Publications by authors named "Beata Joanna Lehka"
Background: The yeast Saccharomyces cerevisiae is increasingly used as a cell factory. However, cell factory construction time is a major obstacle towards using yeast for bio-production. Hence, tools to speed up cell factory construction are desirable.
View Article and Find Full Text PDFImproving heterologous production of phenylpropanoids in Saccharomyces cerevisiae by tackling an unwanted side reaction of Tsc13, an endogenous double-bond reductase.
FEMS Yeast Res
January 2017
Article Synopsis
- Scientists are trying to make special chemicals called phenylpropanoids, like flavonoids, using yeast, which is a type of fungus.
- They found that an enzyme called Tsc13, which is important for making fats, is also causing problems by producing an unwanted chemical during this process.
- By making small changes to Tsc13 and using a similar gene from plants, they were able to keep making the good chemicals without creating the unwanted ones.
Dihydrochalcones are plant secondary metabolites comprising molecules of significant commercial interest as antioxidants, antidiabetics, or sweeteners. To date, their heterologous biosynthesis in microorganisms has been achieved only by precursor feeding or as minor by-products in strains engineered for flavonoid production. Here, the native ScTSC13 was overexpressed in Saccharomyces cerevisiae to increase its side activity in reducing p-coumaroyl-CoA to p-dihydrocoumaroyl-CoA.
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