Structural and Functional Analysis of the Spermatogenic Epithelium in Rats Exposed to Titanium Dioxide Nanoparticles.

We studied immunohistochemical and morphometric characteristics of the spermatogenic epithelium in rats against the background of peroral administration of nanoparticles containing titanium dioxide. Substantial degenerative changes in the spermatogenic epithelium were revealed: thinning, disorganization of layers, and detachment of sperm cells from the basement membrane. Immunohistochemical analysis revealed reduced proliferative activity and differentiation potential of epithelial cells, which was confirmed by changes in the expression of Ki-67 and c-kit markers.

[Glycosaminoglycans dysbolism in pathogenesis of idiopathic varicocele].

Ultrasound dopplerography (UD) was used to examine 50 patients with idiopathic varicocele (IV) and 20 healthy subjects. Such parameters were assessed as age, severity of IV, its duration, scrotal vessels UD data, vein diameter of the pampiniform plexus on the left, reflux at the height of the Valsalva test, uronic acids (UA), glycosaminoglycanes (GAG) and their fractions (hialuronic acid--HA, chondroitin sulphates--CS, heparansulphates--HS). UA and GAG fraction composition was studied with ion exchange chromatography.

[Interaction of nucleic acids and glycans].

Spectrophotometric analysis and dot-hybridization have shown that amylose forms complexes with polypyrimidines (poly dC), while polyuronides form complexes with polypurines (poly dA). In addition, the formation of complexes genomic thymus DNA-hyaluronic acid has been observed. A certain role in the mechanism of NA-polysaccharide interactions can be played by the links between purines and the carboxylic group of hexuronic acid residue, as well as between pyrimidines and the hydroxymethyl group of hexose residue.

[Glycosaminoglycanes biosynthesis connection with nuclear and microsomal cell apparatus].

The polysaccharides fractional composition was characterized in rat liver. Glycosaminoglycanes (GAG) anabolism was studied with the help of 14C1-glucose used as the synthesis precursor. It was demonstrated that GAG polysaccharide fragments synthesis began in the cell nucleus associated structures and finished in the microsomal cell fraction.

[Biopolymers in urine of patients with syringomyelia].

Urine of patients with syringomyelia was analysed by gel filtration, ion-exchange chromatography and analytical chemistry methods. Increased level of acidic glycoproteins and decreased level glycosaminoglycans levels were found. Patients with syringomyelia had smaller glycosaminoglycans components of proteoglycans than normal subjects.

[Content and metabolism of glycosaminoglycanes in organs and tissues of different aged white rats].

Glycosaminoglycanes content was decreased in liver, brain and skin of white rats during ageing due to the decrease of non-sulfated fraction. Sulfated glycosaminoglycanes synthesis intensity was decreased too. These pathochemical processes were more expressed in skin and liver than in brain.

[Are-related changes in content of glycosaminoglycans in female skin].

Postmortem skin samples of 50 women aged from 15 to 90 years were analysed. Protein and DNA levels were stable and rather age-independent. Glycosaminoglycane (GAG) level decreased during ageing according to linear regression equation: Age = 100.

[Age changes in glycosaminoglycan level in the woman skin].

Postmortem skin samples of 50 women aged from 15 to 90 years were analysed. Protein and DNA levels were stable rather age-independent. Glycosaminoglycane (GAG) level decreased during ageing accordingly linear regression equation: Age = 100.

[Anti-inflammatory activity of hyaluronic acid].

The results of in vivo experiments showed that hyaluronic acid possesses antiexudative activity comparable with that of nonsteroidal anti-inflammatory drugs. Hyaluronic acid exhibits antagonism with respect to histamine-induced inflammation.

[Glycosaminoglycans and their fractions in patients with hereditary neuromuscular disorders].

Hereditary neuromuscular disorders (HNMD), with population incidence 1:3000, are characterized in most cases by progressive course and treatment resistance and patient's disabling. To study the involvement of the tissue-connecting structures in the pathogenesis, glycosaminoglycans and their fractions were determined in blood serum. The test group involved 40 patients with hereditary myotonia, myodystrophy, neuropathy and spinal muscular atrophy and control one consisted of 27 healthy age- and sex-matched subjects.

[The correction of post-poisoning damages to the myocardium caused by carbophos].

Lipid peroxidation processes and levels of oxidative metabolism enzymes succinate and lactate dehydrogenases, as well as morphometric parameters of cardiomyocytes were studied in rats after exposure to malathion. The efficacy of oxymethacil and atropin correction of postintoxication injuries to the myocardium was assessed. The new drug oxymethacil is recommended to be included in the treatment protocols during the first 48 h after grave poisoning with malathion.

[Post-intoxication hypothermia and processes of free radical lipid peroxidation in the rat brain and heart in organophosphorus cholinesterase inhibitor poisoning].

The activity of lipid peroxidation (LP) of the brain and myocardium as well as the intensity of the body hypothermia during 30 days after a single introduction of poisons at a dose < D50 were studied on the models of induced toxicosis in rats due to two organophosphorus compounds (malathion and armin). It has been established that the maximums of diene conjugates and Schiff's bases accumulation in the rat organs poisoned with malathion and the intensity of hypothermic response on days 14 and 21 after intoxication coincided. The similar elevated lipid peroxidation was associated with esophageal hypothermia on day 14 after armin administration.