Author Correction: Optimization of Photosensitized Tryptophan Oxidation in the Presence of Dimegin-Polyvinylpyrrolidone-Chitosan Systems.

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Optimization of Photosensitized Tryptophan Oxidation in the Presence of Dimegin-Polyvinylpyrrolidone-Chitosan Systems.

By the example of a model process of tryptophan photooxidation in the aqueous medium in the presence of a three-component photosensitizing complex (porphyrin photosensitizer-polyvinylpyrrolidone- chitosan, PPS-PVP-CT) in the temperature range of 20-40 °С, we have demonstrated a possibility of modification of such a process by selecting different molar ratios of the components in the reaction mixture. The actual objective of this selection is the formation of a certain PPS-PVP-CT composition in which PVP macromolecules would coordinate with PPS molecules and at the same time practically block the complex binding of PPS molecules with chitosan macromolecules. Such blocking allows utilization of the bactericidal properties of chitosan to a greater extent, since chitosan is known to depress the PPS photosensitizing activity in PPS-PVP-CT complexes when using those in photodynamic therapy (PDT).

[Influence of dissolved gases on highly diluted aqueous media].

In the experiments on redox potential measurement for a series of identical samples of purified and presettled water it was found that the response to ultraviolet irradiation varies appreciably within a few days after treatment, including stepwise changes. In a few hours after exposure, leading to a higher content of reactive oxygen species as compared with the equilibrium values, long-term changes including variations in redox potential and optical system parameters are recorded in water and diluted aqueous media. We propose a heuristic organization model of the water-gas system with an increased content of reactive oxygen species.

[Effect of acetone and the probe 1-anilino-8-naphthalenesulfonate on water as estimated by fluorescence in the UV region].

For investigation of influence of reactive oxygen species on biological systems the peculiarities of light scattering and fluorescence spectra of aqueous solutions in the presence of photoactive additions of acetone (5 x 10(-4) - 30) and anionic probe ANS (5 microg/l-10 mg/l) have been considered. In water and diluted aqueous acetone and ANS solutions, long-term spontaneous variations in parameters of light scattering and fluorescence spectra have been detected. It has been found that the spectral parameters and the character of ANS influence changed drastically at acetone concentrations of approximately 0.

Isolation and Purification of enzymes from ligninolytic complex of the basidial fungus Trametes pubescens (Schumach.) Pilát and study of their properties.

A method for purification of enzymes from the ligninolityc complex of the basidiomycete Trametes pubescens (Schumach.) Pilat has been elaborated. Two homogeneous isoforms of laccases (laccase 1 and laccase 2) as well as a homogeneous preparation of lignin peroxidase were isolated.

STM study of morphology and electron transport features in cytochrome c and nanocluster molecule monolayers.

The morphology and electron tunneling through single cytochrome c and nanocluster Pt(5)(CO)(7)[P(C(6)H(5))](4) molecules organized as monolayer Langmuir-Blodgett (LB) films on graphite substrate have been studied experimentally using scanning tunneling microscopy (STM) and spectroscopy techniques with sub-nanometer spatial resolution in a double barrier tunnel junction configuration STM tip-monomolecular film-conducting substrate at ambient conditions. STM images of the films revealed globular structures with characteristic diameters (approximately 3.5 nm for the protein molecule and approximately 1.

[Comparative study of the kinetics of the reduction of cytochrome c and the nitroxide radical in reversed aerosol OT micelles in octane].

The comparison of the reduction kinetics of cytochrome c and nitroxide radical by ascorbate in reversed micelles of aerosol OT in octane was studied. The plot of the dependence of the reduction rate constant on the micelle hydratation is bell-shaped in the case of protein but shows the plateau form for the radical. The reaction rates decreases at high micelle concentrations.

[Formation of a complex between carbon monoxide and cytochrome C].

The conditions of structural modifications of horse heart cytochrome c (pH, salt concentration) have been studied. Under these conditions the rate of carboxycytochrome c formation greatly increases in the course of the reduction process as compared to this rate after cytochrome c reduction and relaxation to the equilibrium state. According to these results the reduced intermediate which appears in the course of reduction has a high affinity for the carbon monoxide.