Outcome satisfaction in long-term survivors of oncologic limb salvage procedures.

Objective: We present data from our recently completed study that suggests that joint salvage is the key parameter in keeping the limb salvage ethos relevant.

Methods: We reviewed all patients operated on nationally over 30 years (1978 to 2008). We identified 265 survivors of which 162 were available for evaluation at the time of survey.

An autologous cell lysate extract from human embryonic stem cell (hESC) derived osteoblasts can enhance osteogenesis of hESC.

It was recently demonstrated that osteogenesis of hESC was more efficient without the initial embryoid body formation step. This study sought to further improve this direct differentiation culture system, by developing an autologous osteogenic-inducing culture supplement extracted from hESC-derived osteogenic cells themselves. A whole cell lysate was prepared from hESC-derived osteogenic cells, simply by exposure to deionized water followed by free-thawing and subsequent filtration.