Real-time PCR methods for identification and stability monitoring of subsp. UABl-14 during shelf life.

subsp. UABl-14 is an important probiotic strain that was found to support digestive health. Here we present the development and validation of real-time PCR methods for strain-specific identification and enumeration of this important strain.

Real-time polymerase chain reaction methods for strain specific identification and enumeration of strain 8700:2.

Introduction: Reliable and accurate methods for probiotic identification and enumeration, at the strain level plays a major role in confirming product efficacy since probiotic health benefits are strain-specific and dose-dependent. In this study, real-time PCR methods were developed for strain specific identification and enumeration of 8700:2, a probiotic strain that plays a role in fighting the common cold.

Methods: The assay was designed to target a unique region in 8700:2 genome sequence to achieve strain level specificity.