Evaluation of immunohistochemical expression of collagen type I in bone defect treated by local application of collagen and beta-tricalcium phosphate in rats.

Objective: Aims: This study aimed to estimate outcome of combined application of collagen/β-tricalcium phosphate (Coll /β- TCP) on bone defect healing by immuno-histochemical localization of collagen type I.

Patients And Methods: Materials and Methods: A total of 20 adult male rats (albino rats) were used in this study. Four intra bony defects were created in both femurs of each rat, three defects were treated with Coll, β- TCP and combination of Coll /β- TCP while forth defect left to heal spontaneously as control.