Binding interaction of differently charged fluorescent probes with egg yolk phosphatidylcholine and the effect of β-cyclodextrin on the lipid-probe complexes: a fluorometric investigation.

Interaction of cationic phenosafranin (PSF), anionic 8-anilino-1-naphthalene sulfonate (ANS) and non-ionic nile red (NR) have been studied with the zwitterionic phospholipid, egg yolk L-α-phosphatidylcholine (EYPC). The study reveals discernible binding interactions of the three fluorescent probes with the EYPC lipid vesicle. Once the binding of the probes with the lipid is established, the effect of cyclic oligosaccharide, β-cyclodextrin (β-CD), on these lipid bound probes has been investigated.

Competitive binding of nile red between lipids and β-cyclodextrin.

Fluorometric measurements are exploited to explore the binding interactions of nile red (NR) with anionic lipid dimyristoyl-L-α-phosphatidylglycerol (DMPG), zwitterionic lipid dimyristoyl-L-α-phosphatidylcholine (DMPC) as well as neutral cyclic oligosaccharide β-cyclodextrin (β-CD) solutions. The binding constants are found to be quite high and comparable (within a factor of five). Series of spectral techniques like steady state fluorescence and fluorescence anisotropy study, micropolarity study, quenching study and time resolved experiments reveal that the addition of β-CD to the probe-lipid complexes results in weakening of the lipid-probe interaction and formation of probe-β-CD inclusion complexes leading to the removal of some of the probe (NR) molecules from the lipid environments.

Multiple emissions of α-naphthil: fluorescence from S2 state.

Multiple emissions of α-naphthil are reported in solution phase at room temperature as well as in ethanol glassy matrix at 77 K, frozen under different conditions of illumination. Steady state and time-resolved fluorometric measurements are undertaken to assign the observed emissions to the different species in terms of geometry and electronic states of the fluorophore. The unexplored emission band peaking at ∼423 nm has been ascribed to originate from the S2 electronic state.

Spectroscopic exploration of mode of binding of ctDNA with 3-hydroxyflavone: a contrast to the mode of binding with flavonoids having additional hydroxyl groups.

Binding interaction of 3-hydroxyflavone (3HF), a bioactive flavonoid, with calf-thymus DNA (ctDNA) has been explored exploiting various experimental techniques. The dual fluorescence of 3HF resulting from the excited state intramolecular proton transfer (ESIPT) is modified remarkably upon binding with the biomacromolecule. The determined binding constant, fluorescence quenching experiment, circular dichroism (CD) study, comparative binding study with the known intercalative binder ethidium bromide and thermometric experiment relating to the helix melting of ctDNA confirm the groove binding of 3HF with the DNA.

Multiple emissions of benzil at room temperature and 77 K and their assignments from ab initio quantum chemical calculations.

Multiple emissions have been observed from benzil under different conditions in solutions at room temperature as well as in low temperature glass matrices at 77 K. Low temperature emission has been monitored in rigid matrices frozen under different conditions of illumination. Steady state and time-resolved results together with the ab initio quantum chemical calculations provide, for the first time, the assignments of the different fluorescence bands to the different geometries and∕or electronic states of the fluorophore molecule.