Publications by authors named "Barbora Neuzilova"
The pathogenic fungus utilizes a cyclic ferrioxamine E (FOXE) siderophore to acquire iron from the host. Biomimetic FOXE analogues were labeled with gallium-68 for molecular imaging with PET. [Ga]Ga(III)-FOXE analogues were internalized in cells via Sit1.
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- Siderophores are small molecules produced by microbes that help them acquire iron, and when radiolabelled with gallium-68, they can be used for targeted imaging of infections via PET.
- The study focused on two siderophore isomers, ferrirubin (FR) and ferrirhodin (FRH), which showed different properties in terms of hydrophilicity, protein binding, and pharmacokinetics after being labelled with gallium-68.
- Both siderophores effectively targeted bacteria in a mouse model, demonstrating their potential for infection imaging, with FR exhibiting faster clearance and lower blood retention compared to FRH.
The influence of various hydroxyl radical scavengers such as methanol, ethanol and dimethyl sulfoxide on radiation sensitivity of prokaryotic cells (bacteria Escherichia coli) and eukaryotic cells (yeast Saccharomyces cerevisiae and V79 cells-Chinese hamster pulmonary fibroblasts) irradiated by 60Co gamma radiation was investigated. The dependence of radiation sensitivity on dose rate in range from 1.8 to 100 Gy h-1 was evaluated.
View Article and Find Full Text PDFETHANOL AS A MODIFIER OF RADIATION SENSITIVITY OF LIVING CELLS AGAINST UV-C RADIATION.
Radiat Prot Dosimetry
December 2019
The protection of Escherichia coli bacteria and the yeast Saccharomyces cerevisiae against UV-C radiation by ethanol was studied. It was found that the fraction of surviving cells increases with increasing ethanol concentration. The specific protection depends on the dose rate, concentration range of ethanol, and it is higher for yeast compared to the bacteria.
View Article and Find Full Text PDFPurpose: The purpose of the paper was to investigate the protective effect of some scavengers of OH radicals (hydroxyl radicals) on the radiation sensitivity of bacteria (in some cases also yeast) under normoxic (in air) or hypoxic (suboxic) conditions and to compare the obtained results with those published earlier for the yeast, all in a wide interval of irradiation conditions. Another aim was to investigate a possible impact of the reaction order of the reaction between the scavengers and the OH radicals on the protection of the cells.
Materials And Methods: In order to study the protective effect of OH scavengers we used various concentrations of methanol and potassium formate (in some cases also ethanol) in isotonic salt solutions.