Stem cells are introduced to regenerate some living tissue to restore function and longevity. The study aims to isolate in vitro human periodontal ligament stem cells (hPDLSCs) and investigate their proliferation rate on plasma-treated aligned and random polycaprolactone (PCL) nanofibrous scaffolds made via an electrospinning technique to attempt periodontal-like tissue in dental implants. hPDLSCs were isolated from extracted human premolars and cultured on plasma-treated or untreated PCL-aligned and random scaffolds to enhance adhesion of periodontal ligament (PDL) cells as well as interaction and proliferation.
View Article and Find Full Text PDFThe most common causes of fiber post-failure are inadequate restorations and dislodgement. This study aimed to evaluate fiber post-bond strength to dentin following Er,Cr:YSGG laser irradiation. A total of 65 human mandibular premolars with single roots were included.
View Article and Find Full Text PDFBackground: This study aims to assess the efficacy of Er; Cr: YSGG laser operating under sub-ablative settings for the remineralization of artificially induced white spot lesions on the enamel of primary teeth, both as a standalone treatment and in conjunction with 1.23% acidulated phosphate fluoride (APF) gel.
Material And Methods: Eighty primary posterior teeth were used to induce white spot lesions and were randomly divided into eight groups.
Dental hard and soft tissues have been successfully removed by using the Er,Cr:YSGG laser, but there is a controversy about using lasers over conventional tooth surface preparation for bonding aesthetic restoration. Surface roughness and wettability in response to Er,Cr:YSGG laser irradiation are essential properties for restoration longevity. Fifty-one intact human premolars removed in orthodontic treatment were included in this study and divided into three groups (n=17).
View Article and Find Full Text PDFThe present study was performed to evaluate the influence of a 1064 nm fiber laser on shear bond strength (SBS) at the interface of titanium and resin cement. Forty titanium discs of 6 mm × 3 mm (diameter and thickness respectively) were categorized into four groups (n=10): control group without any surface treatment and three groups treated with a fiber laser with 81 ns pulse duration, 30 kHz frequency, 10000 mm/s scanning speed, 0.05 mm spot size, and different average power values (3, 5 and 7 W) depending on the tested group.
View Article and Find Full Text PDFPurpose: This study investigated periodontal ligament (PDL) restoration in osseointegrated implants using stem cells.
Methods: Commercial pure titanium and zirconium oxide (zirconia) were coated with beta-tricalcium phosphate (β-TCP) using a long-pulse Nd:YAG laser (1,064 nm). Isolated bone marrow mesenchymal cells (BMMSCs) from rabbit tibia and femur, isolated PDL stem cells (PDLSCs) from the lower right incisor, and co-cultured BMMSCs and PDLSCs were tested for periostin markers using an immunofluorescent assay.
Objectives: Dental implant is a revolution in dentistry; some shortages are still a focus of research. This study use long duration of radiofrequency (RF)-magnetron sputtering to coat titanium (Ti) implant with hydroxyapatite (HA) to obtain a uniform, strongly adhered in a few micrometers in thickness.
Materials And Methods: Two types of substrates, discs and root form cylinders were prepared using a grade 1 commercially pure (CP) Ti rod.
Objective: Per-implantitis is one of the implant treatment complications. Dentists have failed to restore damaged periodontium by using conventional therapies. Tissue engineering (stem cells, scaffold and growth factors) aims to reconstruct natural tissues.
View Article and Find Full Text PDFObjectives: This work presents laser coating of grade 1 pure titanium (Ti) dental implant surface with sintered biological apatite beta-tricalcium phosphate (β-TCP), which has a chemical composition close to bone.
Materials And Methods: Pulsed Nd:YAG laser of single pulse capability up to 70 J/10 ms and pulse peak power of 8 kW was used to implement the task. Laser pulse peak power, pulse duration, repetition rate and scanning speed were modulated to achieve the most homogenous, cohesive and highly adherent coat layer.