Tanshinone I Stimulates Pyroptosis of Cisplatin-Resistant Gastric Cancer Cells by Activating the NF-κB/Caspase-3(8)/GSDME Signaling Pathway.

Cisplatin (DDP) resistance frequently occurs in gastric cancer (GC) therapy. Tanshinone I is a liposoluble phenanthraquinone compound present in the roots of Bunge (Danshen). In this study, we aimed to explore the effects of tanshinone I on modulating DDP resistance of GC cells and .

Quinone Pool, a Key Target of Plant Flavonoids Inhibiting Gram-Positive Bacteria.

Plant flavonoids have attracted increasing attention as new antimicrobial agents or adjuvants. In our previous work, it was confirmed that the cell membrane is the major site of plant flavonoids acting on the Gram-positive bacteria, which likely involves the inhibition of the respiratory chain. Inspired by the similar structural and antioxidant characters of plant flavonoids to hydro-menaquinone (MKH), we deduced that the quinone pool is probably a key target of plant flavonoids inhibiting Gram-positive bacteria.

Stachydrine, a Bioactive Equilibrist for Synephrine, Identified from Four Chinese Herbs.

Four Chinese herbs from the genus, namely Aurantii Fructus Immaturus (), Aurantii Fructus (), Citri Reticulatae Pericarpium Viride () and Citri Reticulatae Pericarpium (), are widely used for treating various cardiovascular and gastrointestinal diseases. Many ingredients have already been identified from these herbs, and their various bioactivities provide some interpretations for the pharmacological functions of these herbs. However, the complex functions of these herbs imply undisclosed cholinergic activity.

Circ_0000190 suppresses gastric cancer progression potentially via inhibiting miR-1252/PAK3 pathway.

Background: Gastric cancer is a serious malignant tumor associated with aberrant circular RNAs (circRNAs) expression. In this study, we aim to investigate the role and the underlying mechanism of circ_0000190, a circRNA in gastric cancer.

Methods: Circ_0000190 expression in vivo was examined in gastric cancer and adjacent normal tissues by RT-PCR.

BIX-01294 enhanced chemotherapy effect in gastric cancer by inducing GSDME-mediated pyroptosis.

Adjuvant chemotherapy in combination with surgery is expected to be a curative strategy for gastric cancer. However, drug resistance remains an obstacle in effective chemotherapy. Therefore, understanding the potential mechanisms of chemotherapy induced gastric cancer cell death is of great importance.

Reactivation of microRNA-506 inhibits gastric carcinoma cell metastasis through ZEB2.

MicroRNAs (miRNAs) are frequently dysregulated in a variety of human cancers, including gastric carcinoma. To improve our understanding of the role of miRNAs in gastric carcinoma and potential identify novel biomarkers or therapeutic agents, we performed microarray analysis to identify differentially expressed miRNAs in gastric carcinoma, compared with paired non-cancerous gastric tissues. We identified significantly differentially expressed miRNAs in gastric carcinoma tissues, including miR-506.

Single Fluorescent Probe Separately and Continuously Visualize HS and HClO in Lysosomes with Different Fluorescence Signals.

A complicated relationship between the active small molecules exists in cells. On the organelle level, active small molecules also play an important role in the maintenance of organelle functions and roles. To investigate the relationship of biomolecules in subcellular, it is necessary and critical to develop molecular tools that can track two kinds of associated biomolecules within organelles with multiple fluorescence signals.

Preparation of a Two-Photon Fluorescent Probe for Imaging HO in Lysosomes in Living Cells and Tissues.

Hydrogen peroxide (HO) plays important roles in many physiological and pathological processes. At the cellular organelle level, the abnormal concentrations of HO in the lysosomes may cause redox imbalance and the loss of the critical functions of the lysosomes. Herein, we describe the preparation of a potent lysosome-targeted two-photon fluorescent probe (Lyso-HP) for the detection of HO in the lysosomes in the living cells.

A targetable fluorescent probe for imaging exogenous and intracellularly formed nitroxyl in mitochondria in living cells.

Nitroxyl plays crucial roles in many biological pathways and can serve as a potent therapeutic agent for the treatment of heart failure. Recent studies suggest that HNO may be produced in mitochondria and the HNO formed might have functional consequences for mitochondrial activity. However, in order to study the function of HNO in mitochondria, a suitable research method is needed.

Single Fluorescent Probe for Dual-Imaging Viscosity and HO in Mitochondria with Different Fluorescence Signals in Living Cells.

Mitochondria, as essential and interesting organelles within the eukaryotic cells, play key roles in a variety of pathologies, and its abnormalities are closely associated with Alzheimer's disease (AD) and other diseases. Studies have shown that the abnormal of viscosity and concentration of hydrogen peroxide in mitochondria were all associated with AD. Accordingly, the detection of viscosity and hydrogen peroxide in mitochondria has attracted great attention.

A lysosome-targeted and ratiometric fluorescent probe for imaging exogenous and endogenous hypochlorous acid in living cells.

Hypochorous acid plays important roles in numerous physiological and pathological processes. At the cell organelle level, an abnormal concentration of hypochorous acid in the lysosomes causes redox imbalance and the loss of function of the lysosomes. Herein, the first small molecule based, lysosomal-targeted ratiometric fluorescent HOCl probe (Lyso-HA) was synthesized through a rational design.

A TICT-based fluorescent probe for rapid and specific detection of hydrogen sulfide and its bio-imaging applications.

By blocking the intramolecular twisted internal charge transfer (TICT) process, we designed and sythesized the first TICT-based fluorescent probe for hydrogen sulfide. The new probe exhibits high selectivity, good membrane-permeability and is suitable for visualization of exogenous and endogenous hydrogen sulfide in living cells.

A fast responsive two-photon fluorescent probe for imaging H₂O₂ in lysosomes with a large turn-on fluorescence signal.

Hydrogen peroxide (H2O2) plays a crucial role in many biological processes in the human body. As our understanding of the complexity of physiological H2O2 in lysosome, investigative tools are required to make sense of this interconnectivity. Toward this goal, we have developed a new example of a fast responsive and lysosome-targeted two-photon H2O2 fluorescent probe (Lyso-HP) with a large turn-on fluorescence signal (80-fold fluorescence enhancement).

A dual-emission fluorescence-enhanced probe for imaging copper(ii) ions in lysosomes.

We have developed the first example of a fluorescence-enhanced and lysosome-targeted Cu probe (Lys-Cu) with unique dual-channel emissions. The newly synthesized fluorescent probe Lys-Cu, which contains two recognition sites with different sensing mechanisms for Cu, displays fluorescence-enhanced dual-channel emissions with fluorescence response to Cu in the lysosome pH environment. Fluorescence imaging shows that Lys-Cu is membrane-permeable and suitable for visualization of Cu in lysosomes of living cells by dual-channel imaging.

Expression of tissue factor pathway inhibitor-2 in gastric stromal tumor and its clinical significance.

The aim of this study was to explore the expression of tissue factor pathway inhibitor-2 (TFPI-2) in gastric stromal tissue and its clinical significance. TFPI-2 expression was detected by immunohistochemical analysis, RT-PCR and western blotting in tumor, peritumoral and gastric normal tissues from 72 patients with gastric stromal tumors. The level of TFPI-2 expression was observed to be significantly higher in gastric normal tissue than in peritumoral tissue, and was significantly higher in peritumoral tissue than in tumor tissue (P<0.

Expression of protease-activated receptor-2 in human gastric stromal tumor and its clinicopathological significance.

Background/aims: To explore the expression of protein-activated receptor-2 (PAR-2) in human gastric stromal tumor and its clinicopathological significance.

Methodology: The expression of PAR-2 was detected with immunohistochemisty, RT-PCR and Western blot in tumor tissue, peritumoral tissue and gastric normal tissue from 72 patients with gastric stromal tumor.

Results: PAR-2 expression was significantly higher in peritumoral tissue (p < 0.