Effect Of Different Alkaline Treatments of Titanium Surface on Human Osteoblasts Metabolism.

This investigation demonstrates the effect of alkali modification of titanium on the metabolism of human osteoblasts. Polished titanium discs were subjected to alkalinization protocols with NaOH (5M) at 60°C or 120°C. Surface topography and roughness were evaluated using scanning electron microscopy (SEM).

Bifunctional naringenin-laden gelatin methacryloyl scaffolds with osteogenic and anti-inflammatory properties.

Objective: To fabricate and characterize an innovative gelatin methacryloyl/GelMA electrospun scaffold containing the citrus flavonoid naringenin/NA with osteogenic and anti-inflammatory properties.

Methods: GelMA scaffolds (15 % w/v) containing 0/Control, 5, 10, or 20 % of NA w/w were obtained via electrospinning. The chemical composition, fiber morphology/diameter, swelling/degradation profile, and NA release were investigated.

Study of the Failure Mechanism of a High-Density Polyethylene Liner in a Type IV High-Pressure Storage Tank.

The use of Type IV cylinders for gas storage is becoming more widespread in various sectors, especially in transportation, owing to the lightweight nature of this type of cylinder, which is composed of a polymeric liner that exerts a barrier effect and an outer composite material shell that primarily imparts mechanical strength. In this work, the failure analysis of an HDPE liner in a Type IV cylinder for high-pressure storage was carried out. The breakdown occurred during a cyclic pressure test at room temperature and manifested in the hemispherical head area, as cracks perpendicular to the liner pinch-off line.

Effect of moderate hydrostatic pressure on crystallization of palm kernel stearin-sunflower oil model systems.

Lipid crystallization under moderate hydrostatic pressure treatments (200 MPa, 20 °C, 1-24 h) was studied in palm kernel stearin (PS 100%) and its blends with sunflower oil (PS 80, 90 % w/w). Hyperbarically-crystallized samples exhibited significantly higher firmness, elastic modulus and critical stress values as compared to those of the samples crystallized at atmospheric pressure. These data indicate that moderate hydrostatic pressure favored the formation of a higher amount of small palm kernel stearin crystals as compared to those formed at atmospheric pressure.

Influence of titanium and zirconia substrates on the synthesis of inflammatory mediators.

The repair and homeostasis of peri-implant tissues depend on several factors such as the local presence of pathogenic bacteria and their products. Among other events, peri-implant tissue response is also related to the implant material used, which interferes with cells and extracellular matrix interactions, affecting the osseointegration process. In this study, the influence of zirconia (Zr) and titanium (Ti) substrates on the response of preosteoblasts (MC3T3) and murine macrophages (RAW 264.

Crowding on public transport using smart card data during the COVID-19 pandemic: New methodology and case study in Chile.

Most crowding measures in public transportation are usually aggregated at a service level. This type of aggregation does not help to analyze microscopic behavior such as exposure risk to viruses. To bridge such a gap, our paper proposes four novel crowding measures that might be well suited to proxy virus exposure risk at public transport.

EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α.

Objective: This study assessed the metabolism of oral mucosal cells cultured on titanium discs (Ti) coated (or not) with epidermal growth factor (EGF) and exposed to tumor necrosis factor alpha (TNF-α).

Methods: Fibroblasts or keratinocytes were seeded on Ti coated or not with EGF, and then exposed to 100 ng/mL of TNF-α for 24 h. Groups were established: G1: Ti (control); G2: Ti + TNF-α; G3: Ti + EGF; and G4: Ti + EGF + TNF-α.

Naringenin and proanthocyanidins pre-treatment decreases synthesis and activity of gelatinases induced by zoledronic acid in a dental implant surface in vitro model.

Objective: To assess the effects of pre-treatment with proanthocyanidins (PA) flavonoids, from grape seed extract, and synthetic naringenin (NA) on the synthesis of matrix metalloproteinases (MMPs) gelatinases and their tissue inhibitors (TIMPs), as well as the gelatinolytic activity of MMPs by human gingival fibroblasts (HGF) and osteoblasts (Ob) exposed to zoledronic acid (ZA) in a dental implant surface in vitro model.

Design: The highest non-cytotoxic concentrations of NA and PA were determined for HGF (10 μg/mL; defined by previous study) and Ob (0.5 μg/mL; defined by prestoBlue assay).

Estimation of trip purposes in public transport during the COVID-19 pandemic: The case of Santiago, Chile.

The COVID-19 pandemic strongly affected the mobility of people. Several studies have quantified these changes, for example, measuring the effectiveness of quarantine measures and calculating the decrease in the use of public transport. Regarding the latter, however, a low level of understanding persists as to how the pandemic affected the distribution of trip purposes, hindering the design of policies aimed at increasing the demand for public transport in a post-pandemic era.

Voltage mapping of Koch's triangle in atrioventricular nodal reentrant tachycardia ablation : Results from an observational multicenter prospective registry.

Background: The identification of a "low-voltage bridge" to guide ablation of atrioventricular nodal reentry tachycardia (AVNRT) has been described as a safe and effective strategy in children. We investigated the presence of a low-voltage bridge in adult patients undergoing AVNRT ablation, to evaluate its anatomical correspondence with the successful ablation site. We also investigated the possible correlations between Koch's triangle anatomy and patients' biometric characteristics.

Photodynamic therapy for treating infected skin wounds: A systematic review and meta-analysis from randomized clinical trials.

Background: Infected skin wounds represent a public health problem that effects 20 million people worldwide. Photodynamic therapy (PDT) is a treatment option with excellent results against several infections.

Objective: This study aimed to perform a systematic review and meta-analysis on PDT efficacy for treating infected wounds based on randomized clinical trials (RCTs).

Raw milk preservation by hyperbaric storage: Effect on microbial counts, protein structure and technological functionality.

The possibility to apply hyperbaric storage (HS) at room temperature (20 °C) as a sustainable approach for preservation of raw skim milk was studied. Samples were stored at 200 and 150 MPa for up to 6 days. Optimal pressure for milk HS was found to be 150 MPa, since no clotting was detected for up to 6 days.

Titanium alkalinization improves response of osteoblasts to zoledronic acid.

This investigation is aimed to determine the effect of the modification of titanium surface with NaOH on the metabolism of osteoblasts treated with zoledronic acid (ZA). Machined and NaOH-treated titanium disks were used. Surfaces were characterized by scanning electron microscopy, confocal microscopy, and x-ray photoelectron spectroscopy (XPS) analysis.

Regulation of interleukin-6 and matrix metalloproteinases syntheses by bioflavonoids and photobiomodulation in human gingival fibroblasts.

This study aimed to evaluate the separately effects of bioflavonoids proanthocyanidins, from grape seed extract (GSE) and synthetic naringenin (NA), as well as photobiomodulation (PBM) by low-level laser therapy on interleukin (IL)-6 and matrix metalloproteinases (MMPs) syntheses by human gingival fibroblasts (HGF). For this purpose, a connective tissue exposure (ulceration) model of HGF, stimulated with tumor necrosis factor-alpha (TNF-α), was used. Initially, the highest non-cytotoxic and non-genotoxic concentrations of bioflavonoids were determined by cell viability and micronuclei formation assays.

Enterprise application integration task-based execution model experimental dataset.

This data article provides raw log files captured from automated computational experiments with an application integration platform that implements the task-based execution model. The code of a well-known enterprise application integration process was modified to record these data while running the experiments. The dataset described in this article is related to the research article entitled "On the Analysis of Makespan and Performance of the Task-based Execution Model for Enterprise Application Integration Platforms: an Empirical Study" of Frantz et al.

Measuring the transformative utopian impulse for planetary health in the age of the Anthropocene: a multi-study scale development and validation.

Background: Transformative utopian impulse for planetary health is people's propensity to have thoughts and engage in actions of which the purpose is to transform the current society into a better one in the future by addressing existing global issues. We aimed to develop a well-validated scale that can measure the transformative utopian impulse for planetary health and uncover its role in societal transformation.

Methods: We developed a scale to measure the transformative utopian impulse for planetary health across 11 studies with 6248 participants from the USA (from the MTurk database) and the UK (from the Prolifico.

Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique.

Objective: To evaluate the mechanical stability and the proteolytic activity of bonds created by a two-step, etch-and-rinse adhesive applied to cross-linked and air-dried etched dentin.

Methods: Flat dentin surfaces were produced in 64 extracted sound human molars. The dentin was etched with 35% phosphoric acid for 15 seconds, and then the teeth were divided into groups according to the cross-linking solution applied on the etched dentin.

Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells.

Objectives: The present study aimed to assess the oxidative stress and the viability of dental pulp cells stimulated by lipopolysaccharide (LPS) and submitted to photobiomodulation (PBM) with infrared light-emitting diode (LED, 850 nm).

Design: Three healthy primary teeth (n = 3) were collected and seeded in 24-well plates with 10 µg/mL of LPS to induce inflammatory mediator formation. The cells were irradiated (850 nm, 40 mW/cm and 80 mW/cm) at the proposed radiant exposures of 0 (control), 4, 15, and 30 J/cm shortly after LPS supplementation.

A deep learning approach for real-time crash prediction using vehicle-by-vehicle data.

In road safety, real-time crash prediction may play a crucial role in preventing such traffic events. However, much of the research in this line generally uses data aggregated every five or ten minutes. This article proposes a new image-inspired data architecture capable of capturing the microscopic scene of vehicular behavior.

Chemotherapy drugs and inflammatory cytokines enhance matrix metalloproteinases expression by oral mucosa cells.

Objective: Oral mucositis (OM), the most common side effect of cancer therapy, is associated with pro-inflammatory cytokines and matrix metalloproteinases (MMPs) increased expression. Although there are approaches for OM management, none is infallible, thus, elucidation of molecular events related to OM etiopathogenesis may improve current therapeutic strategies. This study assessed the influence of pro-inflammatory cytokines and chemotherapy drugs on MMPs expression and synthesis by oral mucosa cells.

Influence of bisphosphonates on oral implantology: Sodium alendronate and zoledronic acid enhance the synthesis and activity of matrix metalloproteinases by gingival fibroblasts seeded on titanium.

Objective: This study aimed to assess the influence of the bisphosphonates zoledronic acid and sodium alendronate on MMP-2 and MMP-9 synthesis and activity by gingival fibroblasts seeded onto titanium substrate.

Design: Titanium discs were placed in 24-well cell culture plates and gingival fibroblasts were seeded (1 × 10 cells/discs) on them using Dulbecco's Modified Eagle's Medium (DMEM) + 10 % fetal bovine serum (FBS) for 24 h. After this period, a fresh serum-free DMEM containing zoledronic acid or sodium alendronate at 0.

A systematic review and meta-analysis of the effect of photodynamic therapy for the treatment of oral mucositis.

Background: Oral mucositis is a significant reaction to antineoplastic treatment characterized with pain, nutritional compromise, impact on the quality of life, interruption in cancer therapy and risk for infection. There is no effective standard protocol for the treatment of oral mucositis. This study aims to synthesize the scientific evidence available about the effects of photodynamic therapy on treatment of oral mucositis.

Effects of EGF-coated titanium surfaces on adhesion and metabolism of bisphosphonate-treated human keratinocytes and gingival fibroblasts.

Objective: To assess the effects of epidermal growth factor (EGF)-coated titanium (Ti) discs on the adhesion and metabolism of keratinocytes and gingival fibroblasts exposed to nitrogen-containing bisphosphonates.

Materials And Methods: Keratinocytes and fibroblasts were seeded (1 × 10 cells/disc) on Ti discs coated with EGF (100 nM). After 24 h, cells were exposed or not to sodium alendronate (SA) or zoledronic acid (ZA) at different concentrations (0 = control, 0.