Synthesis and characterization of vitamin D-functionalized carbon dots for CRISPR/Cas9 delivery.

To develop a novel nanovector for the delivery of genetic fragments and CRISPR/Cas9 systems in particular. Vitamin D-functionalized carbon dots (D/CDs) fabricated using one-step microwave-aided methods were characterized by different microscopic and spectroscopic techniques. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay and flow cytometry were employed to determine the cell viability and transfection efficiency.