Association between Reactogenicity and Immunogenicity in a Vaccinated Cohort with Two mRNA SARS-CoV-2 Vaccines at a High-Complexity Reference Hospital: A Analysis on Immunology Aspects of a Prospective Cohort Study.

Enhancing our comprehension of mRNA vaccines may facilitate the future design of novel vaccines aimed at augmenting immune protection while minimising reactogenic responses. Before this design is carried out, it is important to determine whether adaptive immunity correlates with the reactogenicity profile of vaccines. We studied a large cohort that was vaccinated with mRNA vaccines to answer this question.

Academic challenges on advanced therapy medicinal products' development: a regulatory perspective.

Advanced therapy medicinal products (ATMPs) are becoming the new kid on the block for the treatment of a variety of indications with promising results. Despite the academic contribution to the basic and clinical research of ATMPs, undertaking a full product development process is extraordinarily challenging and demanding for academic institutions. Meeting regulatory requirements is probably the most challenging aspect of academic development, considering the limited experience and resources compared with pharmaceutical companies.

Tolerability and Reactogenicity Profile of mRNA SARS-Cov-2 Vaccines from a Mass Vaccination Campaign in a Tertiary Hospital: Between-Vaccine and Between-Population Prospective Observational Study (VigilVacCOVID Study).

Background: The comparative safety profile of SARS-Cov2 vaccines requires further characterization in real-world settings.

Objectives: The aim of the VigilVacCOVID study was to assess the short-term safety of BNT162b2 and mRNA-1273 during the vaccination campaign of healthcare professionals (HCPs) and solid-organ transplant recipients (SOTRs) at a hospital clinic.

Methods: We conducted an observational, prospective, single-center, post-authorization study to characterize short-term adverse reactions (ARs) after vaccination.

Bilateral Leydig cell tumor in a six-year-old intersex goat affected by Polled Intersex Syndrome.

A 6-year-old, sterile, Blanca Celtibérica breed adult doe was referred to our faculty. The doe had external female genitalia, a short anogenital distance, and normally shaped udders. Masculinization signs in the head shape and male behavior were also noted at the time of referral.

Cyclodextrin effect on solvolysis of substituted benzoyl chlorides.

A kinetic study was carried out on the solvolysis of substituted benzoyl chlorides in the presence of alpha-, beta- and gamma-CD. Combination of the substituent dependent mechanism for solvolysis of benzoyl chlorides and the complexation ability of the cyclodextrin yields the following experimental behavior: (i) catalysis by beta- and gamma-CD for solvolysis of electron-attracting substituted benzoyl chlorides due to the reaction with its hydroxyl group C(6); (ii) absence of alpha-CD influence on solvolysis of benzoyl chlorides with electron withdrawing substituents; (iii) inhibition of solvolysis of benzoyl chlorides with electron-donating groups. This behavior is observed for solvolysis of meta/para substituted substrates in the presence of beta-CD, solvolysis of meta-substituted benzoyl chlorides in the presence of alpha-CD and solvolysis of para-substituted benzoyl chlorides in the presence of gamma-CD.

Identification of avian dendritic cells in the spleen using a monoclonal antibody specific for chicken follicular dendritic cells.

Background: In the chicken, circulating antigens enter the splenic white pulp via the Schweigger-Seidel sheaths (ellipsoids), where they are bound by cells, the ellipsoid associated cells (EAC), which are located on the periphery of the ellipsoid. There is an increasing body of evidence that these antigen-binding cells move through the PALS, to be finally located within the germinal centers, where these antigen-transporting EAC function as follicular dendritic cells (FDC). The aim of the current study was to further study the relationship between the EAC, the FDC, and the antigen-bearing EAC which migrate through the splenic white pulp.

Distribution of bursal secretory dendritic cells in the chicken.

Background: The bursa of Fabricius provided the microenvironment for B-cell differentiation. Continuous contact between lymphoid cells and antigen in the bursa further suggested that antigenic material has an important influence on the maintenance and development of B cells in the bursa. In addition, a dendritic cell, the bursal secretory dendritic cell (BSDC), has been identified in the medulla.

Ultrastructural identification of the splenic follicular dendritic cells in the chicken.

Background: There is a need to identify the follicular dendritic cells (FDC) of the chicken spleen at the ultrastructural level during a secondary immune response.

Methods: The cells were identified after intravenous priming BSA and boosting with biotinylated BSA conjugated to colloidal gold particles. Monoclonal antibodies raised specifically either to chicken IgG or IgM were used to characterize these immune complex-trapping cells.

Antigen-binding cells in the cecal tonsil and Peyer's patches of the chicken after bovine serum albumin administration.

It has been previously reported in the chicken that the ellipsoid-associated cells (EAC), which are considered to be a type of splenic dendritic cell, migrate from the spleen into the blood after binding antigen on their surface. In the current study we traced the localization of these cells within two peripheral lymphoid organs, the cecal tonsil (CT) and the Peyer's patches (PP). The migration of the cells was followed by light microscopy using bovine serum albumin bound to biotin and conjugated to gold particles as a histochemically identifiable antigen detected as peroxidase reaction.

Localization of splenic cells with antigen-transporting capability in the chicken.

Background: The objective of the present study is to investigate the migration pattern of the splenic dendritic cell of the chicken named the ellipsoid-associated cell (EAC) from the site of initial location at the periphery of the ellipsoid to the splenic T- and B-dependent areas.

Methods: Bovine serum albumin bound to biotin and conjugated to gold particles was used as a histochemically identifiable antigen detected as a peroxidase reaction. The antigen was intravenously injected, and subsequently its pattern of distribution in a time sequence and within the tissue was examined at the light and electron microscopy levels.

Immunocytochemical and immunoelectron microscopy study of dendritic cells in the caecal tonsils of chickens infected with Eimeria tenella.

In the present study S-100 protein containing cells in the caecal tonsil were investigated, both at light microscopic and at electron microscopic levels, after oral coccidia inoculation (Eimeria tenella). The birds were infected with a single (Day 0) or two (Days 0 and 21) infective doses of 500 oocysts. Immunoelectron reactivity for S-100 protein was demonstrated in infected chickens, but not in controls.

Fibronectin synthesis in the harderian gland of the chicken.

Immunoelectron microscopic staining with a monoclonal antibody against fibronectin demonstrated the presence of this extracellular matrix glycoprotein in the avian Harderian gland. Fibronectin was detected as a component of the electron-dense material, which has been observed both between the epithelial cells lining the ducts of the gland and between the lymphoid cells within the subepithelial lymphoid tissue. Additionally, intracellular fibronectin was detected in the rough endoplasmic reticulum, Golgi complex, and secretory vacuoles in the cytoplasm of a cell, showing the ultrastructural features of a myofibroblast.

Follicular dendritic cell activation in the harderian gland of the chicken.

The avian follicular dendritic cell changes that occur in the germinal center of the Harderian gland during the course of the immune response were studied by electron microscopy and the immunoperoxidase method was employed for the detection of S-100 protein. The chickens were injected twice with Salmonella O Antigen into the nictitating membrane at 9-day intervals. The follicular dendritic cells exhibited filiform processes at between 24 and 96 h after the second antigen administration.

Quantitative assessment of ATPase-positive epidermal dendritic (Langerhans) cells in piglets and adult swine.

To assess the distribution pattern of Langerhans cells (LC) in normal porcine skin, epidermal sheets from six anatomical sites from three age-matched groups of male and female pigs were stained for ATPase activity. This histoenzymological technique is considered specific for Langerhans cells in normal epidermis. No statistically significant differences were observed between mean Langerhans cell density per mm2 of epidermis from male and female pigs, nor between different anatomical sites in the same age group.

Early in-vitro histological chondral differentiation.

In vitro chondrogenesis is possible in the chick embryo from stage 4 of Hamburger and Hamilton (1951), only 18-19 hours of incubation, before somite formation. In stage 4 of Hamburger and Hamilton (1951) the chondroblasts are placed laterally to the primitive streak and notochord cells are not necessary for cartilage differentiation.

Ultrastructural localization of a soluble antigen in the chicken Harderian gland.

The relationship between plasma cells, macrophages, B and T cells, dendritic cells, and epithelium in the chicken Harderian gland have been studied by means of ultrastructural localization of the horseradish peroxidase following local immunization. After 5 d, peroxidase activity was found in vesicles located in macrophages and immature plasma cells. On day 7, peroxidase-antiperoxidase complexes were found in vesicles of the epithelial cells lining the secondary ducts and the acini, in the lumina of the ducts, and on the surface of lymphocytes located among these epithelial cells.

Immunocytochemical detection of dendritic cell by S-100 protein in the chicken.

Positivity for S-100 protein in paraffin embedded chicken lymphoid tissue was found by using a polyclonal antibody against whole bovine S-100 protein. The S-100 protein-containing cells were observed in the locations which have been reported to contain avian dendritic cells such as the medulla of the bursal follicles, and the germinal centers and T-dependent areas in the spleen, Peyer's patches, caecal tonsil and Harderian gland. Positive cells were also found in the location where ellipsoid associated cell have been described, and between epithelial cells covering the Peyer's patches and the caecal tonsil, as well as between the cells lining the ducts within the Harderian gland.

Immunoglobulin classes synthesised by the chicken Harderian gland after local immunisation.

The immunoglobulin (Ig) levels in tears and sera were compared after antigen administration (salmonella O antigen) by eyedrop and injection into the nictitating membrane, to determine the Ig classes synthesised by the plasma cells in the chicken Harderian gland. Samples of tears and sera were collected from immunised and control birds between 24 hours and 24 days after the antigen or sterile saline was administered. Samples were assayed for IgA, IgG and IgM concentrations using radial immunodiffusion.

Local immune response in the chicken Harderian gland to antigen given by different ocular routes.

The effectiveness of three ocular routes of antigen administration to produce a local immune response in the Harderian gland was studied. The routes were by eyedrop, injection into the ocular conjunctiva and injection into the nictitating membrane. The antigen was observed in the cytoplasm of macrophages located within the lymphoid tissue only after the injection into the nictitating membrane.

[Laryngeal cancer in females. Considerations on a juvenile case].

The authors commented a case of carcinoma of the larynx in a young non-smoking woman with antecedents of repetitive endolaryngeal traumatisms. The familial and genetic factors were also considered.

Myofibroblasts and myoepithelial cells in the chicken harderian gland.

An electron microscopic study of the myoepithelial cells in the chicken Harderian gland provides evidence that these cells can be transformed into myofibroblasts. After the application of a Brucella ovis suspension in sterile saline onto the eyeball, every 5 minutes for half an hour, myoepithelial cells gradually develop over a 90-minute period the characteristic features of myofibroblasts: bundles of intracytoplasmic microfilament; abundant rough endoplasmic reticulum; prominent Golgi complex; and surface membrane differentiations, that provide attachment to neighbouring epithelial cells. No typical desmosomes are observed.

Granulopoiesis in the pineal gland of chickens.

Foci of differentiating heterophilic granulocytes in the pineal gland were studied by light and electron microscopy in chickens, from hatching until 56 weeks of age. Foci of granulopoiesis could be seen in the first 24 hours after hatching. Thereafter, their number and cellular density increased, becoming highest at 2 weeks.

Results and histological development of various surgical techniques for correcting eversion of the third eyelid in dogs.

An experimental study was made of 20 dogs in order to compare various surgical techniques used to correct eversion of the third eyelid, namely resection of most of the cartilage, resection of the central portion of the cartilage, and cartilage homotransplantation. An analysis was made of histological results obtained 45 days after operation, the most satisfactory result being recorded for homotransplantation of the third eyelid cartilage.

Histopathological changes in ewe lambs exposed to prolonged diet on lucerne.

Histopathological changes were observed in 10 ewe lambs reared during nine months on lucerne as compared with 10 controls reared on ryegrass. These changes consisted of periglandular oedema and subacute endometritis in the uterus, secretory-cell hyperplasia with prevalence of neutral mucopolysaccharides in the cervix and hyperkeratinisation and colpitis in the vagina. The mammary glands of lambs reared on lucerne were significantly heavier and showed secretory activity.