Publications by authors named "Bartosz Majewski"
Article Synopsis
- - The study investigates the potential of chemically modified in vitro transcribed (IVT) mRNAs to enhance their stability and efficiency for applications like gene therapy and cancer treatment, especially focusing on the significance of the mRNA 5'-cap structure in regulating translation.
- - Researchers developed 16 new cap analogs by combining a trinucleotide IVT capping technology with various modifications to the 5' cap and added epigenetic marks that are similar to those found in the mRNA of more complex organisms.
- - The effects of these new analogs were tested on their interaction with a key protein involved in translation, as well as their translation efficiency in cell models, with one promising modification further evaluated in a mouse model to assess
Trinucleotide mRNA Cap Analogue 6-Benzylated at the Site of Posttranscriptional A Mark Facilitates mRNA Purification and Confers Superior Translational Properties In Vitro and In Vivo.
Marcin Warminski Edyta Trepkowska Miroslaw Smietanski Pawel J Sikorski Marek R Baranowski Bartosz Majewski
J Am Chem Soc
March 2024
Eukaryotic mRNAs undergo cotranscriptional 5'-end modification with a 7-methylguanosine cap. In higher eukaryotes, the cap carries additional methylations, such as A─a common epitranscriptomic mark unique to the mRNA 5'-end. This modification is regulated by the Pcif1 methyltransferase and the FTO demethylase, but its biological function is still unknown.
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