Publications by authors named "Batya Mannasse-Green"
Telomere Dysfunction in Nonalcoholic Fatty Liver Disease and Cryptogenic Cirrhosis.
Cytogenet Genome Res
February 2017
Nonalcoholic fatty liver disease (NAFLD) and cryptogenic cirrhosis (CC) are considered preneoplastic conditions that might progress to hepatocellular carcinoma. We evaluated parameters of telomere dysfunction in these patient groups to study the correlation between telomere length and the progression of NAFLD. We analyzed peripheral lymphocytes from 22 patients with NAFLD, 20 patients with CC, and 20 healthy, age-matched controls.
View Article and Find Full Text PDFBackground/aims: Non-alcoholic fatty liver disease (NAFLD) and cryptogenic cirrhosis (CC), which is largely a late sequela of NAFLD, are considered pre-neoplastic conditions that might progress to hepatocellular carcinoma. Aneuploidy, telomere aggregates and synchronization of replication were evaluated as markers of genetic instability in these patients.
Methodology: Peripheral blood lymphocytes from 22 patients with NAFLD, 20 patients with CC and 20 age-matched healthy controls were analyzed.
Article Synopsis
- The study introduces a new technique called K-factor that enhances the tracking of fluorescent particles in 3D cellular environments, which aids in understanding cellular processes.
- K-factor reshapes the 3D point spread function (PSF) into a narrower Gaussian profile, increasing localization accuracy by approximately 60% compared to standard Gaussian fitting.
- The effectiveness of the K-factor algorithm was validated through both simulations and experimental data, showing a 50% improvement in resolving overlapping PSFs.