Solubility and Thermal Stability of MreB.

The basis of MreB research is the study of the MreB protein from the species, since it was the first one whose crystal structure was described. Since MreB proteins from different bacterial species show different polymerisation properties in terms of nucleotide and salt dependence, we conducted our research in this direction. For this, we performed measurements based on tryptophan emission, which were supplemented with temperature-dependent and chemical denaturation experiments.

Author Correction: Intracellular ion concentrations and cation-dependent remodelling of bacterial MreB assemblies.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Intracellular ion concentrations and cation-dependent remodelling of bacterial MreB assemblies.

Here, we measured the concentrations of several ions in cultivated Gram-negative and Gram-positive bacteria, and analyzed their effects on polymer formation by the actin homologue MreB. We measured potassium, sodium, chloride, calcium and magnesium ion concentrations in Leptospira interrogans, Bacillus subtilis and Escherichia coli. Intracellular ionic strength contributed from these ions varied within the 130-273 mM range.