Manipulating volatile emission in tobacco leaves by expressing Aspergillus nigerbeta-glucosidase in different subcellular compartments.

Expression of the Aspergillus nigerbeta-glucosidase gene, BGL1, in Nicotiana tabacum plants (cv. Xanthi) had a profound effect on the volatile emissions of intact and crushed leaves. BGL1 was expressed under the control of the cauliflower mosaic virus (CaMV) 35S promoter and targeted to the cytoplasm, cell wall, lytic vacuole (LV), chloroplast or endoplasmic reticulum (ER).

Rapid liquid chromatography-ultraviolet determination of organic acids and phenolic compounds in red wine and must.

A reversed-phase liquid chromatography-ultraviolet (LC-UV) method is proposed for the rapid simultaneous analysis of the main carboxylic acids and polyphenols in must and wine. Good resolution was obtained for citric, tartaric, malic, lactic, acetic, caffeic, ellagic and gallic acids, (-)-epicatechin, quercetin and resveratrol. A novel silica-based column containing ether-linked phenyl groups, with polar end-capping and suitable for low-pH aqueous mobile phases was used and found to be superior to others tested.

Hydrolysis of glycosidically bound volatiles from apple leaves (Cv. Anna) by Aspergillus niger beta-glucosidase affects the behavior of codling moth (Cydia pomonella L.).

Glycosidically bound volatiles released from apple leaf extracts (cv. Anna) were analyzed by solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) and their behavioral effects on codling moth (CM) adults were evaluated in cage bioassays. The levels of 1-octanol, linalool, geraniol, benzyl alcohol, methyl salicylate, (2R,5R)-theaspirane, and (2S,5R)-theaspirane were significantly increased in the leaf extracts containing the Aspergillus niger beta-glucosidase (BGL1) compared to the extracts containing the glucoimidazole.

Fluorescent screening of transgenic Arabidopsis seeds without germination.

In this paper, we describe a reliable method for the screening and selection of Arabidopsis transgenic seeds within minutes without germination. Expression of the Aspergillus niger beta-glucosidase gene BGL1 in the plant's endoplasmic reticulum was used as a visual marker, together with 4-methylumbelliferyl-beta-D-glucopyranoside (MUGluc) as a substrate. Subsequent to incubation in a solution of MUGluc at room temperature for 2 to 15 min, transgenic seeds expressing BGL1 demonstrated a distinct fluorescent signal under UV light.