Glyphosate (GLY) and glufosinate (GLUF) are non-selective translocated herbicides that are used in agricultural and non-agricultural land worldwide. The extensive use of GLY and GLUF may lead to their accumulation in soil, which causes soil pollution and affects the soil micro-ecological environment; the accumulated GLY and GLUF also migrate to groundwater via leaching. However, GLY, GLUF, and their metabolites are highly water-soluble and lack chromogenic and fluorescent groups, making them difficult to analyze.
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