An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design.

Precision DNA/gene replacement is a promising genome-editing tool that is highly desirable for molecular engineering and breeding by design. Although the CRISPR/Cas9 system works well as a tool for gene knockout in plants, gene replacement has rarely been reported. Towards this end, we first designed a combinatory dual-sgRNA/Cas9 vector (construct #1) that successfully deleted miRNA gene regions (MIR169a and MIR827a).

Genome-wide analysis of BURP domain-containing genes in Populus trichocarpa.

BURP domain-containing proteins have a conserved structure and are found extensively in plants. The functions of the proteins in this family are diverse, but remain unknown in Populus trichocarpa. In the present study, a complete genome of P.

Genome-wide analysis of the heat shock transcription factors in Populus trichocarpa and Medicago truncatula.

Research has provided substantial evidences that heat shock proteins (HSPs) play essential roles in extreme physiological conditions. Heat shock transcription factors (HSFs) are important HSPs regulators, but their functions are poorly understood, particularly in Populus and Medicago. In this study, a comprehensive bioinformatics analysis of the HSFs was performed in Populus trichocarpa and Medicago truncatula.