Elicitation of defense response by transglycosylated chitooligosaccharides in rice seedlings.

Long-chain chitooligosaccharides (COS) with degree of polymerization (DP) more than 4 are known to have potential biological activities. A hyper-transglycosylating mutant of an endo-chitinase from Serratia proteamaculans (SpChiD-Y28A) was used to synthesize COS with DP6 and DP7 using COS DP5 as substrate. Purified COS with DP5-7 were tested to elicit the defense response in rice seedlings.

Pretreatment with KOH and KOH-urea enhanced hydrolysis of α-chitin by an endo-chitinase from Enterobacter cloacae subsp. cloacae.

Chitin is the second most abundant and renewable polysaccharide, next to cellulose. Hydrolysis of abundant and highly crystalline α-chitin, pretreated with KOH and KOH-urea aqueous solutions, by a single modular endo-chitinase from Enterobacter cloacae subsp. cloacae (EcChi1) was investigated.

Applicability of endochitinase of Flavobacterium johnsoniae with transglycosylation activity in generating long-chain chitooligosaccharides.

Chitin and its derivatives are used for a variety of applications. Flavobacterium johnsoniae UW101 is an aerobic Gram-negative bacterium. Genome analysis of F.

Carboxy-terminal glycosyl hydrolase 18 domain of a carbohydrate active protein of Chitinophaga pinensis is a non-processive exochitinase.

The recombinant C-terminal domain of chitinase C of Chitinophaga pinensis (CpChiC-GH18) exhibits the highest activity at pH 6.0 and 35 °C, with a K of 76.13 (mg ml), a k of 10.

Foliar application of brassinosteroids alleviates adverse effects of zinc toxicity in radish (Raphanus sativus L.) plants.

Growth chamber experiments were conducted to investigate the comparative effect of 24-epibrassinolide (EBL) and 28-homobrassinolide (HBL) at 0.5, 1.0, or 2.