Multiomics approaches disclose very-early molecular and cellular switches during insect-venom allergen-specific immunotherapy: an observational study.

Allergen-specific immunotherapy (AIT) induces immune tolerance, showing the highest success rate (>95%) for insect venom while a much lower chance for pollen allergy. However, the molecular switches leading to successful durable tolerance restoration remain elusive. The primary outcome of this observational study is the comprehensive immunological cellular characterization during the AIT initiation phase, whereas the secondary outcomes are the serological and Th2-cell-type-specific transcriptomic analyses.

Recombinant antigen 5 from Polybia paulista wasp venom (Hymenoptera, Vespidae): Antigen-specific antibody production and functional profile of CD4 T cells in the immune response.

Polybia paulista is a neotropical social wasp related to severe accidents and allergic reactions cases, including anaphylaxis, in southeastern Brazil. Antigen 5 (Poly p 5) is a major allergenic protein from its venom with potential use for component-resolved diagnostic. Therefore, the previous characterization of the immune response profile triggered by Poly p 5 should be evaluated.

Psychosocial adaptation under adverse experiences: A study on the role of empathy with adolescents from vulnerable contexts in Brazil.

Background: Adverse Childhood Experiences (ACEs) are potentially traumatic events that affect children and adolescents, increasing the risk for negative outcomes in several developmental domains. The literature suggests that ACEs affect boys and girls differently, and that protective factors such as social and emotional skills may favor resilience processes and promote Psychosocial Adjustment.

Objective: The present study aimed to verify gender differences in relation to ACEs and Empathy, and to identify whether the effect of ACEs on Psychosocial Adjustment is moderated by Empathy.

Biological and Inflammatory Effects of Antigen 5 from (Hymenoptera, Vespidae) Venom in Mouse Intraperitoneal Macrophages.

The social wasp (Hymenoptera, Vespidae) is highly aggressive, being responsible for many medical occurrences. One of the most allergenic components of this venom is Antigen 5 (Poly p 5). The possible modulation of the in vitro immune response induced by antigen 5 from venom, expressed recombinantly (rPoly p 5), on BALB/c mice peritoneal macrophages, activated or not with LPS, was assessed.

Criminal Behavior and Psychosocial Risk Factors in Brazilian Adolescent Offenders: An Exploratory Latent Class Analysis.

Considering that adolescent offenders are quite a heterogeneous group in terms of behavioral and psychosocial variables, it is considered that a typological approach can assist in the systematization of these differences, aiming at a better understanding of the phenomenon and at clearer guidance in terms of interventions. Therefore, this study aimed to identify the behavioral and psychosocial profiles of male adolescent offenders, based on empirical data collected in a Brazilian sociocultural context. The profiles were made with a sample of 400 adolescent offenders to perform an exploratory latent classes analysis.

Antigen 5 Allergens of Hymenoptera Venoms and Their Role in Diagnosis and Therapy of Venom Allergy.

Purpose Of Review: Stings of Hymenoptera of the superfamily Vespoidea such as yellow jackets, paper wasps or stinging ants are common triggers for severe and even fatal allergic reactions. Antigen 5 allergens are potent allergens in the majority of these venoms with major importance for diagnosis and therapy. Reviewed here are the characteristics of antigen 5 allergens, their role in component-resolved diagnostics as well as current limitations of the available diagnostics for proper therapeutic decisions.

Functional Profile of Antigen Specific CD4 T Cells in the Immune Response to Phospholipase A1 Allergen from Venom.

Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) from wasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen.

Improved production of the recombinant phospholipase A1 from wasp venom expressed in bacterial cells for use in routine diagnostics.

Phospholipase A1 (PLA1) is one of the three major allergens identified in the venom of (Hymenoptera: Vespidae), a clinically relevant wasp from southeastern Brazil. The recombinant form of this allergen (rPoly p 1) could be used for the development of molecular diagnostic of venom allergy. Early attempts to produce rPoly p 1 using BL21 (DE3) cells rendered high yields of the insoluble rPoly p 1 but with low levels of solubilized protein recovery (12%).

Studies on the Relationship between Social Anxiety and Excessive Smartphone Use and on the Effects of Abstinence and Sensation Seeking on Excessive Smartphone Use.

Problematic smartphone use is the excessive use of the smartphone with negative impacts on the quality of life of the user. We investigated the association between social anxiety and excessive smartphone use. The sample consisted of 140 participants, 73 male and 67 female university students with a mean age of 26 years and 4 months (SD = 3.

Current Advances in Immunological Studies on the Vespidae Venom Antigen 5: Therapeutic and Prophylaxis to Hypersensitivity Responses.

Although systemic reactions caused by allergenic proteins present in venoms affect a small part of the world population, Hymenoptera stings are among the main causes of immediate hypersensitivity responses, with risk of anaphylactic shock. In the attempt to obtain therapeutic treatments and prophylaxis to hypersensitivity responses, interest in the molecular characterization of these allergens has grown in the scientific community due to the promising results obtained in immunological and clinical studies. The present review provides an update on the knowledge regarding the immune response and the therapeutic potential of Antigen 5 derived from Hymenoptera venom.

Heterologous Expression, Purification and Immunoreactivity of the Antigen 5 from Polybia paulista Wasp Venom.

(Hymenoptera: Vespidae) is responsible for a high number of sting accidents and anaphylaxis events in Southeast Brazil, Argentina and Paraguay. The specific detection of allergy to the venom of this wasp is often hampered by the lack of recombinant allergens currently available for molecular diagnosis. Antigen 5 (~23 kDa) from venom (Poly p 5) is a highly abundant and glycosylated allergenic protein that could be used for development of component-resolved diagnosis (CRD).

Anti-bullying interventions in schools: a systematic literature review.

This paper presents a systematic literature review addressing rigorously planned and assessed interventions intended to reduce school bullying. The search for papers was performed in four databases (Lilacs, Psycinfo, Scielo and Web of Science) and guided by the question: What are the interventions used to reduce bullying in schools? Only case-control studies specifically focusing on school bullying without a time frame were included. The methodological quality of investigations was assessed using the SIGN checklist.

Molecular cloning, expression and IgE-immunoreactivity of phospholipase A1, a major allergen from Polybia paulista (Hymenoptera: Vespidae) venom.

Polybia paulista (Hymenoptera: Vespidae) is a clinically relevant social wasp that frequently causes stinging accidents in southeast Brazil. To date, diagnosis and specific immunotherapy (SIT) of allergy are based on the use of crude venom extracts. Production of recombinant forms of major allergens from P.

Sexual aggression among Brazilian college students: prevalence of victimization and perpetration in men and women.

Despite the increased attention devoted to sexual aggression among college students in the international research literature, Brazil has no systematic studies on the prevalence of sexual aggression in college populations. The present research measured the prevalence of sexual aggression and victimization since age 14 among 742 first-year college students in Brazil (411 women). A Portuguese version of the Short Form of the Sexual Experiences Survey (Koss et al.

[Violence against children in the city of Ribeirão Preto, São Paulo State, Brazil: child abuse prevalence estimated from school system data].

The official statistics on child abuse fail to reflect reality, since many cases are not reported. The current study aimed to estimate the real prevalence of child abuse in children 0 to 10 years of age enrolled in public and private daycare centers and schools in the city, using information obtained from teachers and staff, based on the premise that they are well-positioned to identify cases, and then to compare this estimated prevalence to the official rate. The instrument used was the Epidemiological Manual, and the random, representative sample consisted of 305 teachers, responsible for 6,907 children.

[Violence against children and adolescents: an analysis of four years of complaints filed at the Child Protection Agency in Ribeirão Preto, São Paulo State, Brazil].

Children and adolescents are exposed to numerous situations of violence. The objective of the current study was to describe such situations qualitatively and quantitatively, based on data from the Child Protection Agency in Ribeirão Preto, São Paulo State, Brazil, for the years 2000 to 2003, aiming to discuss the classification of complaints filed at the agency according to a conceptual framework for violent acts committed against children and adolescents that highlights the structural, criminal, and domestic forms. The study began by tabulating the complaints, organizing them according to the categories used by the agency itself.