accidents and terrorism present a serious threat for mass casualty. Accidental or intended radiation exposure leads to radiation-induced gastrointestinal (GI) syndrome. However, currently there are no approved medical countermeasures for GI syndrome.

Classification, clinicopathologic features and treatment of gastric neuroendocrine tumors.

Gastric neuroendocrine tumors (GNETs) are rare lesions characterized by hypergastrinemia that arise from enterochromaffin-like cells of the stomach. GNETs consist of a heterogeneous group of neoplasms comprising tumor types of varying pathogenesis, histomorphologic characteristics, and biological behavior. A classification system has been proposed that distinguishes four types of GNETs; the clinicopathological features of the tumor, its prognosis, and the patient's survival strictly depend on this classification.

[Expression of Foxa2 and its early alarm value of cancerous in gastric polyps].

Objective: To explore the expression of Foxa2 in different pathological types of gastric polyps and examine the correlation with cancerous risk.

Methods: According to computerize random number, a total of 2000 patients were selected to receive endoscopic biopsy during November 2011 to October 2012. Tissues were harvested from 170 with gastric polyps and suspicious cancerous lesions and their histological types detected.

Manganese superoxide dismutase gene therapy protects against irradiation- induced intestinal injury.

Radiation-induced intestinal injury is a common complication in radiotherapy for solid organ malignancies in abdomen or pelvis. However, currently there are no approved medical countermeasures for radiation-induced intestinal injury. Therefore, it is urgent to develop new treatments for radiation-induced intestinal injury.

[Oxymatrine could promote mesenchymal stem cell therapy in hepatic fibrosis rats: an experimental research].

Objective: To investigate whether oxymatrine (OM) could promote mesenchymal stem cell (MSC) therapy in CCl4-induced hepatic fibrosis (HF) in rats and to initially explore its mechanisms.

Methods: Totally 50 male SD rats were randomly divided into five groups,i.e.

[Differential proteomic analysis of rat hepatic stellate cells treated by oxymatrine liposomes using two-dimensional electrophoresis].

Objective: To analyze differentially expressed proteins of hepatic stellate cells (HSCs) treated with oxymatrine (OMT) liposomes, thus further exploring the molecular mechanism of OMT liposomes for treating liver fibrosis.

Methods: A rat model of CCl4 induced chronic liver fibrosis was established. HSCs were perfusion isolated from modeled SD rats and cultured in vitro .

Suppression of CB1 cannabinoid receptor by lentivirus mediated small interfering RNA ameliorates hepatic fibrosis in rats.

It is recognized that endogenous cannabinoids, which signal through CB1 receptors in hepatic stellate cells (HSCs), exert a profibrotic effect on chronic liver diseases. In this study, we suppressed CB1 expression by lentivirus mediated small interfering RNA (CB1-RNAi-LV) and investigated its effect on hepatic fibrosis in vitro and in vivo. Our results demonstrated that CB1-RNAi-LV significantly inhibited CB1 expression, and suppressed proliferation and extracellular matrix production in HSCs.

[The value of serum intestinal fatty acid binding protein measurement in discriminating intestinal ischemia in patients with acute abdomen].

Objective: To assess the differential diagnostic value of serum intestinal fatty acid binding protein (I-FABP) in distinguishing intestinal ischemia patients from acute abdomen patients.

Methods: A total of 151 patients with acute abdomen and 17 healthy controls from the PLA General Hospital were enrolled from November, 2009 to August, 2011. Serum I-FABP levels were measured by ELISA.

Oxymatrine liposome attenuates hepatic fibrosis via targeting hepatic stellate cells.

Aim: To investigate the potential mechanism of Arg-Gly-Asp (RGD) peptide-labeled liposome loading oxymatrine (OM) therapy in CCl₄-induced hepatic fibrosis in rats.

Methods: We constructed a rat model of CCl₄-induced hepatic fibrosis and treated the rats with different formulations of OM. To evaluate the antifibrotic effect of OM, we detected levels of alkaline phosphatase, hepatic histopathology (hematoxylin and eosin stain and Masson staining) and fibrosis-related gene expression of matrix metallopeptidase (MMP)-2, tissue inhibitor of metalloproteinase (TIMP)-1 as well as type I procollagen via quantitative real-time polymerase chain reaction.

In vivo transplantation of bone marrow mesenchymal stem cells accelerates repair of injured gastric mucosa in rats.

Background: Adult stem cells provide a promising alternative for the treatment of injured tissues. We aimed to investigate the effect of in vivo transplantation of bone marrow mesenchymal stem cells (BMMSCs) on injured gastric mucosa in rats.

Methods: The gastric ulcer in rats was induced by indomethacin.

Magnifying endoscopy in upper gastroenterology for assessing lesions before completing endoscopic removal.

Any prognosis of gastrointestinal (GI) cancer is closely related to the stage of the disease at diagnosis. Endoscopic submucosal dissection (ESD) and en bloc endoscopic mucosal resection (EMR) have been performed as curative treatments for many early-stage GI lesions in recent years. The technologies have been widely accepted in many Asian countries because they are minimally invasive and supply thorough histopathologic evaluation of the specimens.

[Preparation and identification of monoclonal antibody against human GCRG213].

Aim: To prepare and characterize the monoclonal antibody against human GCRG213.

Methods: The HIS-GCRG213 fusion protein was expressed in E.coli.

[A retrospective analysis of clinical characteristics and mortality of gastrointestinal bleeding in 414 elderly patients].

Objective: To analyze clinical characteristics of patients with gastrointestinal bleeding (GIB) and the death-related risk factors.

Methods: A retrospective analysis was conducted in 414 patients hospitalized for GIB during a 16-year period of 1994 to 2009. Logistic regression analysis identified predictors of mortality.

Integrin alphavbeta6 promotes tumor tolerance in colorectal cancer.

Tumor immune tolerance plays a critical role in tumor cell survival; the establishment of tumor immune tolerance is incompletely understood yet. Integrin alphavbeta6 (avb6) is involved in tumor growth and metastasis. This study aimed to observe the effect of avb6 on the development of tumor tolerance in colorectal cancer (CRC).

[Factors for the recurrence after endoscopic resection of early gastric cancer].

Objective: To investigate the related factors of recurrence of early gastric cancer (EGC) after endoscopic resection.

Methods: Clinicopathologic data of 169 patients with EGC who underwent endoscopic resection and periodically followed up by the Chinese PLA General hospital were analyzed retrospectively.

Results: During a follow-up of 13 - 57 months (median time 24.

Th17 cells are increased with severity of liver inflammation in patients with chronic hepatitis C.

Background And Aim: As a newly identified subset of T helper cells, T-helper 17 cells (Th17) are major mediators of inflammation-associated disease. Some reports have revealed significantly increased Th17 cells in hepatitis B virus-infected patients, and a recent study has demonstrated that hepatitis C virus (HCV)-specific Th17 cells can be induced in vitro and regulated by transforming growth factor-β. This study attempted to characterize the role of Th17 cells in the disease progression of chronic hepatitis C (CHC).

Immunoglobulin kappa and immunoglobulin lambda are required for expression of the anti-apoptotic molecule Bcl-xL in human colorectal cancer tissue.

Objective: Aberrant expression of immunoglobulin (Ig) by cancer cells has been documented in a number of malignant tumors but its biological significance is unclear. Cancer cells overexpress anti-apoptotic molecules such as Bcl-xL. The present study aimed to examine the role of expression of Ig light-chain Igk and Iglambda in maintaining the high levels of Bcl-xL in colorectal cancer cells.

[Preparation, identification and preliminary application of the polyclonal antibody against gastric cancer-related protein GCRG224].

Aim: To prepare the polyclonal antibody against gastric cancer-related protein GCRG224.

Methods: The thioredoxin/GCRG224 fusion protein was expressed in E.coli.

[Expression of S100A6 in primary and metastatic human gastric cancer].

Objective: Some members of the S100 gene family have been suggested to be associated with cancer development and metastasis. Our previous cDNA micro-array studies have showed S100A6 expression is elevated in gastric cancer compared with that in paired normal mucosa. To validate our previous results and further investigate the possible role of S100A6 gene in gastric cancer, we carried out this detailed S100A6 expression analysis in more matched gastric cancer samples.

[Gene expression profile of human normal gastrointestinal tract tissues: bioinformatic study].

Aim: To identify up-regulated genes specific to human normal gastrointestinal tissues.

Methods: Study was made on human normal tissue gene expression database open to the public. Tissue-specific genes were identified using one-tailed student T test.

LINE-1 family member GCRG123 gene is up-regulated in human gastric signet-ring cell carcinoma.

Aim: To analyze the expression profiles of a human gastric-cancer-related gene, GCRG123, in human gastric signet-ring cell carcinoma tissues, and to perform bioinformatics analysis on GCRG123.

Methods: In situ hybridization was used to explore the GCRG123 expression pattern in paraffin-embedded gastric tissues, including 15 cases of signet-ring cell carcinoma, 15 of intestinal-type adenocarcinoma, and 15 of normal gastric mucosa. Northern blotting was used to analyze the differences in GCRG123 expression between stomach signet-ring cell carcinoma and intestinal-type adenocarcinoma tissues.

[Construction of a gastric cancer related gene GCRG213-specific siRNA expression vector and its effect on gastric cancer cells in vitro].

Objective: To investigate the effect of gene GCRG213 siRNA transfection into gastric cancer cell line MKN45 cells.

Methods: Two pairs of DNA sequences containing small hairpin structure to GCRG213 were designed and synthesized. The complement form was obtained by annealing and inserted into RNAi expression vector IMG-800.

[Clinical analysis of 38 elderly patients with early double primary cancers].

Objective: To study the clinical features and proper treatment of 38 elderly patients with early double primary cancers.

Methods: Thirty-eight elderly patients with early double primary cancers treated from January 1980 to March 2003 were retrospectively reviewed for involved organs, treatment and prognosis.

Results: Digestive tract was the most frequently involved, followed by urogenital system and lung.

[Preparation and characterization of rabbit antibody against gastric cancer-related protein GCRG213].

Aim: To prepare the rabbit antibody against gastric cancer-related protein GCRG213.

Methods: The thioredoxin/GCRG213 fusion protein was expressed in E. coli.