hEIDI: An Intuitive Application Tool To Organize and Treat Large-Scale Proteomics Data.

Advances in high-throughput proteomics have led to a rapid increase in the number, size, and complexity of the associated data sets. Managing and extracting reliable information from such large series of data sets require the use of dedicated software organized in a consistent pipeline to reduce, validate, exploit, and ultimately export data. The compilation of multiple mass-spectrometry-based identification and quantification results obtained in the context of a large-scale project represents a real challenge for developers of bioinformatics solutions.

"Cri-du-chat" syndrome in a patient born to a mother with a paracentric inversion of chromosome 5q.

We report the case of a female child presented at birth with hypotonia, growth retardation and respiratory distress. Chromosome study from peripheral blood showed a 46,XX,del(5)(p14pter) karyotype. Parental chromosome studies revealed that the mother carried an apparently balanced paracentric inversion of long arms of one chromosome 5, giving the karyotype 46,XX,inv(5)(q12q32), whereas paternal karyotype was normal.

Urethral cytology and diagnosis of the pregnancy term. About some observations on the use of this method in an hospital setting.

A retrospective study of routine urethrocytograms performed over a 10-year period in 3,478 pregnant women for the diagnosis of pregnancy term was carried out to determine the reliability of urethral cytology when two, three, or four successive urethral smears were performed at 2- or 3-day intervals, and to investigate the correlations between the cytological results and the neurological ages of newborns. Microscopical examination of the 3,478 routine smears demonstrated the existence of a before-term aspect, a near-term aspect, or an at-term aspect in 7.4%, 66.

Identical chromosome imbalance in two siblings born to a mother with a double reciprocal translocation.

We report the case of a woman who carried two reciprocal translocations. Her karyotype was 46,XX,t(3;12)(q12;q21)(4;17)(p14;p13). She had two children, a phenotypically normal daughter (karyotype (46,XX,t(3;12)(q12;q21)) and a son with partial 4p trisomy (karyotype 46,XY,t(3;12) (q12;q21),-17,+maternal der(17)).

Phosphorylated neurofilament expression and resistance to kainate toxicity.

Antibodies directed against phosphorylated neurofilaments, which are major proteins of the neuronal cytoskeleton, usually do not label neuronal cell bodies except in some neurological diseases. In the present study, we show that in rat cortical cell cultures exposed to kainate there is an inverse relation between neuronal survival and the proportion of neuronal cell bodies stained by a monoclonal antibody (clone SMI31) that recognizes extensively phosphorylated neurofilament proteins (150 kDa and 200 kDa). The immunoblot analysis also revealed an increase in 150-kDa phosphorylated neurofilament expression in kainate-treated cell cultures.

Synaptophysin expression during in vitro neuronal differentiation. An immunocytochemical and a confocal laser microscopic study.

Primary neuronal cultures from mammalian fetal brains are widely used for morphological, biochemical and pharmacotoxicological studies. The usefulness of relatively pure neuronal cultures are now demonstrated for such studies. We have compared the neuronal survival and differentiation, the synaptophysin expression and the glial cell percentage in primary neuronal cultures using two different media: a M1 medium containing 10 % fetal calf serum and a M2 medium supplemented with hormones, ions and chemicals.

A dose-dependent increase of Tau immunostaining is produced by glutamate toxicity in primary neuronal cultures.

Neuronal degeneration was produced in primary cultures by glutamate exposure and the modifications of Tau immunoreactivity were analysed in degenerating neurons. After 8-12 days of culture, glutamate was applied at different concentrations (50, 100, 200 and 500 mumol) in a Na(+)- and Mg(2+)-free solution containing calcium. Prior to and 12 hours after glutamate exposure cell death was defined by cell counting in each dish.

[Epidemiological observations on iridovirosis of Lymnaea truncatula, host mollusca of Fasciola hepatica].

Epidemiological studies were carried out in 11 populations of Lymnaea truncatula in 1983-1984 and 1987-1989 to determine the prevalence of snail infection by an iridovirus. The virus was found in the different populations and samples, with a frequency ranging from 1.6 to 87%.

[Stratified cylindrical epithelium of the human urethra. Apropos of various cytological observations on urocytograms].

The stratified columnar cells of the human urethra were studied by the filtering membrane technique in urocytograms usually effected, or in smears made after a cytoscopy or after an ejaculation. The cells were present in 61% of systematic filters without inflammation, in 82% of inflammatory filters, and in the urine of 90% of persons after a cytoscopy. The mean number of columnar cells did not exceed 1% in usual filters without inflammation; the percentage varied with the donor sex, but it did not show a significant variation with the donor age.

[The effect and consequences of inflammation on urethral cytology in the 9th month of pregnancy].

Qualitative and quantitative modifications were observed in urethral smears made from week 37 to week 41 of amenorrhoea and showing leucocytes in the desquamation. The number of cell clusters decreased; the numbers of false clusters and of isolated cells with rounded borders increased. Columnar cells and degenerating isolated nuclei are often seen in these inflammatory smears.

[Urethral cytology and diagnosis of the term of pregnancy. Changes in the morphological aspects during the last trimester].

Urethrocytograms were made by brushing in women during the last quarter of their pregnancy, from week 28 of amenorrhea to week 38. Two different cell patterns were present in these smears. The A-pattern is composed by round squamous cells, often in clumps.

Kainic acid induces early and delayed degenerative neuronal changes in rat spinal cord.

Intrathecal injections of kainic acid and sodium chloride were performed in rats to study the cellular modifications observed in spinal cord. Early neuronal changes (2 and 24 h) associated dark and shrunken or swollen and vacuolated cytoplasms. Delayed (3, 6, 14 days) changes, mainly consisted in degenerative aspect of motoneurons with eccentric and indented nucleus, swollen cytoplasms with proximal neurite enlargements, the presence of 'lipofuscin-like' pigments, disorganized intracytoplasmic organelles and filament accumulations.

[Fasciola hepatica L.: the productivity of a sporocyst as a function of the size of Lymnaea truncatula Müller].

Experimental studies were undertaken on the rediae of Fasciola hepatica and on their content in two populations of Lymnaea truncatula snails, individually exposed to a single miracidium and fixed for histologic observations just after their death between days 74 and 81 postexposure at 20 degrees C. The numbers of free rediae and of larval stages per snail increased with the shell height. A constant number of free rediae was observed in snails measuring 11 mm and more in size.

[Transmission of an 18 ring chromosome in two generations in subjects of normal phenotype].

We report the clinical and cytogenetic findings on three cases with ring chromosome 18. These patients are phenotypically apparently normal. The 18 ring chromosome was segregating in two generations.

[Histopathological studies in young Lymnae glabra Müller of live or of abortive infestation].

Very young snails had been individually exposed to a single miracidium of Fasciola hepatica; they were bred at 23 degrees C during 49 days and killed at days 42 and 49 postexposure for histological studies. Tissue lesions were observed in six organs of infected snails and none in uninfected and control snails. All snails with living parthenitae showed a necrotized and atrophic gonad; a reconstitution with epithelial hyperplasia was noted in the most digestive glands, kidneys and genital annex glands of these snails.

[Intercalary de novo deletion of chromosome 1: del(1) (q24 to q32)].

We present one unrelated girl with a de novo interstitial deletion of a segment in the long arm of chromosome 1 (q24----q32). Comparison of the phenotypic characteristics of this proband with those of six previously described patients with similar deletion, does not suggest the existence of a 1q interstitial deletion syndrome. Clinical manifestations of these patients are variable and non specific: intrauterine growth retardation, low set ears, height and weight failure and mental retardation, clinodactyly of the fifth fingers.