Continuous Glucose Monitoring Underreports Blood Glucose During a Simulated Ultraendurance Run in Eumenorrheic Female Runners.

Purpose: Continuous-glucose-monitoring (CGM) sensors provide near-real-time glucose data and have been introduced commercially as a tool to inform nutrition decisions. The aim of this pilot study was to explore how factors such as the menstrual phase, extended running duration, and carbohydrates affect CGM outcomes among trained eumenorrheic females in an outdoor simulated ultraendurance running event.

Methods: Twelve experienced female ultrarunners (age 39 [6] y) participated in this crossover study.

Comparing Perceived Sleep Quality, Practices, and Behaviors of Male and Female Elite Rugby Union Athletes with the Use of Sleep Questionnaires.

 To evaluate the differences in subjective sleep quality, quantity, and behaviors among male and female elite rugby union athletes through two common sleep questionnaires.  A sample of 38 male and 27 female elite rugby union athletes filled out the Athlete Sleep Behavior Questionnaire (ASBQ) and the Pittsburgh Sleep Quality Index (PSQI). Global scores and individual items for each questionnaire were compared to assess differences between sexes.

Preterm birth and exercise capacity: what do we currently know?

Objectives: The long-term cardiopulmonary outcomes following preterm birth during the surfactant era remain unclear. Respiratory symptoms, particularly exertional symptoms, are common in preterm children. Therefore, cardiopulmonary exercise testing may provide insights into the pathophysiology driving exertional respiratory symptoms in those born preterm.

Reliability of Repeated Nordic Hamstring Strength in Rugby Players Using a Load Cell Device.

Hamstring strain injuries are one of the most common injuries in Rugby Union players, representing up to 15% of all sustained injuries. The Nordic eccentric hamstring test assesses the maximal hamstring eccentric strength and imbalances between limbs. Asymmetries and deficits in hamstring strength between legs are commonly assessed and used as screening methods to prevent injuries which can only be proven effective if hamstring strength measures are reliable over time.

Reliability of Repeated Isometric Neck Strength in Rugby Union Players Using a Load Cell Device.

Concussion is the most common injury in professional Rugby Union (RU) players, with increasing incidence and severity each year. Strengthening the neck is an intervention used to decrease concussion incidence and severity, which can only be proven effective if strength neck measures are reliable. We conducted a repeated-measures reliability study with 23 male RU players.

Hamstrings injury incidence, risk factors, and prevention in Rugby Union players: a systematic review.

Background: Hamstring strain injuries are one of the most common injuries in Rugby Union, representing up to 15% of all injuries sustained. We aimed to systematically review and summarize the scientific literature that addressed hamstring strain injury incidence, risk factors, injury prevention or strengthening strategies, and strength or asymmetry measures in Rugby Union.

Methods: We conducted a systematic search to locate published peer-reviewed articles from PubMed, SPORTDiscus, Web of Science®, and Scopus® e-databases.

Sleep and Performance during a Preseason in Elite Rugby Union Athletes.

Background: Preseason training optimises adaptations in the physical qualities required in rugby union athletes. Sleep can be compromised during periods of intensified training. Therefore, we investigated the relationship between sleep quantity and changes in physical performance over a preseason phase in professional rugby union athletes.

Neck strength in Rugby Union players: a systematic review of the literature.

The incidence and severity of concussion injuries are increasing every year. Scientific evidence indicates that neck strength and girth could play a role in preventing head and neck injuries, or at least mitigating their severity. We aimed to examine the scientific literature addressing neck strength in Rugby Union with a focus on the potential role of neck strength on injury incidence, neck assessment protocols, neck strength measures, and neck strengthening exercises.

Effects of Chronic Cold-Water Immersion in Elite Rugby Players.

Purpose: Although the acute effects of cold-water immersion (CWI) have been widely investigated, research analyzing the effects of CWI over a chronic period in highly trained athletes is scarce. The aim of this study was to investigate the effects of CWI during an intense 3-wk preseason phase in elite rugby athletes.

Methods: A total of 23 elite male rugby union athletes were randomized to either CWI (10 min at 10°C, n = 10) or a passive recovery control (CON, n = 13) during 3 wk of high-volume training.

Mast cells signal their importance in health and disease.

FcεRI is the primary receptor in mast cells that mediates allergic reactions by inducing rapid release of mediators, an adaptive immune response that might have evolved as a host defense against parasites and venoms. Yet it is apparent that mast cells are also activated through non-IgE receptors, the significance of which is just beginning to be understood. This includes the Mas-related G protein-coupled receptor X2, which might contribute to reactions to diverse antimicrobials and polybasic compounds, and the adhesion G protein-coupled receptor E2, variants of which are associated with familial vibratory urticaria and are activated by mechanical vibration.

Bioassay-guided Study of the Antimicrobial and Cytotoxic Properties of the Leaves from Sw.

Background: Sw. () is a plant conventionally used throughout the Caribbean in the treatment of infectious diseases.

Objective: To evaluate, using bioassay-guided fractionation, the cytotoxicity and antimicrobial activity of leaves.

DJ-1 controls bone homeostasis through the regulation of osteoclast differentiation.

Receptor activator of NF-kB ligand (RANKL) generates intracellular reactive oxygen species (ROS), which increase RANKL-mediated signaling in osteoclast (OC) precursor bone marrow macrophages (BMMs). Here we show that a ROS scavenging protein DJ-1 negatively regulates RANKL-driven OC differentiation, also called osteoclastogenesis. DJ-1 ablation in mice leads to a decreased bone volume and an increase in OC numbers.

Interaction of DJ-1 with Lyn is essential for IgE-mediated stimulation of human mast cells.

Background: DJ-1 is a redox-sensitive protein with multiple roles in cell homeostasis, levels of which are altered in patients with mast cell (MC)-related disorders. However, whether DJ-1 can regulate human MC function is unknown.

Objective: We sought to investigate the potential role of DJ-1 in the responses of human MCs to antigen stimulation.

Exon skipping of FcεRIβ eliminates expression of the high-affinity IgE receptor in mast cells with therapeutic potential for allergy.

Allergic diseases are driven by activation of mast cells and release of mediators in response to IgE-directed antigens. However, there are no drugs currently available that can specifically down-regulate mast cell function in vivo when chronically administered. Here, we describe an innovative approach for targeting mast cells in vitro and in vivo using antisense oligonucleotide-mediated exon skipping of the β-subunit of the high-affinity IgE receptor (FcεRIβ) to eliminate surface high-affinity IgE receptor (FcεRI) expression and function, rendering mast cells unresponsive to IgE-mediated activation.

Mesenteric IL-10-producing CD5+ regulatory B cells suppress cow's milk casein-induced allergic responses in mice.

Food allergy is a hypersensitive immune reaction to food proteins. We have previously demonstrated the presence of IL-10-producing CD5(+) B cells and suggested their potential role in regulating cow's milk casein allergy in humans and IgE-mediated anaphylaxis in mice. In this study, we determined whether IL-10-producing CD5(+) regulatory B cells control casein-induced food allergic responses in mice and, if so, the underlying mechanisms.

IL-6 promotes an increase in human mast cell numbers and reactivity through suppression of suppressor of cytokine signaling 3.

Background: IL-6, levels of which are reported to be increased in association with mastocytosis, asthma, and urticaria, is used in conjunction with stem cell factor to generate CD34(+) cell-derived primary human mast cell (HuMC) cultures. Despite these associations, the effects on and mechanisms by which prolonged exposure to IL-6 alters HuMC numbers and function are not well understood.

Objectives: We sought to study the effect of IL-6 on HuMC function, the mechanisms by which IL-6 exerts its effects, and the relationship of these findings to mastocytosis.

Activated mast cells synthesize and release soluble ST2-a decoy receptor for IL-33.

IL-33 released from damaged cells plays a central role in allergic inflammation by acting through its membrane-bound receptor, ST2 receptor (ST2L). IL-33 activity can be neutralized by the soluble spliced variant of ST2 (sST2) that has been associated with allergic inflammation but its source is not well defined. We investigated whether mast cells (MCs) are a significant source of sST2 following activation through FcεRI or ST2.

Interleukin-10-producing CD5+ B cells inhibit mast cells during immunoglobulin E-mediated allergic responses.

Subsets of B cells inhibit various immune responses through their production of the cytokine interleukin-10 (IL-10). We found that IL-10-producing CD5(+) B cells suppressed the immunoglobulin E (IgE)- and antigen-mediated activation of mast cells in vitro as well as allergic responses in mice in an IL-10-dependent manner. Furthermore, the suppressive effect of these B cells on mast cells in vitro and in vivo depended on direct cell-to-cell contact through the costimulatory receptor CD40 on CD5(+) B cells and the CD40 ligand on mast cells.

The CD20 homologue MS4A4 directs trafficking of KIT toward clathrin-independent endocytosis pathways and thus regulates receptor signaling and recycling.

MS4A family members differentially regulate the cell cycle, and aberrant, or loss of, expression of MS4A family proteins has been observed in colon and lung cancer. However, the precise functions of MS4A family proteins and their mechanistic interactions remain unsolved. Here we report that MS4A4 facilitates trafficking of the receptor tyrosine kinase KIT through endocytic recycling rather than degradation pathways by a mechanism that involves recruitment of KIT to caveolin-1-enriched microdomains.

Rictor negatively regulates high-affinity receptors for IgE-induced mast cell degranulation.

Rictor is a regulatory component of the mammalian target of rapamycin (mTOR) complex 2 (mTORC2). We have previously demonstrated that rictor expression is substantially downregulated in terminally differentiated mast cells as compared with their immature or transformed counterparts. However, it is not known whether rictor and mTORC2 regulate mast cell activation.

Comparison of intensities and rest periods for VO2max verification testing procedures.

We sought to determine the incidence of 'true' VO2max confirmation with the verification procedure across different protocols. 12 active participants (men n=6, women n=6) performed in random order 4 different maximal graded exercises tests (GXT) and verification bout protocols on 4 separate days. Conditions for the rest period and verification bout intensity were: A - 105% intensity, 20 min rest; B - 105% intensity, 60 min rest; C - 115% intensity, 20 min rest; D - 115% intensity, 60 min rest.

The influence of the time of day on core temperature and lower body power output in elite rugby union sevens players.

Core temperature typically displays a low circadian in the morning before peaking later in the day, and these changes occur within small physiological ranges. Body temperature plays an important role in physical performance, and some athletes may be required to train and compete in both the morning and evening. However, the influence of the circadian change in body temperature and its influence on physical performance in elite athletes are unclear.

The scaffold protein prohibitin is required for antigen-stimulated signaling in mast cells.

The protein prohibitin (PHB) is implicated in diverse cellular processes, including cell signaling, transcriptional control, and mitochondrial function. We found that PHB was abundant in the intracellular granules of mast cells, which are critical for allergic responses to antigens. Thus, we investigated whether PHB played a role in signaling mediated by the high-affinity receptor for antigen-bound immunoglobulin E (IgE), FcεRI.

Mechanical efficiency of walking with spring-loaded axillary crutches.

The objective of this study was to investigate the effect of using spring-loaded crutches on mechanical efficiency in comparison to the use of standard crutches. Seven healthy male participants were recruited for this experiment. They were instructed to walk around a track on two types of crutches for five minutes under four controlled conditions.