Publications by authors named "Beaudry F"
Optimization of protein identifications through the use of different chromatographic approaches and bioinformatic pipelines.
Rapid Commun Mass Spectrom
January 2025
Rationale: Selection of proteomic workflows for a given project can be a daunting task. This research provides a guide outlining the impact on protein identification of different steps such as chromatographic separation, data acquisition strategies, and bioinformatic pipelines. The data presented here will help experts and nonexpert proteomic users to increase proteome coverage and peptide identification.
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- The Florida Scrub-Jay, a threatened bird species, serves as a significant model for studying evolution and ecology through extensive individual monitoring and genetic analysis.
- Researchers created a detailed linkage map with 4,468 SNPs and a chromosome-level genome assembly for the species, totaling 1.33 Gb in length, which includes complete autosomes and sex chromosomes.
- The high-quality genome assembly, with a BUSCO completeness score of 97.1%, features 17,964 identified protein-coding genes, which equips scientists with valuable resources for exploring the evolutionary dynamics of endangered species.
Objective: Molecular or biomarker testing to guide targeted treatments for colorectal cancer (CRC) has advanced care, specifically by improving treatment specificity. Our objective was to explore patients' experiences and perspectives with biomarker testing in Canada.
Methods: We conducted a mixed-methods study among adults (≥ 18 years) who have been diagnosed with CRC and able to communicate in English.
Article Synopsis
- This study investigated the role of micro-RNAs in understanding pain processing in osteoarthritis by using the MI-RAT model, which simulates arthritis in rats.
- Researchers examined various pain responses, structural damage, and neuropeptide levels in both arthritis-affected and healthy rats over a period of 56 days.
- Results indicated significant cartilage damage and persistent pain hypersensitivity in the arthritis group, along with notable changes in neuropeptide expression and microRNA levels that could inform future pain management strategies.
Article Synopsis
- * Inositol phosphorylceramide (IPC) plays a key role in leishmania species, with IPC synthase (IPCS) being a critical enzyme; clemastine fumarate has emerged as a potential IPCS inhibitor and an anti-leishmanial agent.
- * Research reveals that while clemastine fumarate inhibits IPCS and alters metabolic pathways, its main action may involve different targets, suggesting further investigation is needed for effective treatment strategies.
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- Spinal nociceptive circuits become sensitized in neuropathic pain, largely influenced by gene expression changes through transcriptional and translational controls.
- The study indicates that during chronic neuropathic pain, it's primarily translational regulation that impacts gene expression in the spinal cord.
- Findings show that regulating translation in specific spinal neurons, particularly inhibitory ones, significantly affects pain sensitivity, with increased translation in these neurons linked to heightened hypersensitivity.
Article Synopsis
- The study evaluates the use of fluralaner, an acaricide, to decrease Borrelia burgdorferi reservoirs by targeting infected ticks in key mouse species.
- Fluralaner demonstrated a rapid distribution in mice followed by a slower elimination, achieving a long-lasting effect with doses as low as 10 mg/kg showing protection against tick infestation for an entire active season.
- This research highlights the importance of understanding pharmacology and conducting simulations to enhance the effectiveness of interventions aimed at controlling Lyme disease reservoirs.
Understanding the endocannabinoid system in C. elegans may offer insights into basic biological processes and potential therapeutic targets for managing pain and inflammation in human. It is well established that anandamide modulates pain perception by binding to cannabinoid and vanilloid receptors, regulating neurotransmitter release and neuronal activity.
View Article and Find Full Text PDFSex-biased demography, including sex-biased survival or migration, can alter allele frequency changes across the genome. In particular, we can expect different patterns of genetic variation on autosomes and sex chromosomes due to sex-specific differences in life histories, as well as differences in effective population size, transmission modes, and the strength and mode of selection. Here, we demonstrate the role that sex differences in life history played in shaping short-term evolutionary dynamics across the genome.
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- Visceral leishmaniasis (VL) resolution relies on effective treatments and a functional immune system, but patients on immunosuppressants, like methotrexate (MTX), face greater risks of developing VL and may hesitate to take antileishmanial drugs.* -
- A study investigated the interactions between antimony (Sb) and MTX on different strains of Leishmania infantum in dogs, revealing varied sensitivity and resistance patterns, with some strains displaying cross-resistance.* -
- The findings suggest that the use of immunosuppressants may contribute to the emergence of Sb-MTX resistant parasites, potentially complicating VL treatment in areas where both VL and immunosuppressant use are prevalent.*
Cannabis has gained popularity in recent years as a substitute treatment for pain following the risks of typical treatments uncovered by the opioid crisis. The active ingredients frequently associated with pain-relieving effects are the phytocannabinoids Δ-tetrahydrocannabinol (THC) and cannabidiol (CBD), but their effectiveness and mechanisms of action are still under research. In this study, we used Caenorhabditis elegans, an ideal model organism for the study of nociception that expresses mammal ortholog cannabinoid (NPR-19 and NPR-32) and vanilloid (OSM-9 and OCR-2) receptors.
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- Validating animal pain models is important for improving research and treatment responses, and this study focused on the effects of light exercise combined with pain relief on female rats with induced joint instability.
- Rats were divided into different groups to assess the impact of exercise alone or with the analgesics pregabalin and carprofen over 56 days, showing that exercise increased sensory thresholds and had a synergistic effect with pain relief treatments.
- Histological analysis revealed that while exercise might benefit pain sensitivity, it also led to more significant joint damage compared to sedentary rats, highlighting the complexity of using exercise in pain models for better clinical outcomes.
Article Synopsis
- The study investigates the impact of fluvastatin on the Hippo signalling pathway and its role in breast cancer and canine mammary tumours using a mouse model.
- Four groups of mice were treated with either a vehicle, fluvastatin, doxorubicin, or a combination therapy, and tumor growth and pathology were assessed over 28 days.
- While the combination therapy showed significant differences in tumor volumes, fluvastatin did not affect the Hippo pathway or tumor parameters, and unexpectedly, lung metastases were only observed in the control group, highlighting potential for repurposing statins in veterinary cancer treatment.
Article Synopsis
- Bovine mastitis is a significant infection caused by a pathogen that also affects humans, making antibiotic resistance a critical issue across both species.
- The need for new antibiotics considers the One Health concept, which focuses on the interconnectedness of human, animal, and environmental health.
- PC206, a novel antibiotic with improved stability, has shown effectiveness against the pathogen causing mastitis and is well tolerated in both mice and cows, offering a promising solution for treatment in dairy farms.
Cannflavins isolated from Cannabis sativa impede Caenorhabditis elegans response to noxious heat.
Naunyn Schmiedebergs Arch Pharmacol
January 2024
Cannflavins, flavonoids abundantly present in Cannabis sativa, possess a distinct chemical structure comprising a vanillyl group. Notably, the capsaicin structure also contains a vanillyl group, which is considered essential for interacting with the vanilloid receptor. The vanilloid receptor plays a crucial role in the perception of pain, heat, and inflammation and mediates the analgesic effects of capsaicin.
View Article and Find Full Text PDFThe mGluR orthosteric agonist LY-404,039 reduces dyskinesia, psychosis-like behaviours and parkinsonism in the MPTP-lesioned marmoset.
Naunyn Schmiedebergs Arch Pharmacol
October 2023
Article Synopsis
- LY-404,039 is an agonist for metabotropic glutamate receptors and may also affect dopamine D receptors, making it a candidate for treating conditions like Parkinson’s disease (PD) alongside schizophrenia.
- Prior studies showed that another compound, LY-354,740, improved L-DOPA-induced symptoms in marmosets without activating dopamine receptors, suggesting different therapeutic mechanisms.
- In this study, LY-404,039 significantly reduced dyskinesia and parkinsonism in marmosets already treated with L-DOPA, indicating its potential for repurposing in PD treatment due to its effectiveness.
Article Synopsis
- Janus kinase 3 (JAK3) is part of a protein family important for cell signaling through the JAK/STAT pathway, with its expression differing in bovine granulosa cells, implying it plays a role in their function.
- The research utilized a JAK3 inhibitor (JANEX-1) and FSH treatment to monitor changes in proliferation markers, steroidogenic enzymes, and protein phosphorylation in granulosa cells.
- Results showed that FSH increases JAK3 activity, promoting the expression of certain enzymes and proteins, while JANEX-1 inhibited these actions, indicating JAK3's role in regulating granulosa cell growth and steroid production.
Vanilloids, including capsaicin and eugenol, are ligands of transient receptor potential channel vanilloid subfamily member 1 (TRPV1). Prolonged treatment with vanilloids triggered the desensitization of TRPV1, leading to analgesic or antinociceptive effects. Caenorhabditis elegans (C.
View Article and Find Full Text PDFPharmacokinetic profile of bitopertin, a selective GlyT inhibitor, in the rat.
Naunyn Schmiedebergs Arch Pharmacol
May 2023
Article Synopsis
- Bitopertin, a drug inhibiting glycine transporter 1, has been studied for schizophrenia treatment, especially regarding its safety and tolerability in clinical settings.
- This study aimed to determine the pharmacokinetic (PK) profile of bitopertin in female Sprague-Dawley rats, using various dosing methods and advanced analytic techniques.
- Results showed that bitopertin is slowly absorbed and eliminated, demonstrating a linear relationship between dose and drug exposure, which may help in designing future pre-clinical experiments for subcutaneous administration.
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- * This study investigated how EVs from neutrophils collected during asthma remission and exacerbation impacted ASM proliferation using eight horses with severe asthma, revealing no significant difference based on disease status.
- * Results showed that two proteins (MAST4 and Lrch4) were more abundant in neutrophil EVs during exacerbation, and LPS stimulation enhanced the release of factors that promote ASM proliferation, suggesting that environmental LPS could worsen asthma by activating neutrophils.
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- * Traditional methods of mass spectrometry currently lack the capability to create datasets for future analysis, which limits understanding of unknown compounds present in samples.
- * The article argues for using non-targeted data acquisition methods to build comprehensive digital libraries, enabling long-term data mining and deeper analysis as new questions arise in the field.
Article Synopsis
- Visceral leishmaniasis (VL) is a serious tropical disease with no effective vaccine, relying heavily on chemotherapy, which faces growing challenges due to drug resistance.
- The standard treatment with antimony-based drugs has been in use for over 70 years, leading to significant resistance issues, and similar problems arise with new treatments like miltefosine and amphotericin B.
- Researchers are now using thermal proteome profiling (TPP) to study the effects of these antileishmanial drugs at a molecular level, aiming to identify new drug targets and better understand how resistance develops in the parasites.
Article Synopsis
- The COVID-19 pandemic highlighted the need for efficient analysis methods in public health, leading to the exploration of using LDTD-QqQMS for peptide detection and quantification, particularly focusing on viral proteins.
- Two model peptides and four SARS-CoV-2 specific tryptic peptides were analyzed through LDTD-QqQMS, with quantification achieved by internal calibration under specific experimental conditions.
- The study successfully demonstrated that linear peptides could be qualitatively and quantitatively analyzed using this technique, though the limits of quantification are not yet suitable for clinical use.
Article Synopsis
- Myocardial infarction (MI) is a major risk factor for developing heart failure (HF), and its progression involves interactions between various tissues, including the autonomic nervous system and the medulla oblongata, affecting heart function.
- Denervation of cardiac sympathetic nerves using resiniferatoxin (RTX) shows potential in improving heart function and remodeling after MI, but the molecular processes involved in the MI to HF transition are not fully understood.
- Through proteomic analysis in a mouse model, the study found that metabolic pathways, particularly those related to mitochondria, are significantly affected, suggesting that future experiments should further explore how these metabolic changes can counteract the adverse effects of MI.
A rapid, selective and sensitive method was developed and validated for the determination of LY-404,039 concentration in rat plasma using a butylation derivatization step to improve chromatographic characteristics and enhance signal intensity. The method consisted of a protein precipitation extraction followed by derivatization with butanol/HCl and analysis by high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The separation was achieved using a 100 × 2.
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