Chitosan/alginate polyelectrolyte complex hydrogels by additive manufacturing for in vitro 3D ovarian cancer modeling.

Polyelectrolyte complexes (PECs) are self-assembled systems formed from oppositely charged polymers, used to create hydrogels for cell culture. This work was aimed at additive manufacturing 3D hydrogels made of a PEC between chitosan (Cs) and alginate, as well as their investigation for in vitro 3D ovarian cancer modeling. PEC hydrogels stability in cell culture medium demonstrated their suitability for long-term cell culture applications.

Functional Rehabilitation in Non-Reconstructed Hemimandibulectomy Patients.

Functional rehabilitation in patients with hemimandibulectomy remains a challenge no matter what method of reconstruction is chosen by physicians. In this paper, we aim to evaluate the feasibility of an acceptable occlusal restoration in patients who have undergone hemimanidublectomy without the reestablishment of mandibular continuity. Data were collected retrospectively on 10 patients with varying degrees of mandible resection.

Evaluation of Protocols for DNA Extraction from Individual to Assess Pyrethroid Resistance Using Genotyping Real-Time Polymerase Chain Reaction.

is a major vector of pathogens, including West Nile and Usutu viruses, that poses a significant public health risk. Monitoring pyrethroid resistance in mosquito populations is essential for effective vector control. This study aims to evaluate four DNA extraction protocols-QIAsymphony, DNAzol Direct reagent, PrepMan Ultra Sample Preparation Reagent (USPR), and Chelex 100-to identify an optimal method to extract DNA from individual , as part of a high-throughput surveillance of pyrethroid resistance using Real-Time Genotyping PCR.

Host plant selection and performance of ambrosia beetles in flood-stressed versus ethanol-injected trees provide implications for management strategies.

Ambrosia beetles (Curculionidae: Scolytinae and Platypodinae) are fungus-farming woodborers that can cause damage to the trees they colonize. Some of these beetles target stressed plants that emit ethanol, and management strategies have proposed using ethanol-injected trees as trap trees to monitor or divert dispersing adult females away from valuable crops. In this study, we used container-grown trees from 8 species to compare the effect of ethanol injection versus flooding on ambrosia beetle host selection and colonization success.

Management of Clinically Negative Neck in Early-Stage (T1-2N0) Oral Squamous-Cell Carcinoma (OSCC): Ten Years of a Single Institution's Experience.

: Oral cavity squamous-cell carcinoma is among the most frequent head and neck neoplasms. Early-stage T1/T2N0 accounts for 40/45% of new diagnoses. Of these, about 30% of cases hide occult metastases in the neck.

A minimally invasive modified revolving-door flap for surgical reconstruction of concha and antihelix defects.

Skin cancers affecting the concha and antihelix are quite common, because of anterior auricular projection from the head and subsequent actinic exposure, leading to the need for effective ear reconstruction post-surgery. Various methods such as skin grafts, free tissue transplantation, and local flaps have been used. This study introduces a refined technique for concha-antihelix defect reconstruction, based on a minimally invasive modification of the revolving-door flap procedure.

GAFchromic EBT film lateral resolution and contrast reproduction in the UV-blue range.

The sensitivity of radiochromic films to UV-blue light is increasingly considered for light dosimetry purposes, owing to their bidimensional detection capabilities and ease of use. While film response to radiation intensity has been widely investigated by commercial scanners, spatial resolution studies remain scarce, especially for small field-of-view applications. These are of growing interest due to the antimicrobial or photo-bio-stimulating effects of UV-blue light sources in in vitro, ex vivo and in vivo models, where precise knowledge of irradiation conditions with adequate spatial resolution is crucial.

Genomics Insights into Causing Infection in a Cat with Pyogranulomatous Dermatitis and Panniculitis.

(homotypic synonym: ) represents an ungrouped thermotolerant rapidly growing mycobacteria (RGM) species occasionally associated with infections and disease in humans. In this report, we describe a case of pyogranulomatous dermatitis and panniculitis due to in an immunocompetent adult cat. To the best of our knowledge, this represents the first report of infection in animals.

Ips typographus vision system: a comprehensive study.

Aggressive bark beetle species such as the Eurasian spruce bark beetle Ips typographus play a fundamental role in forest ecosystems but can also lead to extensive forest mortality and massive economic damage during outbreaks. Currently I. typographus' eyes, visual perception of the world and recognition of specific targets like host plants are understudied topics.

Bone-flap-harvest-related donor site morbidity in reconstructive jaw microsurgery: Retrospective analysis based on 220 patients over a ten-year period.

Microsurgery is the gold standard for hard and soft tissue reconstruction in head and neck neoplasia and malformations. Fibular, iliac crest, and scapular free flaps are the main choices for reconstructive surgery of the jaws. Although widely described in the literature, no statistical comparison analysis of the donor site morbidity of these has been performed to our knowledge.

Time-series sewage metagenomics distinguishes seasonal, human-derived and environmental microbial communities potentially allowing source-attributed surveillance.

Sewage metagenomics has risen to prominence in urban population surveillance of pathogens and antimicrobial resistance (AMR). Unknown species with similarity to known genomes cause database bias in reference-based metagenomics. To improve surveillance, we seek to recover sewage genomes and develop a quantification and correlation workflow for these genomes and AMR over time.

Glycerol-blended chitosan membranes with directional micro-grooves and reduced stiffness improve Schwann cell wound healing.

Regenerative medicine is continuously looking for new natural, biocompatible and possibly biodegradable materials, but also mechanically compliant. Chitosan is emerging as a promising FDA-approved biopolymer for tissue engineering, however, its exploitation in regenerative devices is limited by its brittleness and can be further improved, for example by blending it with other materials or by tuning its superficial microstructure. Here, we developed membranes made of chitosan (Chi) and glycerol, by solvent casting, and micro-patterned them with directional geometries having different levels of axial symmetry.

Commodity risk assessment of maple veneer sheets from Canada.

The European Commission requested the EFSA Panel on Plant Health to deliver a risk assessment on the likelihood of pest freedom from Union quarantine pests and pests subject to measures adopted pursuant to Article 30 of Regulation (EU) No 2016/2031 for the maple veneer sheets manufactured according to the process set out by Canada, with emphasis on the freedom from and (non-EU isolates). The assessment was conducted for veneer sheets of up to 0.7 mm and up to 6 mm thickness, taking into account the different phases in the veneer production in a systems approach.

Compact Spatial Heterodyne Spectrographs for Future Space-Based Observations: Instrument Modeling and Applications.

High-resolution spectroscopy employing spatial heterodyne spectrographs (SHS) holds significant promise for forthcoming space missions, building upon its established track record in science applications. Notably, it offers exceptional performance and cost- effectiveness in the ultraviolet-visual (UV-Vis) region compared to contemporary instruments. SHS instruments provide high-resolution capabilities and substantially larger etendues than similar resolving power instruments.

Joint response from Latin American, European Obstetric Violence Observatories and others organizations all over Europe to the Joint Position Statement on Substandard and Disrespectful Care in Labour - Because Words Matter.

The issue of obstetric violence is internationally acknowledged as a serious violation of human rights. First identified by the Committee of Experts of the Inter-American Belém do Pará Convention in 2012, it is recognized as a form of gender-based violence that infringes upon women's rights during childbirth. Nations such as Argentina, Mexico, Venezuela, and certain regions in Spain have implemented laws against it, highlighting its severity and the need for protective legislation.

Editorial for Special Issue on Biosensors for Biomedical and Environmental Applications.

A sensor is typically defined as a device able to transform a physical quantity of interest into a different kind of signal that can be easily measured and recorded [...

Seasonality of forest insects: why diapause matters.

Insects have major impacts on forest ecosystems, from herbivory and soil-nutrient cycling to killing trees at a large scale. Forest insects from temperate, tropical, and subtropical regions have evolved strategies to respond to seasonality; for example, by entering diapause, to mitigate adversity and to synchronize lifecycles with favorable periods. Here, we show that distinct functional groups of forest insects; that is, canopy dwellers, trunk-associated species, and soil/litter-inhabiting insects, express a variety of diapause strategies, but do not show systematic differences in diapause strategy depending on functional group.

Commodity risk assessment of and plants from the UK.

The European Commission requested the EFSA Panel on Plant Health to prepare and deliver risk assessments for commodities listed in Commission Implementing Regulation (EU) 2018/2019 as 'High risk plants, plant products and other objects'. This Scientific Opinion covers plant health risks posed by plants of and imported from the United Kingdom (UK) as: (a) bundles of budwood/graftwood; (b) 1- to 2-year-old whips, seedlings or transplants; (c) bundles of 1- to 2-year-old cell grown plants; (d) 1- to 7-year-old bare root single plants; and (e) up to 25-year-old single plants in pots, taking into account the available scientific information provided by the UK. A list of pests potentially associated with the commodities was compiled.

Effects of online tDCS and hf-tRNS on reading performance in children and adolescents with developmental dyslexia: a study protocol for a cross sectional, within-subject, randomized, double-blind, and sham-controlled trial.

Background: Developmental Dyslexia (DD) is a brain-based developmental disorder causing severe reading difficulties. The extensive data on the neurobiology of DD have increased interest in brain-directed approaches, such as transcranial direct current stimulation (tDCS), which have been proposed for DD. While positive outcomes have been observed, results remain heterogeneous.

- Italian outbreak management.

, a priority pest for the EU, was first detected in Northern Italy in 2014. Since its discovery, the outbreak extended over an area of more than 16,000 square kilometers in Northern Italy and Southern Switzerland. In this review, we summarize the state-of-the-art of research conducted in Italy on both the spreading capacity and control measures of Chemical, physical, and biological control measures deployed since its detection are presented, by highlighting their strengths and weaknesses.