Publications by authors named "Beata Marianna Kovacs"
Article Synopsis
- Serum amyloid A (SAA) serves as a key acute phase protein and is crucial for diagnosing and monitoring diseases in domestic animals, particularly in cases of secondary amyloidosis in geese.
- A new highly sensitive ELISA method was developed specifically for measuring SAA concentrations in goose serum or plasma, using samples from Landes Grey and Hungarian White geese stimulated by a fowl cholera vaccine.
- The study found that SAA levels peaked at around 1200 microg/ml approximately 24 hours post-inoculation, returning to baseline levels by 72 hours, with less intense responses observed in control groups due to sampling stress.
We defined the nucleotide-sequence of the full-length goose serum amyloid A and compared it to SAA sequences of the duck. The aim of this work was to clone and express recombinant goose SAA and to produce antibody against this protein: Total RNA was isolated from goose liver and used to synthesise first strand cDNA. The coding region of the goose SAA cDNA was amplified by PCR using primers corresponding to the appropriate conservative regions of duck SAA mRNA.
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