Fiber Probe for Simultaneous Mid-Infrared and Fluorescence Spectroscopic Analysis.

A multispectral fiber optic probe has been developed that enables simultaneous analysis of various liquid and solid samples using attenuated total reflection mid-infrared spectroscopy and fluorimetry. The probe design was optimized using ray-tracing simulation of the light propagation. Technical evaluation of the probe has confirmed its output signal quality that was comparable to that of respective probes for single methods.

Synergy Effect of Combined Near and Mid-Infrared Fibre Spectroscopy for Diagnostics of Abdominal Cancer.

Cancers of the abdominal cavity comprise one of the most prevalent forms of cancers, with the highest contribution from colon and rectal cancers (12% of the human population), followed by stomach cancers (4%). Surgery, as the preferred choice of treatment, includes the selection of adequate resection margins to avoid local recurrences due to minimal residual disease. The presence of functionally vital structures can complicate the choice of resection margins.

Evaluation of Discrimination Performance in Case for Multiple Non-Discriminated Samples: Classification of Honeys by Fluorescent Fingerprinting.

In this study we develop a variant of fluorescent sensor array technique based on addition of fluorophores to samples. A correct choice of fluorophores is critical for the successful application of the technique, which calls for the necessity of comparing different discrimination protocols. We used 36 honey samples from different sources to which various fluorophores were added (-(2,2'-bipyridyl) dichlororuthenium(II) (Ru(bpy)), zinc(II) 8-hydroxyquinoline-5-sulfonate (8-Ox-Zn), and thiazole orange in the presence of two types of deoxyribonucleic acid).

Synergy of Fluorescence and Near-Infrared Spectroscopy in Detection of Colorectal Cancer.

Background: Colorectal cancer is one of the most common malignancies worldwide. There is an urgent need for simple and fast methods to improve tumor detection in the diagnostic and intraoperative setting to avoid complications and provide objective information in distinguishing malignant and benign colorectal tissue. Optical spectroscopy methods have recently shown a great potential for this discrimination in different organs.

Synergy Effect of Combining Fluorescence and Mid Infrared Fiber Spectroscopy for Kidney Tumor Diagnostics.

Matching pairs of tumor and non-tumor kidney tissue samples of four patients were investigated ex vivo using a combination of two methods, attenuated total reflection mid infrared spectroscopy and fluorescence spectroscopy, through respectively prepared and adjusted fiber probes. In order to increase the data information content, the measurements on tissue samples in both methods were performed in the same 31 preselected positions. Multivariate data analysis revealed a synergic effect of combining the two methods for the diagnostics of kidney tumor compared to individual techniques.

Development and Testing of an LED-Based Near-Infrared Sensor for Human Kidney Tumor Diagnostics.

Optical spectroscopy is increasingly used for cancer diagnostics. Tumor detection feasibility in human kidney samples using mid- and near-infrared (NIR) spectroscopy, fluorescence spectroscopy, and Raman spectroscopy has been reported (Artyushenko et al., Spectral fiber sensors for cancer diagnostics in vitro.

Reference-free spectroscopic determination of fat and protein in milk in the visible and near infrared region below 1000nm using spatially resolved diffuse reflectance fiber probe.

New technique of diffuse reflectance spectroscopic analysis of milk fat and total protein content in the visible (Vis) and adjacent near infrared (NIR) region (400-995nm) has been developed and tested. Sample analysis was performed through a probe having eight 200-µm fiber channels forming a linear array. One of the end fibers was used for the illumination and other seven - for the spectroscopic detection of diffusely reflected light.

[New species of bacteria in the genus Kurthia--Kurthia sibirica sp. nov].

Six aerobic gram-positive nonspore-forming bacterial strains belonging to the Kurthia genus were isolated from the Magadan (Susuman) mammoth found in the permafrost of the East Siberia. The strains are a phenotypically homogeneous group different from the two known species (K. zopfii and K.

[Morphologic and physiologic-biochemical characteristics of Kurthia zopfii].

Comparative studies were made employing thirteen new isolates of Kurthia zopfii and strain ATCC 10538. It was shown that the cell wall of K. zopfii contained lysine, glycine, alanine, leucine, valine, aspartic and glutamic acids, ribose.

[Formation and structure of oval aggregates of Erwinia herbicola cells].

Formation and structure of oval aggregates were studied by electron and light microscopy of the cells of Erwinia herbicola. Some cells in these cultures were found to synthesize extracellular slime which caused the formation of the aggregates. As has been established by cytochemical techniques, the slime consists mainly of polysaccharides.