Boosting the toolbox for live imaging of translation.

Live imaging of translation based on tag recognition by a single-chain antibody is a powerful technique to assess translation regulation in living cells. However, this approach is challenging and requires optimization in terms of expression level and detection sensitivity of the system, especially in a multicellular organism. Here, we improved existing fluorescent tools and developed new ones to image and quantify nascent translation in the living embryo and in mammalian cells.

Femtosecond laser writing of robust waveguides in optical fibers with enhanced photosensitivity.

We report the femtosecond laser writing of meter-long optical waveguides inscribed through the coating of specifically designed optical fibers. In order to improve the material photosensitivity and to ensure non-guiding optical fibers for subsequent laser processing of the waveguiding core, a depressed refractive index core design is implemented by co-doping a large portion of the optical fiber with germanium oxide and fluorine. The enhanced photosensitivity provided by further deuterium loading these fibers allows laser-writing of large refractive index contrast waveguides over wide cross sections.

Control of cardiac contractions using Cre-lox and degron strategies in zebrafish.

Cardiac contractions and hemodynamic forces are essential for organ development and homeostasis. Control over cardiac contractions can be achieved pharmacologically or optogenetically. However, these approaches lack specificity or require direct access to the heart.

Ultrafast laser writing of arbitrary long low-loss waveguides in optical fibers.

We propose an innovative femtosecond laser writing approach, based on a reel-to-reel configuration, allowing the fabrication of arbitrary long optical waveguides in coreless optical fibers directly through the coating. We report few meters long waveguides operating in the near-infrared (near-IR) with propagation losses as low as 0.055 ± 0.

Modified astigmatic beam technique for laser writing.

The ultrafast laser writing of optical waveguides and devices is increasingly ubiquitous among the photonics community, mostly for its flexibility and three-dimensional fabrication capability. The well-known astigmatic beam technique is the simplest method to inscribe near-circular cross-section waveguides. In this paper, we report on a significant enhancement to the widely used astigmatic beam technique that makes it more flexible and yields a more circular waveguide cross section.

The control of transcriptional memory by stable mitotic bookmarking.

To maintain cellular identities during development, gene expression profiles must be faithfully propagated through cell generations. The reestablishment of gene expression patterns upon mitotic exit is mediated, in part, by transcription factors (TF) mitotic bookmarking. However, the mechanisms and functions of TF mitotic bookmarking during early embryogenesis remain poorly understood.

Wavefront dislocations reveal the topology of quasi-1D photonic insulators.

Phase singularities appear ubiquitously in wavefields, regardless of the wave equation. Such topological defects can lead to wavefront dislocations, as observed in a humongous number of classical wave experiments. Phase singularities of wave functions are also at the heart of the topological classification of the gapped phases of matter.

100-W-level single-mode ytterbium-free erbium fiber laser.

We report on an ytterbium-free, erbium-doped single-mode all-fiber laser reaching a record output power of 107 W at 1598 nm, with a slope efficiency of 38.6% according to the absorbed pump power at 981 nm. The erbium-doped gain fiber, co-doped with cerium, aluminum, and phosphorus, was fabricated in-house with adjusted doping concentrations to reduce erbium ions clustering, thereby increasing efficiency while keeping the numerical aperture low to ensure a single-mode laser operation.

Direct-laser-written integrated mid-IR directional couplers in a BGG glass.

The development of coherent sources and other optical components for the mid-infrared has been hampered by the lack of sturdy materials that can withstand high power radiation or exposition to harsh environment. BGG glasses are robust materials transmitting over the 2.5-5 μm region.

Cis-regulatory chromatin loops arise before TADs and gene activation, and are independent of cell fate during early Drosophila development.

Acquisition of cell fate is thought to rely on the specific interaction of remote cis-regulatory modules (CRMs), for example, enhancers and target promoters. However, the precise interplay between chromatin structure and gene expression is still unclear, particularly within multicellular developing organisms. In the present study, we employ Hi-M, a single-cell spatial genomics approach, to detect CRM-promoter looping interactions within topologically associating domains (TADs) during early Drosophila development.

Observation of supersymmetric pseudo-Landau levels in strained microwave graphene.

Using an array of coupled microwave resonators arranged in a deformed honeycomb lattice, we experimentally observe the formation of pseudo-Landau levels in the whole crossover from vanishing to large pseudomagnetic field strengths. This result is achieved by utilising an adaptable setup in a geometry that is compatible with the pseudo-Landau levels at all field strengths. The adopted approach enables us to observe the fully formed flat-band pseudo-Landau levels spectrally as sharp peaks in the photonic density of states and image the associated wavefunctions spatially, where we provide clear evidence for a characteristic nodal structure reflecting the previously elusive supersymmetry in the underlying low-energy theory.

Anisotropic nonlinear refractive index measurement of a photorefractive crystal via spatial self-phase modulation.

We show that the refractive index modification photoinduced in a biased nonlinear photorefractive crystal can be accurately measured and controlled by means of a background incoherent illumination and an external electric field. The proposed easy-to-implement method is based on the far-field measurement of the diffraction patterns of a laser beam propagating through a self-defocusing medium undergoing spatial self-phase modulation. For various experimental conditions, both saturation intensity and maximum refractive index modification have been measured.

Broadband integrated beam splitter using spatial adiabatic passage.

Light routing and manipulation are important aspects of integrated optics. They essentially rely on beam splitters which are at the heart of interferometric setups and active routing. The most common implementations of beam splitters suffer either from strong dispersive response (directional couplers) or tight fabrication tolerances (multimode interference couplers).

Remembering the past: Mitotic bookmarking in a developing embryo.

During development, transcriptional properties of progenitor cells are stably propagated across multiple cellular divisions. Yet, at each division, chromatin faces structural constraints imposed by the important nuclear re-organization operating during mitosis. It is now clear that not all transcriptional regulators are ejected during mitosis, but rather that a subset of transcription factors, chromatin regulators and epigenetic histone marks are able to 'bookmark' specific loci, thereby providing a mitotic memory.

Superfluid motion and drag-force cancellation in a fluid of light.

Quantum fluids of light merge many-body physics and nonlinear optics, revealing quantum hydrodynamic features of light when it propagates in nonlinear media. One of the most outstanding evidence of light behaving as an interacting fluid is its ability to carry itself as a superfluid. Here, we report a direct experimental detection of the transition to superfluidity in the flow of a fluid of light past an obstacle in a bulk nonlinear crystal.

ANISEED 2017: extending the integrated ascidian database to the exploration and evolutionary comparison of genome-scale datasets.

ANISEED (www.aniseed.cnrs.

Microwave experiments simulating quantum search and directed transport in artificial graphene.

A series of quantum search algorithms have been proposed recently providing an algebraic speedup compared to classical search algorithms from N to √N, where N is the number of items in the search space. In particular, devising searches on regular lattices has become popular in extending Grover's original algorithm to spatial searching. Working in a tight-binding setup, it could be demonstrated, theoretically, that a search is possible in the physically relevant dimensions 2 and 3 if the lattice spectrum possesses Dirac points.

Selective enhancement of topologically induced interface states in a dielectric resonator chain.

The recent realization of topological phases in insulators and superconductors has advanced the search for robust quantum technologies. The prospect to implement the underlying topological features controllably has given incentive to explore optical platforms for analogous realizations. Here we realize a topologically induced defect state in a chain of dielectric microwave resonators and show that the functionality of the system can be enhanced by supplementing topological protection with non-hermitian symmetries that do not have an electronic counterpart.

Topological transition of Dirac points in a microwave experiment.

By means of a microwave tight-binding analogue experiment of a graphenelike lattice, we observe a topological transition between a phase with a pointlike band gap characteristic of massless Dirac fermions and a gapped phase. By applying a controlled anisotropy on the structure, we investigate the transition directly via density of states measurements. The wave function associated with each eigenvalue is mapped and reveals new states at the Dirac point, localized on the armchair edges.

Faithful communication Hamiltonian in photonic lattices.

Faithful communication is a necessary precondition for large scale all-optical networking and quantum information processing. Related theoretical investigations in different areas of physics have led to various proposals in which finite discrete lattices are used as channels for short-distance communication tasks. Here, in the framework of femtosecond-laser-written waveguide arrays, we present the first experimental realization of such a channel with judiciously engineered couplings.