Publications by authors named "Beata Plitta"

Article Synopsis
  • 5-methylcytosine (m5C) is a key epigenetic marker influencing plant growth, and this study investigates its changes during seed desiccation in Acer platanoides.
  • During the desiccation process from high water content (WC) to lower levels, an initial increase in global DNA methylation was observed, followed by significant demethylation at extremely low WC.
  • The findings suggest that the fluctuations in m5C levels during seed drying may help seeds adapt to desiccation, indicating a potential mechanism for improving seed resilience.
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The effects of storage and deep desiccation on structural changes of DNA in orthodox seeds are poorly characterized. In this study we analyzed the 5-methylcytosine (m(5)C) global content of DNA isolated from seeds of common pear (Pyrus communis L.) that had been subjected to extreme desiccation, and the seedlings derived from these seeds.

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DNA cytosine methylation catalyzed by DNA methyltransferase 1 (DNMT1) is an epigenetic method of gene expression regulation and development. Changes in methylation pattern lead to carcinogenesis. Inhibition of DNMT1 activity could be a good strategy of safe and efficient epigenetic therapy.

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DNA methyltransferase 1 (DNMT1) is one of three enzymes independently coded in mammalian cells. It catalyses postreplicative synthesis of 5-methylcytosine in DNA. The aim of this modification is regulation of gene expression characteristic for the given organism.

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We describe the optimization of a poly(Phe) synthesis system, the conditions of which have been applied for efficient translation of heteropolymeric mRNAs. Here we identify two parameters that are essential to obtain translation at efficiency and accuracy levels equivalent to those in vivo, viz., the fine-tuning of the energy-rich components with an acetyl-phosphate substrate for energy regeneration, as well as the ionic conditions.

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