Evaluation of the biological effects of police radar RAMER 7F.

This paper presents results of experiments on the effects of electromagnetic radiation in the millimeter range (frequency 34.0 +/- 0.1 GHz, power density 20 muW/cm2) emitted by a police radar device.

Elevation of extracellular adenosine induces radioprotective effects in mice.

The radioprotective effectiveness of the elevation of extracellular adenosine induced in mice by the combined administration of dipyridamole, a drug inhibiting the cellular uptake of adenosine, and adenosine monophosphate, a soluble adenosine pro-drug, was evaluated. Based on survival studies, endogenous hemopoietic spleen colony formation, and the postirradiation behavior of bone marrow granulocyte-macrophage colony-forming cells (GM-CFC), it was demonstrated that the combined administration of dipyridamole and AMP protects mice when given either 15 or 60 min before irradiation. It could be deduced that the radioprotective action is induced by at least two independent mechanisms: (1) protection by hypoxia as a result of the effect of the treatment on the cardiovascular system, and (2) and enhanced regeneration of the hemopoietic stem cells due to either enhanced postirradiation repair or an increased proliferation of the hemopoietic stem cells.

Hematopoietic recovery in repeatedly irradiated mice can be enhanced by a repeatedly administered combination of diclofenac and glucan.

A combination of diclofenac and glucan administered repeatedly in a protective regimen in the course of repeated gamma irradiation of mice (6 x 2 Gy during 3 weeks) enhanced granulopoiesis and other indices of hematopoietic recovery investigated from 3 to 7 days after the last radiation exposure. Repeated administration of diclofenac or glucan alone or treatment of the mice with the diclofenac-glucan combination given once before the first or the last radiation exposure did not induce such effects. The protective effect of the repeatedly administered combination of the drugs was realized despite the fact that the response of the serum colony-stimulating activity to the repeated combined drug administration was decreased at the end of the treatment regimen compared to that of mice given this drug combination only once.

Effects of microwaves on membranes of hematopoietic cells in their structural and functional organization.

The role of cell membranes in stimulating and inhibiting the effects of microwaves was investigated in experiments carried out with a suspension of murine bone marrow cells irradiated with microwaves in vitro [f = 2.45 GHz, CW, specific absorption rate (SAR) = 12 W/kg]. Results obtained by means of a structural probe, 2.

[The effect of gas hypoxia (10% O2) and dextran sulfate on the radiosensitivity of the stem cells of the intestinal epithelium in mice].

Injection of dextran sulphate before irradiation was shown to protect jejunal epithelium stem cells (D0 increased from 1.13 to 1.82 Gy).

CFU-S and haematopoietic microenvironment in mice after fractionated irradiation. A study on spleen colonies.

The paper is aimed at evaluating the quantity and quality of the haematopoietic stem cells, CFU-S, in the bone marrow and the functional effectiveness of the haematopoietic microenvironment of the spleen in two time intervals after repeated exposure of mice to doses of 0.5 Gy gamma-rays once a week (total doses of 12 and 24 Gy). After irradiation, bone marrow was cross-transplanted between fractionatedly irradiated and control mice.

Effect of dipyridamole and adenosine monophosphate on cell proliferation in the hemopoietic tissue of normal and gamma-irradiated mice.

Combined treatment with dipyridamole and adenosine monophosphate enhances cell proliferation in the hemopoietic tissue of normal and gamma-irradiated mice. This effect can be explained by the elevation of extracellular adenosine, and the receptor-mediated activation of the cell adenylate cyclase system.

Amelioration of radiation damage to haemopoiesis by Ivastimul, given after irradiation to mice protected by peroral cystamine.

The combined radioprotection by preirradiation peroral cystamine and postirradiation Ivastimul treatment was examined in sublethally and lethally whole-body gamma-irradiated mice. The enhancement of haemopoietic recovery and increased survival of irradiated mice was demonstrated when a single dose of Ivastimul was administered after irradiation. Ameliorative influence of combined radioprotection may be explained by the haemopoietic stem cell protection by cystamine and haemopoietic stimulation mediated by Ivastimul.

Decrease in the number of progenitors of erythrocytes (BFUe, CFUe), granulocytes and macrophages (GM-CFC) in bone marrow of rats after a 14-day flight onboard the Cosmos-2044 Biosatellite.

A decrease in the number of progenitors of erythrocytes (BFUe, CFUe) and of granulocytes and macrophages (GM-CFC) in bone marrow was found in rats exposed to microgravitation during a 14-day flight onboard the Cosmos-2044 biosatellite when compared to control rats maintained under conditions of gravitation on the ground. The number of progenitors of both lineages of haemopoiesis was also decreased in synchronous control rats, thus suggesting that the pool of progenitors is influenced also by the action of the nonspecific space flight factors.

Radioprotective effect of dextran sulphate and aerogenic hypoxia on intestinal crypt stem cells in mice.

A single intraperitoneal injection of dextran sulphate given 6 h before irradiation produced higher numbers of microcolonies of intestinal crypt stem cells in whole-body irradiated mice than injection of saline in control mice. If dextran sulphate and hypoxia are combined, the radioprotective effect of hypoxia on intestinal crypt stem cells depends on the time interval between irradiation and administration of dextran sulphate.

Radioprotection of hemopoiesis conferred by aqueous extract from chlorococcal algae (Ivastimul) administered to mice before irradiation.

In experiments on mice, single i.p. injections of aqueous extract from chlorococcal freshwater algae (Ivastimul) were found to increase their radioresistance.

Increase in the capacity of bone marrow exposed to He-Ne laser radiation for growth of GM-CFC colonies in vitro.

Single exposure of murine bone marrow cell suspension to He-Ne laser radiation elevates the potential of haemopoietic stem cells for growth of GM-CFC colonies in vitro. The stimulatory action of He-Ne laser persists for 60 min after short-term irradiation.

Decrease in the number of progenitors of fibroblasts (CFUf) in bone marrow of rats after a 14-day flight onboard the Cosmos-2044 biosatellite.

A decrease in the number of progenitors of fibroblasts (CFUf) was found in bone marrow of rats that underwent a 14-day flight in the state of weightlessness onboard the Cosmos-2044 biosatellite, immediately after flight, when compared with rats maintained in control conditions of terrestrial gravitation. These changes may be explained by the action of specific factors of microgravitation.

Hydroxyurea as a suppressor of the radioprotective effect of dextran sulfate on bone marrow hemopoietic stem cells in mice.

The changes in hemopoiesis of mice following intraperitoneal administration of dextran sulfate (DS) 24 h before irradiation, as well as the effect of injecting them with hydroxyurea (HU), were analyzed. DS increased the proliferation activity and number of hemopoietic stem cells (CFUs) in the bone marrow. The content of CFUs in the bone marrow and the number of endogenous spleen colonies (ESC) of hemopoietic tissue were higher after irradiation in mice treated with DS than in control groups.

The radioprotective effects of aqueous extract from chlorococcal freshwater algae (Chlorella kessleri) in mice and rats.

In experiments on mice and rats the effect was studied of a single administration of aqueous extract from chlorococcal freshwater algae - Ivastimul - on their radiosensitivity. After injection of this substance the number of haemopoietic stem cells in the bone marrow and spleen of mice (CFUs) increased, as did their survival after irradiation. Irradiation with a lethal dose of gamma rays 24 hours after the injection of Ivastimul is survived by a larger number of treated mice and rats than untreated ones.

Injection of dextran sulphate enhances in mice the stimulatory influence of serum on growth of haemopoietic tissue colonies (GM-CFC) in vitro.

A single i.p. injection of dextran sulphate elevates in mice the activity of blood serum for the stimulation of haemopoietic stem cell (GM-CFC) growth in vitro.

[Enhancement of the radioprotective action of gas-induced hypoxia by using dextran sulfate].

Mice of the strain (CBA X C57Bl) F1 injected with a single dose (40 mg.kg-1) of dextran sulphate (m.w.

Effect of isoprinosine on haemopoietic stem cells (CFU) in bone marrow and spleen of mice.

Peroral application of Isoprinosine for 3, 5 and 10 days increases the number and proliferative activity of haemopoietic stem cells (CFUs) in the bone marrow of mice. Changes in the spleen occur later and are manifested by an increase in CFUs proliferative activity and by a transient decrease in CFUs number.

Effects of microwaves on the colony-forming capacity of haemopoietic stem cells in mice.

A suspension of bone marrow cells from femurs of female (CBA X C57Bl)F1 mice was exposed to 2450 MHz CW microwaves in a specially designed waveguide exposure system. The temperature of the suspension rose, during exposure to microwaves, from 20 degrees C to 45 degrees C, and at an interval within 20 degrees C to 45 degrees C the number of haemopoietic stem cells (CFUs) was determined by the spleen exocolony method. The time of exposure of bone marrow cells to each temperature studied was 20 s.

Increased protective effect of hypoxia against radiation following activation of haemopoiesis with dextran sulphate.

The effect of pretreatment of mice with a single injection of dextran sulphate (DS) on the protective capacity of moderate hypoxia was analysed. Dextran sulphate activated haemopoietic stem cell populations, and irradiation under hypoxia one day after DS injection increased the number of haemopoietic stem cells surviving in the bone marrow of the femur, their recovery after irradiation, and the number of endogenous spleen colonies. A moderate hypoxia (15% O2 and 12% O2) significantly reduced the lethal effect of gamma rays in mice after DS injection and increased the value of LD 50/30.

Increased protective effect of cystamine and mexamine against radiation after activation of the haemopoiesis by dextran sulphate in mice.

The effect gained by activation of haemopoietic cell populations by a single injection of dextran sulphate (DS) on the protective effect of cystamine and mexamine administered in small doses was monitored in mice. On irradiation 24 hours after the injection of DS the number of endogenous spleen colonies increased following the administration of mexamine and cystamine, the number of haemopoietic stem cells surviving in the femur after irradiation increased, and recovery of the cellularity of irradiated bone marrow was accelerated. Both substances administered 24 hours after the injection of DS significantly reduced the lethal effects of gamma rays.

Changes in the number of haemopoietic stem cells (CFUs) in bone marrow and spleens of pregnant rats after a short space flight onboard the Cosmos-1514 biosatellite.

After a 5-day stay in a state of weightlessness onboard a biosatellite Cosmos-1514 the pregnant rats exhibited a decrease in the number of haemopoietic stem cells (CFUs) in bone marrow and spleens on the recovery day after the space flight as compared to rats kept in the vivarium under usual conditions. The different changes in the concentration of CFUs in the bone marrow and spleens of flight and synchronous control rats indicate that the extent of the decrease in CFUs pool in the spleens of flight rats was significantly influenced by the action of non-specific flight factors.

[Effect of a physiological solution on the hematopoietic stem cell pool in healthy and irradiated mice].

It was shown on (CBA X C57Bl)F1 mice that a single intraperitoneal injection of a physiological solution (0.2 ml) reduced the number of haemopoietic stem cells in the bone marrow and increased concurrently the radioresistance of CFU.