Nine high-intensity sweeteners in beverages in a single LC-MS/MS analysis.

With the increasing demand for reduced sugar or sugar-free products by consumers with chronic non-communicable diseases and individuals seeking weight control, foods and beverages with high-intensity sweeteners (HIS) tend to become more common in the market. Therefore, monitoring HIS concentrations added to industrialised products is an important issue. Thus, an analytical method using LC-MS/MS was established and validated in order to allow the simultaneous determination of nine of these compounds in beverages marketed in Brazil.

Exploring pH Dynamics in Amino Acid Solutions Under Low-Temperature Plasma Exposure.

Low-temperature plasma (LTP) offers a promising alternative for cancer therapy, as it targets malignant cells selectively while minimizing damage to healthy tissues. Upon interaction with an aqueous solution, LTP generates reactive oxygen and nitrogen species and thereby influences the solution's pH, which is a crucial factor in cancer proliferation and response to treatment. This study investigated the effects of LTP on the pH of aqueous solutions, with a focus on the effect of LTP parameters such as voltage, frequency, and irradiation time.

Epidemiological landscape of tongue cancer in younger patients in a National Cancer Center in Brazil.

This study investigates the changing epidemiological profile of tongue squamous cell carcinoma (SCC) to young patients, highlighting its rising incidence among non-traditional risk groups. A retrospective descriptive study was conducted, covering data from medical records between 2000 and 2012. Patients were categorised into two age groups (≤ 40 years; 41-50 years).

Hepatotoxic Medicinal Plants in Weight Loss Formulations Sold at Ver-o-Peso Market, Amazon Region, Brazil.

Obesity represents a significant global public health challenge. Various therapeutic strategies for weight reduction are available, including formulations containing medicinal plants, which are favored due to their availability and low cost. The efficacy and safety of these formulations must be evaluated as they can lead to adverse reactions, including severe hepatic injuries.

Maternal Early Overfeeding Negatively Impacts Cardiac Progenitor Cell Differentiation and Cardiomyocyte Maturation in the Neonatal Offspring.

Introduction: Maternal obesity has been positively correlated with an increased cardiometabolic risk in the offspring throughout life, implying intergenerational transmission. However, little is known about the early-life cardiac cell modifications that imply the onset of heart diseases later in life. This study analyzed cardiac progenitor cells and cardiomyocyte differentiation on day of birth in the offspring born to obese dams.

The alteration in myometrial mRNA transcription of the regulatory genes of DNA methylation in mare with endometrosis.

A reduction in myometrial contractile activity can lead to inadequate cleaning of the uterine lumen, resulting in persistent endometritis and potentially endometrosis in mares. Oxytocin (OXT) is a key hormonal regulator of myometrial contraction. While epigenetic regulation of myometrial gene expression has been studied in humans, there is limited information on the expression of DNA methyltransferases (DNMTs) and ten-eleven translocation enzymes (TETs) in the myometrium of mares.

A Case Report on 46,XX Male Difference of Sex Development.

The 46,XX male syndrome is a very rare disorder/difference of sex development (DSD). Characterized by a 46,XX karyotype with a male phenotype and various abnormalities, including virilized external genitalia, small testes, hypergonadotropic hypogonadism, and azoospermia. Primarily described in small population studies and clinical reports, much remains to be understood about the prevalence of clinical manifestations, treatment outcomes, and long-term follow-up in this disorder.

CORE-VTRCC Cruise Report: Geophysical Acquisition and Seafloor Sampling along the Vitória-Trindade Ridge.

This work reports the data obtained by the multidisciplinary cruise CORE-VTRCC surveying on the region around the Vitória-Trindade Ridge (VTR) aboard the vessel NHo Cruzeiro do Sul (October 18 to November 6, 2021). The main objectives of the VTRCC Cruise were (1) to assess the role of the VTR as an oceanographic barrier for bottom currents along the Brazilian margin; and (2) to characterize the morphology of the volcanic seamounts and its relationships with the carbonate sediments that cover it. For that, we performed multibeam bathymetry, magnetometry, high-resolution multichannel seismic survey, subbottom profiling survey, and seafloor sampling along the VTR and along the Columbia Deepwater Channel.

Cadmium Exposure and Noncommunicable Diseases in Environmentally Exposed Brazilian Population: Cross-Sectional Study without Association of Polymorphism.

Cadmium (Cd) is a toxic metal which is harmful to humans and the environment. Cd levels and adverse effects may be associated with genetic polymorphisms in genes involved in its toxicokinetics. This study investigated Cd levels in 198 residents of a condominium in Rio de Janeiro, Brazil, built on industrial steel slag waste and the influence of glutathione S-transferase pi isoform 1 () rs1695 A>G polymorphism.

Preventing Fungal Spoilage from Raw Materials to Final Product: Innovative Preservation Techniques for Fruit Fillings.

Spoilage fungi are a significant cause of financial loss in the food and beverage industry each year. These fungi thrive in challenging environments characterized by low acidity, low water activity and high sugar content, all of which are common in fruit fillings used in pastry products. Fruit fillings are therefore highly susceptible to fungal spoilage.

The loss of riparian vegetation along streams causes morphological divergences in functional traits of semiaquatic insects (Heteropteran: Gerromorpha) in the eastern Amazon.

Understanding the effects of mining activities on Amazonian streams and their impact on aquatic communities is of paramount importance in the current context of resource overexploitation in society. In this study, we assessed the significance of the environment and interspecific interactions on the organization patterns of semiaquatic insect species in a mineral extraction region in the eastern Amazon. We utilized the morpho functional characteristics of 22 species from the suborder Gerromorpha (Heteropteran), considering both the abundance and sexual dimorphism of these species.

Maternal low-dose caffeine intake during the perinatal period promotes short- and long-term sex-dependent hormonal and behavior changes in the offspring.

Aim: Maternal caffeine crosses the placenta and mammary barriers, reaching the baby and, because his/her caffeine metabolism is immature, our hypothesis is that even a low caffeine intake (250 mg/day), lower than the dose limit recommended by the World Health Organization, can promote caffeine overexposure in the offspring, leading to short- and long-term changes.

Main Methods: Pregnant Wistar rats received intragastric caffeine (CAF) (25 mg/Kg/day) or vehicle during the gestation and lactation periods. We evaluated morphometrical, metabolic, hormonal, and behavioral parameters of male and female offspring at different ages.

A great simulator in clinical practice: mononeuritis multiplex in HIV infection.

HIV infection is a chameleon, mimicking several diseases. Herein, we report a previously healthy 39-year-old woman who, over 2 months, developed arthritis, weight loss, and confluent multiple mononeuropathy. Extensive laboratory investigation showed positive serology for HIV, with a CD4 count of 100 cells, and necrotizing vasculitis on a nerve biopsy not associated with CMV co-infection, allowing the diagnosis of polyarteritis nodosa-like vasculitis in an HIV-infected patient.

Determination of high-intensity sweeteners in bakery products marketed in Brazil and dietary exposure assessment.

Bakery products, including biscuits, cakes and breads, generally present a high content of simple sugars of rapid absorption, high fat content and low amount of dietary fiber, which make them highly caloric foods. Although sucrose is a very important ingredient in bakery products for its preservation characteristics and a significant source of energy, there is a growing interest in replacing this sugar with alternative substances, such as high-intensity sweeteners (HIS) that provide sweetness with no or low calories. In Brazil, there is no data on the use of HIS in this class of food.

The fate of Salmonella enterica and Listeria monocytogenes in the pulp of eight native Brazilian and exotic fruits.

Despite the wide variety of native and exotic fruits in Brazil, there is limited understanding of their ability to support pathogens during storage. This study aimed to evaluate the behavior of Salmonella enterica and Listeria monocytogenes inoculated into the pulp of eight fruits native and exotic to Brazil: Jenipapo (Genipa americana L.), Umbu (Spondias tuberosa Arruda), Maná (Solanum sessiliflorum), Cajá-manga (Spondias dulcis), Physalis (Physalis angulata L.

Nonpharmacological Interventions for Treating Breastfeeding Nipple Pain: Systematic Review and Meta-Analysis.

To evaluate the efficacy and safety of nonpharmacological topical interventions for treating breastfeeding nipple pain. Randomized clinical trials (RCTs) assessing lactating women suffering from breastfeeding painful nipples were included. Primary outcomes were pain, healing process, and adverse events.

Chemical profiles and bioactivities of polyphenolic extracts of Lavandula stoechas L., Artemisia dracunculus L. and Ocimum basilicum L.

This study assessed the chemical profiles and bioactivities of the infusions, decoctions and hydroethanolic extracts of tarragon, basil and French lavender. The extracts were chemically characterised (HPLC-DAD-ESI/MS) and their bioactivities were evaluated in vitro. All extracts revealed antimicrobial, antifungal and antioxidant properties.

A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice.

Selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. This study aimed to investigate the and anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyIbis(propane-3-1diyl)] dicarbamate (DD). DD reacted with HOCl ( = 9.

Oestradiol and osteoclast differentiation: Effects on p53 and mitochondrial metabolism.

Background: Oestrogen deficiency increases bone resorption, contributing to osteoporosis development. Yet, the mechanisms mediating the effects of oestrogen on osteoclasts remain unclear. This study aimed to elucidate the early metabolic alteration induced by RANKL, the essential cytokine in osteoclastogenesis and 17-beta-oestradiol (E) on osteoclast progenitor cells, using RAW 264.

Coronary physiology instantaneous wave-free ratio (iFR) derived from x-ray angiography using artificial intelligence deep learning models: a pilot study.

Objectives: Coronary angiography (CAG)-derived physiology methods have been developed in an attempt to simplify and increase the usage of coronary physiology, based mostly on dynamic fluid computational algorithms. We aimed to develop a different approach based on artificial intelligence methods, which has seldom been explored.

Methods: Consecutive patients undergoing invasive instantaneous free-wave ratio (iFR) measurements were included.

Relationship of hemoglobin levels with outcomes in deceased donor kidney transplant: a retrospective cohort study.

Introduction: Anemia is frequent in patients undergoing replacement therapy for kidney failure. Anemia in the pre- and post-transplantation period might be related to kidney transplant outcomes. The current study therefore sought to assess the relationship between anemia, delayed allograft function (DGF), chronic kidney allograft dysfunction (CAD), and death from any cause following kidney transplantation from a deceased donor.

Effect of Hydrothermal Aging on Damping Properties in Sisal Mat-Reinforced Polyester Composites.

Hydrothermal aging is a matter of considerable concern for natural fiber-reinforced polymers; it can alter dimensional stability and induce microcracks and macro strain on the composite structure. This study applied a sorption kinetic model and examined the effects of water on the damping factor of sisal mat-reinforced polyester composites. The experimental data were fitted well using a Boltzmann sigmoid function, suggesting a promising first step toward kinetic water sorption modeling.