Age-dependent expression of Pten and Smad4 genes in the urogenital system of Wistar rats.

Purpose: To analyze Pten and Smad4 gene expression in the urogenital system of Wistar rats in differents ages.

Methods: Pten and Smad4 mRNA expression was assessed in the bladder, ventral prostate, testis, ovaries, and uterus by real-time PCR. Statistical analysis using the ANOVA (p<0.

Glutamine supplementation prevents collagen expression damage in healthy urinary bladder caused by radiotherapy.

Objective: Patients who have had pelvic radiotherapy as part of their cancer therapy may develop subsequent urinary bladder effects such as hyperactive bladder, incontinence, and dysuria. Therefore, the goal of this study was to evaluate whether glutamine supplementation could prevent collagen expression damage in healthy urinary bladder caused by radiotherapy.

Methods: Fifteen adult Wistar rats were separated into a control group that received food and water ad libitum (C group), an irradiated group that received a single pelvic radiation dose of 1164 cGy (I group), and an irradiated group supplemented with l-glutamine every day during the entire experimental period (0.