Innate and Adaptive Immunity-Related Markers as Predictors of the Short-Term Progression of Subclinical Atherosclerosis in Middle-Aged Patients.

Assessment of inflammation is a promising approach to monitoring the progression of asymptomatic atherosclerosis. The aim of the present study was to investigate the predictive value of innate and adaptive immunity-related markers, in relation to the short-term progression of subclinical atherosclerosis. The study included 183 patients aged 40-64 years who underwent duplex scanning of the carotid and lower limb arteries at two visits with an interval of 12-24 months between examinations.

Circulating Ageing Neutrophils as a Marker of Asymptomatic Polyvascular Atherosclerosis in Statin-Naïve Patients without Established Cardiovascular Disease.

Background: Current data on the possible involvement of aging neutrophils in atherogenesis are limited. This study aimed to research the diagnostic value of aging neutrophils in their relation to subclinical atherosclerosis in statin-naïve patients without established atherosclerotic cardiovascular diseases (ASCVD).

Methods: The study was carried out on 151 statin-naïve patients aged 40-64 years old without ASCVD.

Associations between Circulating VEGFR2-Neutrophils and Carotid Plaque Burden in Patients Aged 40-64 without Established Atherosclerotic Cardiovascular Disease.

Background: Neutrophils expressing vascular endothelial growth factor receptor (VEGFR) represent a distinct subtype of neutrophils with proangiogenic properties. The purpose of this study was to identify the interrelations between circulating CD16CD11bCD62LCXCR2VEGFR2-neutrophils and indicators of carotid plaque burden in patients without atherosclerotic cardiovascular diseases (ASCVD).

Methods: The study included 145 patients, 51.

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

Background: There is strong evidence to suggest that the negative influence of triglyceride-rich lipoproteins (TRLs) on atherosclerosis development and progression is at least partially mediated by their proinflammatory effects. However, the effect of hypertriglyceridemia (HTG) on the subpopulation composition of circulating neutrophils has not been studied so far. The aim of this study was to examine correlations between the level of triglycerides (TGs) and the subpopulation composition of circulating neutrophils in middle-aged patients with dyslipidemia without established atherosclerotic cardiovascular diseases (ASCVDs).

High Prognostic Value of Mini-Laparoscopy for Advanced Liver Disease-Related Complications in Patients with HCV Infection.

Background/aim: The assessment of advanced chronic liver disease (ACLD) is a prerequisite for therapy and surveillance in patients with chronic hepatitis C infection. Mini-laparoscopy-assisted liver biopsies facilitate both histological and macroscopical evaluation of liver fibrosis. This study is aimed at investigating the prognostic significance of the laparoscopic assessment for the cumulative incidence of ACLD-related events.

[Cytokine status of cervical mucus in women with transitory and persistent course of papillomavirus infection].

Aim: Study the levels of cytokines in cervical mucus of women with transitory and persistent course of papillomavirus infection (PVI).

Materials And Methods: Material from 122 women was studied: 32 patients in control group, 68 patients with transitory course of PVI and 52 women with persistent course of PVI. The presence or absence of high risk HPV DNA was confirmed by real time PCR.

[Prospects of use coryneformic bacteria drug to prevent and treat respiratory infections].

This work shows the Codivac efficiency in its aerosol form to treat children sick of influenza and acute respiratory viral infections. Course of therapy with the Codivac drug leads to improve clinical picture and rapid elimination virus from human organism as compared with children undergone traditional therapy. Recovery is accompanied with improving of indices of cell immunity.

[The humoral immunity against diphtheria].

Results of the conducted study showed that naturally acquired antibacterial and postvaccinal antitoxic antibodies against diphtheria were found in human blood sera. Challenge of ADT-M toxoid to adults resulted in production of antitoxic as well as antibacterial antibodies in high concentrations. In response to challenge of ADT-M toxoid simultaneously with bacterial vaccine against diphtheria Codivac both antibacterial and antitoxic antibodies were synthesized in blood on optimal physiologic levels.

Specificity and efficacy of dendritic cell-based vaccination against tuberculosis with complex mycobacterial antigens in a mouse model.

Dendritic cells (DC) likely play important and unique roles in the generation of protective immunity to mycobacteria. In order to clarify their contributions, bone marrow-derived DC loaded with Mycobacterium tuberculosis sonicate antigens were used to stimulate T cell proliferation both in vitro and in vivo and to vaccinate C57BL/6 mice against subsequent challenge with virulent mycobacteria. Antigen-pulsed DC developed in fetal calf serum (FCS-DC), but not DC developed in normal mouse serum (NMS-DC), stimulated significant proliferation of both naïve and immune T cells in vitro.

Suppression of primary allogenic response by CD8+ memory cells.

Immune response to allogenic tumor cells is associated with the appearance of long-living CD8+ memory cells capable of rapid restimulation and lysis of tumor cells in case of repeated injection of these cells. In order to acquire the effector function, allorestricted memory cells need antigen restimulation for 2 days, which is a specific feature of central memory cell population. These cells can suppress proliferation of naive splenocytes in vitro.

[Immunoloical aspects of the antropogenic response to the antigens of symbiotic non-toxigenic diphtherial corynebacteria].

Corynebacteria, being ancient symbionts of open cavities of human body, carry unique, balanced immunogenic stimuli, formed in the process of evolution, thus maintaining non-specific resistance at a certain level. They favor formation of human microcenotic communities as a normal biological and physiological phenomenon. Codivak, a preparation of natural antigens of a symbiotic strain of non-toxigenic diphtherial corynebacteria, is able to correct not only disturbances of oropharyngeal immunity, but also general cell-mediated and humoral immunity disorders.

[Specific antibodies and their role in the formation of antidiphtheria immunity].

In human sera, studied with the use of the enzyme immunoassay, antidiphtheria postvaccinal antitoxic IgG and naturally acquired antibacterial IgG, IgM and IgA were detected. In the blood of children and adults aged up to 50 years antitoxic IgG were present at normal and high concentrations. In 50% of children antibacterial IgA were absent, while specific antibacterial IgM could be found at high concentrations.

[The determination of lipopolysaccharide admixtures in protein preparations].

A new method for the determination of lipopolysaccharide (LPS) admixtures in protein solutions has been developed. The method includes the periodate oxidation of LPS, biotinylation with biotin hydraside, immobilization on a nitrocellulose membrane and the development of biotinylated LPS in the streptavidin--alkaline phosphatase system. Proteins are previously removed from the solution by treatment with hot phenol.

[Detection of bovine infectious rhinotracheitis virus by hybridization using nonradioactive DNA-probes].

Biotin-labeled DNA probes for infectious bovine rhinotracheitis virus also known as bovine herpesvirus-1 (BHV-1) have been developed. The procedure is based on dot-blot hybridization using biotin-labeled bacteriophage M13 and plasmid probes containing cloned PstI and EcoRI-PstI restriction fragments of viral genome. The probes obtained were used to detect viral nucleic acids in specimens of bovine spermatic fluid or nasal swabs of calves.

[The mutagenic activity of N4-(4-aminobutoxy)-2'-deoxycytidine-5'-triphosphate].

We have shown that deoxycytidine-5'-triphosphate modified by O-(4-aminobutyl)hydroxylamine in the pyrimidine ring, is effectively incorporated into DNA synthesizing in vitro, replacing deoxythymidine-5'-triphosphate or deoxycytidine-5'-triphosphate and inducing A-->G and G-->A transitions, respectively. UV spectroscopy and NMR spectroscopy have shown that the modified cytidine-5'-triphosphate is identical to N4-(4-aminobutoxy)-2'-deoxycytidine-5'-triphosphate. When the modified deoxycytidine-5'-triphosphate was inserted into DNA in vitro by DNA polymerase I of E.

[A method for the primary selection of immunomodulators by the level of 5'-nucleotidase activity in the macrophages of peritoneal exudate].

A method of primary selection of immunomodulators based on measuring of ecto 5'-nucleotidase activity level of macrophages of peritoneal exudate was developed. Immunostimulators were found to decrease the activity level, while immunosuppressors increased the activity. The preparations having no immunomodulatory effect do not change the activity of this enzyme.

[Effect of T-activin on macrophage 5'-nucleotidase activity and blood cortisol level depending on the time of day].

Peculiarities of T-activin influence on macrophage 5-nucleotidase and blood cortisol in dependence of day time have been investigated on CBA and C57/B16 mice. It has been established that T-activin influence on already studied indexes in broad range of doses; from 10 to 1 mkg for 1 mouse.

[Effect of plague microbe fraction 1 protein-polysaccharide complex and pure protein on metabolic parameters of macrophages].

The investigation reveals different influence of the plague microbe's fraction 1 polysaccharide-protein complex and of it's purified protein on the 5'-nucleotidase activity and on the chemiluminescence response of peritoneal macrophages. Both of these metabolic indexes were found to be dependent on the dose of fraction 1 and duration of time till examination.

[Effect of immunomodulators of organometallic nature on the sensitivity to plague toxin].

Influence of immunomodulators with organometallic origin on sensitivity to mice toxin on CBA, C Bl/6 mice and hybrids F1 (CBA C Bl/6) has been investigated. It has been determined that used immunomodulators are the means of urgent change of sensitivity to plague intoxication (5 min after i.v.

[Effect of immobilization stress on macrophage 5'-nucleotidase activity and endogenous cortisol level in mice of the CBA and C57Bl/6 strains].

Complex research of peculiarities of immobilization stress influence on activity of 5'-nucleotidase from macrophages, got from peritoneal exudate and level of endogenous cortisol in blood of CBA and C57Bl/6 mice has been carried out. Genotype peculiarities of macrophage and endocrine index reaction on stress influence have been found.

[Analysis of the immunomodulating action of SK in mice of various strains].

Peculiarities of immunomodulative action of different mice breeds' SK have been determined by changes of activity level of 5'-nucleotidase in macrophages from peritoneal exudate (MPE) and by changes of animals' sensitivity to staphylococcus infection. It has been established that the character of SK influence on activity level of 5'-nucleotidase in MPE and resistance depends on dose, type of administration and the mice breed. Revealed by hypodermic injection, dependence between the activity of 5'-nucleotidase in MPE and sensitivity to staphylococcus infection, whose character was determined by animals' genotype, had straight character in C57Bl/6 mice, and reverse character in CBA mice.