Assessment of Cell Cytotoxicity and Comet Assay on HER2/neu Positive Cell Line Due to In Auger Electrons as DNA-Targeting Radioimmunoconjugate.

Background: Breast cancer Auger electron therapy is a growing field of study in radioimmunotherapy and oncology research. Trastuzumab, a high affinity-binding monoclonal antibody against HER2/neu is which is over-expressed in breast tumors, is used in radiopharmaceutical development.

Objectives: In this work, the lethal effects of In, In-DTPA-trastuzumab and In-trastuzumab coupled-nuclear localizing sequence peptide (In-DTPA-NLS-trastuzumab) on malignant cells were studied in vitro.

The effect of In radionuclide distance and auger electron energy on direct induction of DNA double-strand breaks: a Monte Carlo study using Geant4 toolkit.

Purpose: In this study, the effect of In position and Auger electron energy on direct induction of DSBs was investigated.

Materials And Methods: The Geant4-DNA simulation toolkit was applied using a simple B-DNA form extracted from PDBlib library. First, the simulation was performed for electrons with energies of In and equal emission probabilities to find the most effective electron energies.