Paving the way for the use of Statherin-Derived Peptide (StN15) to control caries through acquired pellicle and biofilm microbiome engineering: Proof-of-concept in vitro/in vivo studies.

Objective: This proof-of-concept sequence of in vivo/in vitro studies aimed to unveil the role of acquired enamel pellicle (AEP) engineering with statherin-derived peptide (StN15) on the AEP protein profile, enamel biofilm microbiome in vivo and on enamel demineralization in vitro.

Design: In vivo studies, 10 volunteers, in 2 independent experiments (2 days each), rinsed (10 mL,1 min) with: deionized water (negative control) or 1.88 × 10 M StN15.

Efficacy and safety of TiF varnish in preventing erosive tooth wear in a rat animal model.

An animal model was applied to develop erosive tooth wear (ETW) and to evaluate the efficacy and safety of titanium tetrafluoride (TiF) in preventing ETW. Forty-eight male Wistar rats were divided into three groups (n = 16): TiF (2.45% F), NaF (2.

Influence of irradiated dentin, biofilm and different artificial saliva formulations on root dentin demineralization.

The aim of this study was evaluated the influence of radiation as well as of new formulations of artificial saliva on the development of root dentin lesions. Bovine root samples were divided into: irradiated (70 Gy) dentin or not; the type of biofilm (from irradiated patient-experimental or non-irradiated patient-control) and the type of artificial saliva (for the condition irradiated dentin/biofilm from irradiated patient): Control Artificial Saliva (inorganic); Control Saliva + 1 mg/ml hemoglobin; Control Saliva +0.1 mg/ml cystatin; Control Saliva + hemoglobin + cystatin; Bioextra (positive control) and deionized water (DiW, negative control) (n = 12/group).

Effectiveness of Fluoride Varnishes for White Spot Lesion Prevention and Remineralization during Orthodontic Treatment: A Randomized Controlled Trial.

Introduction: This study investigated the effectiveness of titanium tetrafluoride (TiF4) varnish compared to sodium fluoride (NaF) varnish for preventing and remineralizing white spot lesions (WSLs) in individuals undergoing orthodontic treatment.

Methods: This randomized, placebo-controlled, and double-blinded study was conducted with sixty-five adolescents who were selected based on caries activity and then randomized into three parallel groups: G1 (placebo varnish), G2 (5% NaF varnish), and G3 (4% TiF4 varnish). Volunteers received varnish application weekly for the first 4 weeks, after 6 (T1) and 12 (T2) months.

Acquired pellicle and biofilm engineering with CaneCPI-5: Insights from proteomic and microbiomics analysis.

Objective: In this in vivo proof-of-concept study, acquired pellicle engineering was implemented to promote alterations in the protein composition of the acquired enamel pellicle (AEP) and the bacterial composition of the dental biofilm after treatment with Sugarcane cystatin (CaneCPI-5).

Design: After prophylaxis, 10 volunteers rinsed (10 mL, 1 min) with the following solutions: 1) deionized water (HO- negative control or 2) 0.1 mg/mL CaneCPI-5.

In vitro effect of TiF4/NaF solution on the development of radiation-induced dentin caries.

Objective: To evaluate the protective effect of an experimental solution containing TiF4/NaF on the development of radiation-induced dentin caries lesions.

Methodology: bovine root samples were irradiated (70Gy) and distributed as following (n=12/group): Commercial Saliva (BioXtra), NaF (500 ppm F-), TiF4 (500 ppm F), TiF4/NaF (TiF4: 300 ppm F-, NaF: 190 ppm F-), and Phosphate buffer solution (PBS, negative control). Biofilm was produced using biofilm from irradiated patients and McBain saliva (0.

Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model.

Although the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red photobiomodulation (PBM) as an attractive therapy to downregulate the cytokine storm caused by COVID-19 in a zebrafish model.

The protective effect of the experimental TiF and chitosan toothpaste on erosive tooth wear in vitro.

This study evaluated the protective effect of TiF and chitosan toothpaste on erosive tooth wear (ETW) in vitro. Enamel and dentin samples were randomly assigned to toothpastes (n = 12): (G1) TiF (1400 ppm F), (G2) 0.5% chitosan (75% deacetylation, 500 mPas), (G3) TiF (1400 ppm F) plus 0.

Gdnf Acts as a Germ Cell-Derived Growth Factor and Regulates the Zebrafish Germ Stem Cell Niche in Autocrine- and Paracrine-Dependent Manners.

Glial cell line-derived neurotrophic factor (GDNF) and its receptor (GDNF Family Receptor α1-GFRα1) are well known to mediate spermatogonial stem cell (SSC) proliferation and survival in mammalian testes. In nonmammalian species, Gdnf and Gfrα1 orthologs have been found but their functions remain poorly investigated in the testes. Considering this background, this study aimed to understand the roles of the Gdnf-Gfrα1 signaling pathway in zebrafish testes by combining in vivo, in silico and ex vivo approaches.

Acceptability and effect of TiF4 on dental caries: a randomized controlled clinical trial.

This randomized three-armed controlled clinical trial compared the effect of titanium tetrafluoride (TiF4) and sodium fluoride (NaF) varnishes on caries control in smooth surfaces of permanent dentition and children's acceptability. Sixty children (6-8 y/o) were randomly divided into TiF4 (2.45% F-), NaF (2.

Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation.

Objective: To study the proteomic alterations in the initial AEP after rinsing with CaneCPI-5, StN15 or Hb or their combination.

Materials And Methods: In five crossover phases, after prophylaxis, 10 volunteers in 5 consecutive days, rinsed (10 mL, 1 min) with the following solutions: deionized water (HO- negative control- 1), 0.1 mg/mL CaneCPI-5 (2), 1.

Effect of sweetener containing Stevia on the development of dental caries in enamel and dentin under a microcosm biofilm model.

Objective: This study compared the effect of commercial and pure sweetener containing stevia to that of aspartame, to sucrose and xylitol on the development of dental caries.

Methods: 228 bovine enamel and root dentin were exposed to microcosm biofilm model using human saliva. From the 2 to the 5 day, the samples were exposed daily to McBain saliva supplemented with 0.

Effect of TiF/NaF and chitosan solutions on biofilm formation and prevention of dentin demineralization.

Objective: This study evaluated the effect of experimental solutions containing TiF/NaF and chitosan on bacterial species of microcosm biofilm and on dentin demineralization.

Design: Microcosm biofilm was produced from human saliva mixed with McBain medium (0.2% sucrose) on bovine dentin for 5 days, under 5% CO and 37 °C.

Effect of TiF/NaF and chitosan solutions on the development of enamel caries under a microcosm biofilm model.

Objectives: To evaluate the effect of experimental solutions containing TiF/NaF and chitosan on bacterial species and on enamel caries prevention.

Methods: Microcosm biofilm was produced from human saliva mixed with McBain saliva (0.2% sucrose) on bovine enamel for five days, under 5% CO and 37 °C.

Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro.

This in vitro study evaluated the protective effect of titanium tetrafluoride (TiF) varnish and silver diamine fluoride (SDF) solution on the radiation-induced dentin caries. Bovine root dentin samples were irradiated (70 Gy) and treated as follows: (6 h): 4% TiF varnish; 5.42% NaF varnish; 30% SDF solution; placebo varnish; or untreated (negative control).

A sugarcane cystatin (CaneCPI-5) alters microcosm biofilm formation and reduces dental caries.

The antimicrobial and anticaries effects of CaneCPI-5 were evaluated. Ninety bovine enamel samples were treated for 60 s with either phosphate-buffered-saline (PBS), 0.12% chlorhexidine (CHX), 0.

Effect of TiF varnish after pre-treatment with proanthocyanidin or chlorhexidine on the progression of erosive dentin loss in the presence or absence of the demineralized organic matrix.

This study evaluated the protective effect of TiF varnish, after pre-treatment with proantocyanidin or chlorhexidine, on the progression of erosive dentin loss (EDL), under the presence or absence of the demineralized organic matrix (DOM). Bovine root dentin samples were eroded for 30 min (0.1% citric acid, pH 2.

Effect of chitosan solutions with or without fluoride on the protection against dentin erosion in vitro.

The aim of this study was to assess the protective effect of experimental solutions containing chitosan at different viscosities with or without fluoride (TiF /NaF) on dentin loss in vitro. Bovine dentin samples (n = 15) were prepared and allocated to one of the following treatments: (i) 0.5% chitosan (500 mPas); (ii) 0.

Effect of titanium tetrafluoride/sodium fluoride solutions containing chitosan at different viscosities on the protection of enamel erosion in vitro.

Objective: The aim of this in vitro study was to evaluate the protective effect against enamel erosion of experimental solutions containing TiF/NaF and Chitosan compared to a commercial SnCl/NaF/AmF solution.

Design: Bovine enamel samples were divided (n = 15/group) into: (1) commercial solution SnCl/NaF/AmF (500 ppm F, positive control); (2) NaF/TiF (490 ppm F); (3) similar to 2 plus 0.5 % chitosan (Ch) (500 mPas), (4) similar to 2 plus 0.

Effect of different citrus sweets on the development of enamel erosion in vitro.

The increased consumption of citrus sweets can contribute to the development of erosive tooth wear (ETW). Objective This in vitro study evaluated the erosive potential of citrus sweets on bovine enamel samples regarding the quantification of wear. Methodology Ninety bovine crowns were prepared and samples were randomly distributed into 6 groups (n=15): 0.

Antibiofilm and anti-caries effects of an experimental mouth rinse containing Matricaria chamomilla L. extract under microcosm biofilm on enamel.

Objective: This study evaluated the antibiofilm and anti-caries effects of an experimental mouth rinse containing aqueous extract of Matricaria chamomilla L.

Methods: Microcosm biofilm was produced on bovine enamel, from pooled human saliva mixed with McBain saliva, under 0.2 % sucrose exposure, for 5 days.

The effect of commercial whitening toothpastes on erosive dentin wear in vitro.

Objectives: This study evaluated the erosive tooth wear promoted by commercial whitening toothpastes on eroded dentin in vitro.

Design: Ninety bovine roots were embedded, polished and subjected to the baseline profile analysis. The samples were protected in 2/3 of the dentin surface and were randomly assigned to 6 groups (n = 15/group): Oral-B 3D White; Close-up Diamond Attraction Power White; Sorriso Xtreme White 4D; Colgate Luminous White; Crest and erosion only.

Antimicrobial and Anti-Caries Effect of New Glass Ionomer Cement on Enamel Under Microcosm Biofilm Model.

The occurrence of caries lesions adjacent to restorations is a serious problem in Dentistry. Therefore, new antimicrobial restorative materials could help to prevent recurrent carious lesions. This study evaluated the effect of a new glass ionomer cement (Ion Z) on the viability of a microcosm biofilm and on the development of enamel demineralization.

Effect of an experimental mouth rinse containing NaF and TiF on tooth erosion and abrasion in situ.

Objective: This study compared the effect of an experimental NaF/TiF mouth rinse with a commercial tin/F mouth rinse on the prevention of tooth wear in situ.

Methods: Fifteen subjects took part in this crossover and double-blind study, in which they wore a palatal appliance with 8 bovine teeth samples (4 enamel and 4 root dentine) in each of 3 phases (5 days each). Half of the samples were subjected to erosive challenges, and the other half to erosive plus abrasive challenges.