Publications by authors named "Basil Baby Mattamana"
Article Synopsis
- Organ transplant recipients on immunosuppressants have a weakened ability to respond to infections, including SARS-CoV-2, but their antibody responses may still be effective.
- A new mass spectrometry technique called Ig-MS was used to compare immune responses to COVID-19 between transplant recipients and immunocompetent controls at a single point and over a month after diagnosis.
- The study found no significant differences in antibody characteristics like titer, clonality, or glycan composition between the two groups, suggesting that the immune response evolution in transplant recipients resembles that of immunocompetent individuals.
Although vaccination efforts have expanded, there are still gaps in our understanding surrounding the immune response to SARS-CoV-2. Measuring IgG Fc glycosylation provides insight into an infected individual's inflammatory state, among other functions. We set out to interrogate bulk IgG glycosylation changes from SARS-CoV-2 infection and vaccination, using plasma from mild or hospitalized COVID-19 patients, and from vaccinated individuals.
View Article and Find Full Text PDFMethods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses recent advances in protein mass spectrometry (MS) for multiparametric readout of antibodies, with new metrics like Ion Titer (IT) and Degree of Clonality (DoC) capturing the heterogeneity and relative abundance of individual clones without sequencing of B cells.
View Article and Find Full Text PDFMethods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS , a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses recent advances in protein mass spectrometry (MS) for multi-parametric readout of antibodies, with new metrics like Ion Titer (IT) and Degree of Clonality (DoC) capturing the heterogeneity and relative abundance of individual clones without sequencing of B cells.
View Article and Find Full Text PDF