Triple-S Lift for Facial Rejuvenation.

Background: Since the beginning of the last century, when the aesthetic rejuvenating surgery was first described, there have been great changes in tactics and methods for solving the problems of age-related facial changes. Since the first description of the superficial muscular aponeurotic system (SMAS), there have been two main approaches to influencing this structure. These are various types of SMAS plications and different in-depth and technique sub-SMAS liftings.

[Studying the regression pattern of stage II and III retinopathy of prematurity by means of morphometric analysis of retinal vessels in different terms after laser retinal photocoagulation].

Dynamic evaluation of the diameters of central fundus vessels, 2nd order and peripheral vessels as well as the tortuosity index of central zone arteries in stage II and III retinopathy of prematurity (ROP), which showed regression after laser treatment, was performed. Obtained data can be used for estimation of the optimal follow-up period ensuring early detection of high probability for ROP progression after laser coagulation and decrease of the number of diagnostic examinations for patients whose pathologic process is resolving.

[Retinal vessels in retinopathy of prematurity].

Purpose: To define diagnostic morphometric parameters of central fundus and peripheral retinal vessels that characterize different types of retinopathy of prematurity (ROP) course.

Material And Methods: A total of 155 premature infants (310 eyes) with stage I, II, and III ROP were assessed as they were undergoing treatment in the Kaluga branch of "Eye Microsurgery" complex during 2010-2012. Patients with the same stage of the disease were divided into two groups according to the type of ROP course (type 1--ROP with low risk of progression, type 2--ROP with high risk of progression).

[Intraocular electrochemical lysis at the stage of endoresection of uveal melanoma (clinical experimental study)].

Introduction of novel techniques of tumor destruction allows endoresection to be a perspective eye-preserving method of uveal melanoma treatment. Further investigations are essential to assess endoresection efficacy.

[Prevalence of glucosyl transferase Lgt among Legionella pneumophila strains isolated from various sources].

Aim: Determine various members of Lgt glucosyl transferase family among microorganisms of Legionellaceae genus from museum collection and legionellae strains recently isolated in the Russian Federation and Germany.

Materials And Methods: Presence of 3 types of glucosyl transferase were determined in 73 strains of L. pneumophila and Legionella spp.

[Pathogenicity factors of bacteria with glycosylating activity].

A and B toxins of Clostridium difficile, a-toxin of C. novyi, lehal toxin of C. sordellii, and TpeL toxin of C.

[Pattern retinal laser coagulation in treatment of aggressive posterior retinopathy of prematurity].

Efficacy of pattern scanning laser coagulation in treatment of aggressive posterior retinopathy of prematurity (ROP) was evaluated. 25 premature neonates (50 eyes) with aggressive posterior ROP were examined and divided into two groups. 13 infants (26 eyes) of the 1st group underwent pattern laser coagulation performed using automanual scanning laser ophthalmologic coagulator.

[Use of perfluorocarbon emulsions for administration of photosensitizing preparations into bone marrow stem cells].

It has been shown that, upon incubation of mouse bone marrow stem cells (BMSC) in vitro with the nanoparticles of perfluorocarbon (PFC) emulsion stabilized by proxanol 268, these nanoparticles penetrate into cells and stay there for a long time (up to 20 days of observation). It has been found that, under in vitro conditions, mouse BMSC loaded with the nanoparticles of both the original emulsion and the emulsion preliminarily incubated with radachlorine do not differ from control stem cells in the rate of division, stretching on a plastic support, and the formation of a monolayer. It has been shown that the exposure to laser radiation of BMSC incubated with the nanoparticles of a PFC emulsion preliminarily incubated with radachlorine under in vitro conditions leads to the death of these cells due to the destruction of the cell membrane.

[Detection of clostridia toxin markers in different types of the course of acute intestinal infections].

The purpose of the investigation was to study the detection rates of markers and the level of C. diffcile A and B toxins and C. perfringens type A enterotoxin in patients with acute intestinal infections (AII).

[Production of recombinant fragment of VacA protein and development of non-invasive method for the diagnosis of Helicobacter pylori infection].

Vacuolizing toxin (VacA) Helicobacter pylori is an important factor of pathogenicity of Helicobacter pylori and a basic marker in the diagnosis of helicobacteriosis and related diseases. A coagulation-based diagnostic test-system was elaborated for the detection of VacA in clinical samples. A fragment of vacA was cloned, for the purpose, in Escherichia coli and expressed in preparative quantities; the coded protein was purified and used in raising the diagnostic serum.

[Production of recombinant fragments of the Clostridium tetani neurotoxin for the development of new immune-prophylaxis preparations against tetanus].

Tetanus belongs to dangerous infection diseases, whose effective prevention can be ensured by vaccines. The acting substance of tetanus vaccines, presently in use, is a partially purified and deprived-of-lethal-action Clostridium tetani neurotoxin. The construction of a subunit preparation on the basis of toxin fragments obtained through gene engineering could be a method aimed at promoting the quality of the used tetanus vaccines.

Construction of a fusion protein carrying antigenic determinants of enteric clostridial toxins.

Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C.

[Gene-engineering approach for the production of A and B toxin fragments for diagnostic and immunotherapeutic use in Clostridium difficile infection].

Clostridium difficle is a causative agent of severe and difficult-to-diagnose human infections. Toxins A and B, which modify the RAS-like proteins of eukaryotic cells, are the major factor in the pathogenicity of the discussed causative agent. These very toxins are considered as the key components of the developed diagnostic and therapeutic-and-preventive preparations.

Purification and characterization of a UDP-glucosyltransferase produced by Legionella pneumophila.

Legionella pneumophila is the agent of Legionnaires' disease. It invades and replicates within eukaryotic cells, including aquatic protozoans, mammalian macrophages, and epithelial cells. The molecular mechanisms of the Legionella interaction with target cells are not fully defined.

[Neurophysiological and psychopharmacological effects of eiconol--a complex of unsaturated fatty acids].

The effect of omega-3 polyunsturated fatty acids on the psychophysiological state of an operator was studied and mechanisms of the drug action were analyzed based on the EEG data. Changes in the psychophysiological and hemodynamic characteristics, EEG, and catecholamine excretion after a 30-day administration of eiconol--a complex of unsaturated fatty acids--in a daily dose of 8 g were studied in a group of 16 middle-aged healthy male operators. It was established that eiconol decreases systolic arterial pressure, reduces adrenalin excretion with urine, imparts the sense of well-being, improves the parameters of activity and the attention stability and concentration, and decreases the level of static tremor.

Actin machinery of phagocytic cells: universal target for bacterial attack.

Uptake of microorganisms by eukaryotic cells depends on proper functioning of the actin machinery. It creates a driving force for the cell membrane deformations necessary for ingestion and killing of microbes by phagocytes. Therefore, specific alterations in the activity of the actin apparatus could be favorable for pathogenic bacteria, representing an efficient mechanism in their virulence.

[Pathogenicity of bacteria and the actin cytoskeleton].

Actin system of eukaryotic cells creates the driving force for alteration of the phagocytic cytoplasmatic membrane shape, which is needed for cell movement in the space and for microorganism capturing. Manipulation by actin cytoskeleton mediated through specialized bacterial products can promote proliferation of bacteria in the host. Published reports indicate that bacterial regulation of the actin system activity can be carried out by two modes: 1) by bacterial interactions with surface receptors regulating the cytoskeleton status and 2) by introduction of bacterial products targeted to the cytoskeleton components into the cells.

[Autoregulation of expression of secreted proteins in Listeria monocytogenes].

The spectrum of proteins secreted by L. monocytogenes greatly depends on the composition of the cultivation medium. The introduction of activated charcoal (AC) into brain heart infusion (BHI) leads to the secretion of a number of additional proteins with mol.

[The use of enterosorbents for preventing and stopping chronic toxic stress in submariners].

The efficiency of prophylactic intestinal sorbents use (algisorb and activated carbon) to prevent chronic toxic stress was investigated in 20 healthy volunteers (atomic submarine personnel) basing on evaluation of central nervous system functional state, hemodynamics, hepatic detoxicative ability and dynamics of trace elements in urine. It was revealed that intestinal sorbents administration in daily dose of 5 g during 20 days promotes gastrointestinal elimination of toxic compounds (cadmium, bismuth, chrome, molybdenum, lithium). It is accompanied with decrease in organism intoxication evidences: reduction in central nervous system excessive excitation, decrease in functional tension of cardiovascular system, recovery of hepatic detoxicative ability.

[Changes in the virulence factor expression level in Listeria monocytogenes under various environmental conditions].

Effects of chelators Chelex-100 and activated charcoal on the production of proteins responsible for virulence of Listeria monocytogenes, facultative intracellular parasite were studied. Bivalent cation chelator Chelex-100 stimulates the production of only thiol-dependent hemolysin listeriolysin O. The presence of activated charcoal, a nonspecific chelator, in culture medium stimulated the expression of listeriolysin O and other main virulence factors by increasing the level of their transcription.

[The therapeutic efficacy and the effect of gidazepam and fenazepam on the psychophysiological status and on the performance of operators with mental disorders at a neurotic level].

The effect of placebo and gidazepam on the state, psychophysiological predictors of the quality of the operator's performance and its results in individuals with neurotic reactions was studied (test dose 20 mg 7-day course, 40 mg daily, 24 individuals), as well as the effect of gidazepam and phenazepam (42 individuals with neurotic and neurosis-like states; gidazepam test doses 20 and 50 mg, 14-day course, 40 and 100 mg daily; phenazepam test doses 0.5 and 1 mg, 14-day course, daily dose 2 mg). The generally accepted methods of clinical and psychophysiological examination were applied.

Software for DNA sequencing by hybridization.

Sequencing by hybridization (SBH) is a promising alternative approach to DNA sequencing and mutation detection. Analysis of the resolving power of SBH involves rather difficult combinatorial and probabilistic problems, and sometimes computer simulation is the only way to estimate the parameters and limitations of SBH experiments. This paper describes a software package, DNA-SPECTRUM, which allows one to analyze the resolving power and parameters of SBH.