[Development of barcode and proteome profiling of glioblastoma].

High grade glioma (glioblastoma) is the most common brain tumor. Its malignancy makes it the fourth biggest cause of cancer death. In our experiments we used several glioblastoma cell lines generated in our laboratory to obtain proteomics information specific for this disease.

[The specific features of the cocirculation of influenza viruses in the 2010-2011 postpandemic period according to the results of activities of the D. I. Ivanovsky Research Institute of Virology, Ministry of Health and Social Development of Russia].

The paper gives the results of monitoring the circulation of influenza viruses in the 2010-2011 season, that covers the second year of circulation of pandemic A(H1N1)v virus strains, and their interaction with seasonal A (H3N2) and B strains. Unlike the previous season, the beginning of an increase in morbidity was recorded in January 2011; its peak in the most of contiguous areas was noted at 5-7 weeks of 2011, with its further decline to threshold levels at week 11 of 2011. Preschool and school children were most involved in the epidemic process.

[Genetic heterogeneity and adaptive strategies of the lady beetle Harmonia axyridis pall. (coleoptera, coccinellidae)].

Analysis of microstatial variability of the phenetic composition of populations of the lady beetle Harmonia axyridis Pall. is performed in Baikal region of the Primorskii Krai. It is revealed that the phenetic composition of the Baikal H.

[Sensitivity of the epidemic and pandemic influenza virus strains to zanamivir (Relenze) in in vitro experiments].

The paper presents the results of the first Russian experience in evaluating the sensitivity of the epidemic and pandemic influenza virus strains, circulating in the period 2009-2010, to the anti-neuraminidase drug zanamivir. A complex of studies, including enzyme immunoassay, fluorometric assay and partial sequence of the neuraminidases (NA1 and NA2) from influenza A virus strain, was applied. The findings Indicate that all the test strains, including those resistant to oseltamivir, were susceptible to zanamivir.

[Molecular genetic studies of the susceptibility of epidemic influenza A(H1N1) virus strains isolated in the 2006-2009 seasons in Russia to oseltamivir (Tamiflu)].

Oseltamivir (Tamiflu) is recommended by WHO experts as a drug to treat and prevent of influenza and to create stocks if its new pandemic variant occurs. The susceptibility of influenza viruses to oseltamivir was studied by polymerase chain reaction-based techniques detecting specific mutations in the neuraminidase gene. The increase in the number of oseltamivir-resistant influenza viruses, isolated from the Russian Federation, with type 1 neuraminidase H274Y mutation from 49% (2007-20008) to 92% (2008-2009) did not depend on the frequency of oseltamivir use.

[Investigation of the interaction of repair DNA polymerase beta and autonomous 3' --> 5'-exonucleases TREX1 and TREX2].

The possibility of interaction of recombinant proteins of human repair DNA polymerase beta with proofreading 3' --> 5'-exonucleases TREX1 and TREX2 was investigated in vitro for the first time. The results of gel filtration analysis show the formation of a complex between 3' --> 5' -exonucleases mTREX1 and hTREX2 and DNA polymerase beta. DNA polymerase activity is shown to increase four-fold in the presence of 3' --> 5'-exonuclease TREX2.

[Nonspecific adaptation reactions and immunological status in patients with type 2 diabetes mellitus].

The clinical laboratory study of 75 patients with type 2 diabetes mellitus (T2D) has shown that most of them have elevated immunoglobulin A and G levels, the diminished activity of neutrophiles, and higher C-reactive protein and 30% of the patients show non-physiological adaptation reactions: reactivation and stress. During these reactions, there are the most pronounced changes in the immunological status and in the level of acute phase protein. The rate of nonphysiological reactions increases, immunity deteriorates, and the activity of an inflammatory process is enhanced with the longer duration of T2D, grades 2 and 3 arterial hypertension, micro- and macroangiopathies, as well as with more evident hyperglycemia and triglyceridemia.

[Spread of new pandemic influenza A(H1N1)v virus in Russia].

The paper presents the results of the investigations of the development of a influenza A(H1N1)v pandemic, conducted by the D. I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences, and collaborating laboratories in the European part of Russia, in the Urals, Siberia, and in the Far East.

[The characteristics of epidemic influenza A and B virus strains circulating in Russia during the 2007-2008 season].

In 2007-2008 in Russia, the epidemic upsurge of influenza morbidity was caused by the active circulation of influenza A(H1N1, A(H3N2), and B viruses. The center for Ecology and Epidemiology of Influenza studied 334 epidemic strains. The results of a comparative study of the svirus specificity of commercial test systems (AmpliSens Influenza virus A/B and AmpliSens Influenza virus A/H5N1) for the polymerase chain reaction diagnosis and virological assays, including virus isolation, revealed their high correlation, which confirms that they may be expensively used to monitor the circulation of influenza viruses in the Russian Federation.

[Monitoring of the sensitivity of epidemic influenza virus strains isolated in Russia to etiotropic chemical agents].

The paper presents the results of studying the spectrum of influenza A and B viruses to rimantadine, arbidol, and oseltamivir and describes the methods used for these purposes for epidemiological surveillance. Different sensitivities to rimantadine were found among influenza A viruses. During the 2007-2008 season, the vast majority of influenza A(H3N2) virus strains were resistant to rimantadine (77%) while all influenza A(H1N1) virus strains preserved their resistance to this drug.

[The 24 May, 2009 isolation of the first A/IIV-Moscow/01/2009 (H1N1)swl strain similar to swine A(H1N1) influenza virus from the first Moscow case detected on May 21, 2009, and its deposit in the state collection of viruses (SCV No. 2452 dated May 24, 2009)].

The paper presents the results of the first isolation of the new influenza virus in Moscow and the Russian Federation, which was similar to the swine A/IIV-Moscow/01/2009(H1N1)swl strain isolated on May 24, 2009 from a Russian arrived in Moscow from the USA on May 19, 2009. The antigenic, biological, and molecular genetic properties of this virus were studied. The virus was isolated on MDCK and chick embryos, the hemagglutination titers being 1:8-1:16 AE; the infectious titers being 6.

[Efficacy of ozeltamivir (Tamiflu) in adult influenza on the epidemic rise of morbidity in Russia in the 2006-2007 season].

The paper gives the results of using ozeltamivir in patients with influenza during the epidemic upsurge of morbidity in Russia in the 2006-2007 season. Comparative analysis of nucteotide sequences of viral strains isolated from the patients taking ozeltamivir revealed no marker mutations determining the resistance to this chemical.

[Complex of repair DNA polymerase beta with autonomous 3'-->5'-exonuclease shows increased accuracy of DNA synthesis].

The complexes of repair DNA polymerase beta with 3'-exonuclease and some other proteins were isolated from the chromatin of hepatocytes of normal rats for the first time. Biopolymers were extracted from the chromatin by the solution of NaCl and Triton X-100. The extract was fractionated by gel-filtration on Sephacryl S-300 columns successively in low and high ionic strength solutions, on hydroxyapatite, and on Sephadex G-100 columns.

[Physical development of children residing in region of goiter endemia].

Data of anthropometrical research organized in the city of Tver and in Tverskaya oblast is presented. Overall, 2949 children and adolescents aged from 7 to 17 years, were examined by endocrinologist on account of diffuse growth of thyroid gland. It is established that most of children (76%) have normal physical development.

[Genotypic characteristics of Mycobacterium tuberculosis strains from the Republic of Tyva].

To determine the genetic heterogenicity of Mycobacterium tuberculosis isolated from patients with tuberculosis from different districts of the Republic of Tyva, 71 M. tuberculosis strains IS6110 according to the polymorphism of restriction fragment lengths were typed; of them 32 strains were spoligotyped. The study could genetically characterize the groups of M.

[Iodocasein in the prophylaxis of iodine deficiency in servicemen].

To evaluate the efficiency of iodcasein in prophylaxis of iodine-deficient states in servicemen the following parameters were studied: iodine sufficiency determined by ioduria median; thyroid volume measured on the base of US data; functional state of hypothalamo-pituitary-thyroid system evaluated by blood level of thyroid hormone and free thyroxin; physical performance assessed by veloergometric test, auto-and-verbal memory and attention change-over. Iodine deficiency was initially detected in 88.9% (main group) and in 94.

[The correcting role of autonomous 3'-->5' exonucleases in mammalian multienzyme DNA polymerase complexes].

A study was made of the correcting role of autonomous 3'-->5' exonucleases (AE) contained in multienzyme DNA polymerase complexes of rat hepatocytes or calf thymocytes. DNA was synthesized on phage psi X174 amber3 or M13mp2 primer-templates, and used to transfect Escherichia coli spheroplasts. Frequencies were estimated for direct and reverse mutations resulting from mistakes made in the course of in vitro DNA synthesis.

[Synthesis of protective antigens during submerged cultivation of Vibrio cholerae].

The effectiveness of dot immunoanalysis for evaluating the dynamics of the synthesis of O-antigen, cholera toxin, neuraminidase, adhesin CFA1 in the process of the reactor cultivation of V. cholerae used for the production of oral chemical cholera vaccine is shown. The established regularities of the synthesis of the protective antigens of V.

[Clinical testing of hygienic criteria for assessing working conditions].

Occupational morbidity in railway transport is discussed. The rates of occupational morbidity are compared with that due to temporary working incapacity. There were no direct correlations between the incidence of major nosological entities of occupational diseases, the magnitude and rate of influence of industrial factors, which provides evidence for their further testing and correction.

[Frequency of chromosome aberrations after irradiation of gametes of cabbage root fly (Delia brassicae Bouche) with various doses of X rays].

Chromosome rearrangements were isolated via crossing of F1 offspring from X-irradiated male and female Delia brassicae with intact insects. An enhanced (more than 30%) rate of late embryonic lethality (LEL) in clutches was the primary criterion of rearrangements. When males alone were irradiated at 2.

[Theory of integrating cellular tension and consequences from it].

Tensegrity model and implications from it have been reviewed. Experimental data confirming the model are shown. While signal vectorization is well confirmed and well depicted by the model, the vectorization of expression implied by the model has not yet been elaborated.

[Improvement in detection, registration and estimation of occupational diseases of the railroad transport].

Misdetection of occupational diseases in railway workers is discussed. The reason is that medical staff has poor knowledge of occupational diseases and patients with their signs and symptoms are not all registered. The paper gives scientific rationale for creating a register of occupational diseases in the system of enterprises subordinate to the Ministry of Railways.