Evaluation of Random and Aligned Polycaprolactone Nanofibrous Electrospun Scaffold for Human Periodontal Ligament Engineering in Biohybrid Titanium Implants.

Stem cells are introduced to regenerate some living tissue to restore function and longevity. The study aims to isolate in vitro human periodontal ligament stem cells (hPDLSCs) and investigate their proliferation rate on plasma-treated aligned and random polycaprolactone (PCL) nanofibrous scaffolds made via an electrospinning technique to attempt periodontal-like tissue in dental implants. hPDLSCs were isolated from extracted human premolars and cultured on plasma-treated or untreated PCL-aligned and random scaffolds to enhance adhesion of periodontal ligament (PDL) cells as well as interaction and proliferation.

Topographical Analysis of Human Enamel after Phosphoric Acid Etching and Er,Cr:YSGG Laser Irradiation.

Dental hard and soft tissues have been successfully removed by using the Er,Cr:YSGG laser, but there is a controversy about using lasers over conventional tooth surface preparation for bonding aesthetic restoration. Surface roughness and wettability in response to Er,Cr:YSGG laser irradiation are essential properties for restoration longevity. Fifty-one intact human premolars removed in orthodontic treatment were included in this study and divided into three groups (n=17).

Influence of Fiber Laser (1064 nm) on Shear Bond Strength of Titanium Abutment and Resin Cement.

The present study was performed to evaluate the influence of a 1064 nm fiber laser on shear bond strength (SBS) at the interface of titanium and resin cement. Forty titanium discs of 6 mm × 3 mm (diameter and thickness respectively) were categorized into four groups (n=10): control group without any surface treatment and three groups treated with a fiber laser with 81 ns pulse duration, 30 kHz frequency, 10000 mm/s scanning speed, 0.05 mm spot size, and different average power values (3, 5 and 7 W) depending on the tested group.

Bio-hybrid dental implants prepared using stem cells with β-TCP-coated titanium and zirconia.

Purpose: This study investigated periodontal ligament (PDL) restoration in osseointegrated implants using stem cells.

Methods: Commercial pure titanium and zirconium oxide (zirconia) were coated with beta-tricalcium phosphate (β-TCP) using a long-pulse Nd:YAG laser (1,064 nm). Isolated bone marrow mesenchymal cells (BMMSCs) from rabbit tibia and femur, isolated PDL stem cells (PDLSCs) from the lower right incisor, and co-cultured BMMSCs and PDLSCs were tested for periostin markers using an immunofluorescent assay.

Effects of Different Laser Treatments on Some Properties of the Zirconia-Porcelain Interface.

This study was performed to compare the effect of Fractional CO laser or Q switched Nd:YAG laser of surface treatment on the shear bond strength of zirconia-porcelain interface. Fractional CO laser at 30 W, 2 ms, time interval 1 ms, distance between spots 0.3 mm, and number of scans is (4) or Q switched Nd:YAG laser at 30 J/mm and 10 Hz were used to assess the shear bond strength of zirconia to porcelain.

Effects of Long Durations of RF-Magnetron Sputtering Deposition of Hydroxyapatite on Titanium Dental Implants.

Objectives:  Dental implant is a revolution in dentistry; some shortages are still a focus of research. This study use long duration of radiofrequency (RF)-magnetron sputtering to coat titanium (Ti) implant with hydroxyapatite (HA) to obtain a uniform, strongly adhered in a few micrometers in thickness.

Materials And Methods:  Two types of substrates, discs and root form cylinders were prepared using a grade 1 commercially pure (CP) Ti rod.

Preparing polycaprolactone scaffolds using electrospinning technique for construction of artificial periodontal ligament tissue.

Objectives: The strategies of tissue-engineering led to the development of living cell-based therapies to repair lost or damaged tissues, including periodontal ligament and to construct biohybrid implant. This work aimed to isolate human periodontal ligament stem cells (hPDLSCs) and implant them on fabricated polycaprolactone (PCL) for the regeneration of natural periodontal ligament (PDL) tissues.

Methods: hPDLSCs were harvested from extracted human premolars, cultured, and expanded to obtain PDL cells.

Implementation and characterization of coating pure titanium dental implant with sintered β-TCP by using Nd:YAG laser.

Objectives: This work presents laser coating of grade 1 pure titanium (Ti) dental implant surface with sintered biological apatite beta-tricalcium phosphate (β-TCP), which has a chemical composition close to bone.

Materials And Methods: Pulsed Nd:YAG laser of single pulse capability up to 70 J/10 ms and pulse peak power of 8 kW was used to implement the task. Laser pulse peak power, pulse duration, repetition rate and scanning speed were modulated to achieve the most homogenous, cohesive and highly adherent coat layer.