On the coupling of mechanics with bioelectricity and its role in morphogenesis.

The role of endogenous bioelectricity in morphogenesis has recently been explored through the finite volume-based code . We extend this platform to electrostatic and osmotic forces due to bioelectrical ion fluxes, causing cell cluster deformation. We further account for mechanosensitive ion channels, which, gated by membrane tension, modulate ion fluxes and, ultimately, bioelectrical forces.

Arthrogenic human synovial cysts: immunohistochemical profile of interleukin-1beta, interleukin-6, tumour necrosis factor-alpha.

Background: Synovial cysts are currently classified as degenerative lesions affecting the joint capsule or adjacent structures.

Materials And Methods: In our study we describe the results obtained in an immunohistochemical study comprising 18 patients with synovial cysts, performed to evaluate the pathophysiological role of some inflammatory cytokines such as: interleukin (IL)-1β, IL-6 and tumour necrosis factor-alpha (TNF-α).

Results: Results showed an over-expression of TNF-α, IL-1β and IL-6 which appears to be involved in the onset and progression of the disease.

Short-term testicular warming under anesthesia causes similar increases in testicular blood flow in Bos taurus versus Bos indicus bulls, but no apparent hypoxia.

Bull testes must be 4-5 °C below body temperature, with testicular warming more likely to cause poor-quality sperm in Bos taurus (European/British) versus Bos indicus (Indian/zebu) bulls. Despite a long-standing dogma that testicular hyperthermia causes hypoxia, we reported that increasing testicular temperature in bulls and rams enhanced testicular blood flow and O delivery/uptake, without hypoxia. Our objective was to determine effects of short-term testicular hyperthermia on testicular blood flow, O delivery and uptake and evidence of testicular hypoxia in pubertal Angus (B.

A potential for higher-order phenomenological strain gradient plasticity to predict reliable response under non-proportional loading.

We propose a plastic potential for higher-order (HO) phenomenological strain gradient plasticity (SGP), predicting reliable size-dependent response for general loading histories. By constructing the free energy density as a sum of quadratic plastic strain gradient contributions that each transitions into linear terms at different threshold values, we show that we can predict the expected micron-scale behaviour, including increase of strain hardening and strengthening-like behaviour with diminishing size. Furthermore, the anomalous behaviour predicted by most HO theories under non-proportional loading is avoided.

Anatomical variations of the median nerve and of the vascular-nervous structures at the wrist.

Three cases of anatomical variation of the median nerve at the wrist found during our surgical activity led us to take the opportunity to expose anatomical variations by reviewing already published reviews. Consequently, on the basis of anatomical studies, clinical reports and imaging, as a result of careful examination of the published literature, it has been observed that the interventions in such anatomical area must take into account these variations. In particular, the most performed procedure is the lysis of the transverse carpal ligament (TCL), which is not free from complications.

Trophic and neurotrophic factors in human pituitary adenomas (Review).

The pituitary gland is an organ that functionally connects the hypothalamus with the peripheral organs. The pituitary gland is an important regulator of body homeostasis during development, stress, and other processes. Pituitary adenomas are a group of tumors arising from the pituitary gland: they may be subdivided in functional or non-functional, depending on their hormonal activity.

Involvement of pro-inflammatory cytokines and growth factors in the pathogenesis of Dupuytren's contracture: a novel target for a possible future therapeutic strategy?

Dupuytren's contracture (DC) is a benign fibro-proliferative disease of the hand causing fibrotic nodules and fascial cords which determine debilitating contracture and deformities of fingers and hands. The present study was designed to characterize pro-inflammatory cytokines and growth factors involved in the pathogenesis, progression and recurrence of this disease, in order to find novel targets for alternative therapies and strategies in controlling DC. The expression of pro-inflammatory cytokines and of growth factors was detected by immunohistochemistry in fibrotic nodules and normal palmar fascia resected respectively from patients affected by DC and carpal tunnel syndrome (CTS; as negative controls).

Combination of cetuximab with chemoradiation, trastuzumab or MAPK inhibitors: mechanisms of sensitisation of cervical cancer cells.

Background: Cervical cancer (CC) annually kills 288,000 women worldwide. Unfortunately, responses to chemoradiation are partial and are of short duration. As anti-EGFR monoclonal antibodies sensitise tumours, we investigated cetuximab's toxicity plus chemoradiation on CC cells, which express different EGFR levels.

Effects of a physical education program on children's attitudes and emotions associated with sport climbing.

This study examined the effects of climbing ladders, wall bars, perches, and ropes in changing students' attitudes and emotions associated with sport climbing. The tasks were part of a physical education instructional curriculum of primary schools in Italy. Boys and girls (N = 80), ages 10 to 11 years, were involved in a 10-lesson intervention during their curricular lesson.

Mechanisms of interleukin-6 protection against ischemia-reperfusion injury in rat liver.

Numerous animal studies simulating liver injury have demonstrated that interleukin-6 (IL-6) exerts a protective effect. This study was designed to further analyze the molecular mechanisms underlying the protective role of IL-6 in a rat model of liver ischemia/reperfusion injury. We show that IL-6: (i) at high doses reduces cell damage which occurs in ischemic-reperfused liver, while at low doses displays only a limited protective capacity, (ii) anticipates and enhances hepatocyte compensatory proliferation seen in ischemic-reperfused liver also at a low, more pharmacologically acceptable dose, (iii) sustains the acute phase response which is dampened in ischemic-reperfused liver, (iv) strengthens the heat shock-stress response shown by ischemic-reperfused liver and (v) overcomes the dysfunctions of the unfolding protein response found in ischemic-reperfused liver.

The impermeant ion methylammonium blocks K+ and NH4+ currents through KAT1 channel differently: evidence for ion interaction in channel permeation.

The permeation properties of KAT1, an inward rectifying potassium channel from plant cells, were investigated with different ions in the external medium. With either K+, NH4+ or methylammonium (MA) in the external solution, the channel, expressed in Xenopus oocytes, appeared permeable to K+ and, to a lesser extent, to NH4+ but not to the slightly bigger, methylated analogue of NH4+, MA. Substituting NH4+ for K+ shifted the voltage dependency of channel activation further negative and hastened activation kinetics.

Functional characterization of a human DNase-like protein encoded by a gene positioned in Xq28.

Xib, a gene recently reported to reside on the q28 region of the human X chromosome [Pergolizzi et al. (1996) Gene 168, 267-270], contains an open reading frame homologous to those of the DNase I family enzymes. The full open reading frame of this gene has been fused to the E.

Polyamine acetylations in normal and neoplastic growth processes.

The expression patterns of cytosolic and nuclear polyamine acetyltransferases were studied in normal and neoplastic growth processesin vivo andin vitro to evidentiate the roles played by these enzymes in cell proliferation. In regenerating liver, cytosolic spermidine/spermine N(1)-acetyltransferase showed similar augments of mRNA level and enzymatic activity during the prereplicative period (4-8 h), whereas spermidine N(8)-acetyltransferase activity increased later (24 h) when DNA synthesis was maximally enhanced. In fibroblasts continuously dividing, the messenger for spermidine/spermine N(1)-acetyltransferase rapidly accumulated after serum-stimulation.

Expression patterns of ornithine decarboxylase and c-met in growing Yoshida AH-130 hepatoma.

The expression of the two proto-oncogenes ornithine decarboxylase and c-met was examined during various phases of growth of Yoshida AH-130 ascites hepatoma. Ornithine decarboxylase (ODC) and c-met mRNA levels declined progressively from day 5 (exponential growth-phase) until day 14 (quasi-stationary growth-phase). Transcription rate for both the genes remained constant between days 5 and 10, while decreasing at day 14.

Differential expression of c-jun, c-fos and hsp 70 mRNAs after folic acid and ischemia-reperfusion injury: effect of antioxidant treatment.

The regenerative repair response to folic acid and ischemia-reperfusion injury is characterized by different patterns of renal tubular cell proliferation. The purpose of this study was to examine the time course of the expression of two early growth response genes, c-jun and c-fos, and of the stress response gene hsp70 after such renal injuries and to determine the role played by reactive oxygen species generated during reperfusion, on gene induction. Ischemic injury caused an almost immediate increase of c-jun, c-fos and hsp70 mRNA expression, that reached a maximum at 1 h of reperfusion.

Expression of spermidine/spermine N1-acetyltransferase in growing Yoshida AH-130 hepatoma cells.

Activity and messenger RNA levels of spermidine/spermine N1-acetyltransferase, the rate-limiting enzyme of the polyamine interconversion pathway, were investigated in host liver and in Yoshida AH-130 ascites hepatoma cells as a function of tumor growth phases. Enzyme activity reached maximal values at day 10 in host liver (2.0-fold increase) and at days 10 and 14 in hepatoma cells (4.

Prolonged stabilization of multiple and single brain metastases from breast cancer with tamoxifen. Report of three cases.

Cancer frequently metastasizes to the brain, and such lesions, whether multiple or solitary, have a poor prognosis, despite all efforts to treat them. There have been recent sporadic reports of brain metastases from breast cancer responding for some years to antiestrogens (particularly tamoxifen) or bromocriptine. We report three cases of brain metastasis from cancer--two multiple and one a solitary lesion.

Ribosomal protein S6 kinase and protein kinase C activation by epidermal growth factor after temporary renal ischemia.

An initial event in cell response to growth factors is the change in the state of phosphorylation of a number of cellular proteins playing a role in cell proliferation. The effects of a single dose of exogenously administered epidermal growth factor (EGF) on renal serine/threonine protein kinases such as ribosomal protein S6 kinase(s) and protein kinase C (PKC) and on [3H]thymidine incorporation into tubule cell nuclei have been studied during the regenerative repair response after temporary renal ischemia in the rat, followed by reperfusion for up to 72 h. During the postischemic reflow, the PKC and S6 kinase activities increased at 24 and 72 h, respectively.

Ribosomal protein S6 kinase is activated after folic acid injury and epidermal growth factor administration but not after unilateral nephrectomy in the rat kidney.

Following loss of functional renal mass induced by a single parenteral injection of folic acid, the increased proliferation of tubular epithelium to replace injured and necrotic cells was associated with S6 kinase activation and enhanced phosphorylation of this ribosomal protein in vitro, EGF administered 1.5 h after folic acid promoted the phosphorylation of S6 protein and accelerated the regenerative repair process. In contrast, the hypertrophic response of the kidney following uninephrectomy was not associated with S6 kinase activation.

Intraneural synovial cyst of the peroneal nerve: report of two cases and review of the literature.

We report two cases of peroneal nerve compression caused by an intraneural synovial cyst and discuss the pathogenesis, clinical and electrophysiological diagnosis, and treatment of these uncommon lesions in the light of the salient published work on the subject.

Primary intracranial lymphomas.

We present 20 cases of primary intracranial lymphoma and review the most important published series on this subject. The clinical patterns and the differential diagnosis from other intracranial space-occupying lesions on the CT and angiographic evidence are discussed. Surgical treatment was given in 19 of our cases, followed in 17 cases by radiotherapy and in 4 by chemotherapy.

Surgical treatment of subependymomas of the central nervous system. Report of 8 cases and review of the literature.

The authors present a series of 8 pure subependymomas of the central nervous system surgically treated between 1965 and 1987. The clinico-pathological features and the results of surgical treatment of these benign tumours are discussed in the light of the most important literature on this topic.

Intramedullary subependymoma of the cervical spinal cord.

The authors report a rare case of intramedullary subependymoma of the spinal cord. The case is discussed and a review of the literature is presented.

Mechanisms of regulation of ferritin synthesis in rat liver during experimental inflammation.

Liver slices of turpentine-treated rats were incubated in vitro and used as a model to study synthesis and secretion of proteins during the acute-phase response. The synthesis and secretion of typical acute-phase proteins increased after treatment. Similarly, ferritin increased at 24-48 hr after treatment.

Constitutive and induced synthesis of heat shock proteins in transplantable hepatomas.

The synthesis of heat shock proteins (HSP) was studied in rat liver and in a series of transplantable Morris hepatomas with different growth rates, subjected to heat shock in vivo and in vitro. Different from the liver, hepatomas synthesized HSP constitutively, i.e.