Exposure to laser light can negatively affect the examination of cell cultures with holographic imaging techniques, leading researchers to seek alternatives like using lower intensity and broader spectrum light.
The study focuses on lensless digital holographic microscopy (LDHM) operating under low photon budget (LPB) conditions, which allows for effective imaging of live cells while minimizing sample interaction.
Results show that even with low illumination power (down to 7 µW), the quality of holographic imaging remains high, supported by numerical denoising techniques, and successfully demonstrated on complex biological samples like live glial restricted progenitor cells.
Laser-based lensless digital holographic microscopy (LDHM) is often spoiled by considerable coherent noise factor. We propose a novel LDHM method with significantly limited coherent artifacts, e.g.