NAC regulates metabolism and cell fate in intestinal stem cells.

Intestinal stem cells (ISCs) face the challenge of integrating metabolic demands with unique regenerative functions. Studies have shown an intricate interplay between metabolism and stem cell capacity; however, it is still not understood how this process is regulated. Combining ribosome profiling and CRISPR screening in intestinal organoids, we identify the nascent polypeptide-associated complex (NAC) as a key mediator of this process.

Integrating social media-based community of inquiry with theory of planned behavior to promote equitable educational intentions among pre-service teachers in Gilgit Baltistan, Pakistan.

An equitable education system is essential for all students to acquire the knowledge and skills necessary to become productive members of society. Pre-service teachers in education play a vital role in fostering equitable educational practices. This study aimed to measure the association between the social media-based community of inquiry and pre-service teachers' intentions toward social justice and equity in education.

Clinical, microbiological and immunological short, medium and long-term effects of different strains of probiotics as an adjunct to non-surgical periodontal therapy in patients with periodontitis. Systematic review with meta-analysis.

Introduction/objectives: Probiotics have been proposed as adjuncts to non-surgical periodontal therapy (NSPT), however, the effect of their use remains unclear. The aim of this systematic review and meta-analysis was to analyze the evidence regarding the use of probiotics as an adjunct to NSPT in patients with periodontitis at a clinical, microbiological and immunological level.

Data/sources: A comprehensive search to identify clinical studies investigating the use of probiotics as an adjunct to NSPT in patients treated for periodontitis was performed.

Daratumumab triplet therapies in patients with relapsed or refractory multiple myeloma: A "real world" experience.

We report our retrospective analysis on 34 relapsed/refractory multiple myeloma (RRMM) patients treated with daratumumab based triplets. Twenty patients were females and 14 males. Median age was 73.

Azacitidine to Consolidate and Deepen the Therapeutic Response Achieved by Intensive Induction Treatment in a Young Patient Affected by NPM1mut-AML Who Has Become Ineligible for High-Dose Consolidation.

Acute myeloid leukemia (AML) is the most common leukemia in adults. In spite of the most recent discoveries about the molecular landscape of this disease, the treatment of elderly and unfit young patients continues to be a great challenge. The hypomethylating agents (HMA) still represent an effective therapeutic option for these categories, especially for the low-risk subgroups.

Support through Social Media and Online Class Participation to Enhance Psychological Resilience.

Social support was an important factor in minimizing the effect of social isolation during the COVID-19 pandemic lockdown. This research aimed to study the role of online class participation and social media usage to link the social support available from family and friends to psychological resilience among pre-service special education teachers against the negative psychological effects of the COVID-19 pandemic lockdown. A survey was conducted with 377 pre-service special needs education (SNE) teachers enrolled at universities in Pakistan.

Breaching Learners' Social Distancing through Social Media during the COVID-19 Pandemic.

Higher education has been shifted toward blended learning during the COVID-19 pandemic. An increase in social media usage intensity and reduced face-to-face interaction due to the COVID-19 pandemic urged instructional communication researchers to revisit the dynamics of learners' group development in terms of their socialization and academic performance during the COVID-19 crisis. This research aimed to determine the mediating role of social media sociability between face-to-face socialization and academic performance of higher education students in blended learning environments during the COVID-19 pandemic.

A prognostic model for patients with lymphoma and COVID-19: a multicentre cohort study.

Lymphoma represents a heterogeneous hematological malignancy (HM), which is characterized by severe immunosuppression. Patients diagnosed of coronavirus disease 2019 (COVID-19) during the course of HM have been described to have poor outcome, with only few reports specifically addressing lymphoma patients. Here, we investigated the clinical behavior and clinical parameters of a large multicenter cohort of adult patients with different lymphoma subtypes, with the aim of identifying predictors of death.

Synthesis of Hydroxamic Acid Derivatives Using Blocked (Masked) -Isocyanate Precursors.

Hydroxamic acids are present in a several pharmaceuticals and agrochemicals. Synthetic strategies providing access to hydroxamic acid derivatives remain limited, typically requiring the use of nucleophilic hydroxylamine reagents. Herein, a synthesis of hydroxamates from unactivated carboxylic acids is reported making use of rare blocked (masked) -substituted isocyanates.

Modeling the oxygen inhibition in microalgae: An experimental approach based on photorespirometry.

Microalgae cultivation has been the object of relevant interest for many industrial applications. Where high purity of the biomass/product is required, closed photobioreactors (PBRs) appear to be the best technological solution. However, as well as cost, the major drawback of closed systems is oxygen accumulation, which is well known to be responsible for growth inhibition.

Isavuconazole Therapy of Disseminated and Encephalic Infection in an Acute Myeloid Leukemia Patient Treated with Midostaurin.

Background: is a rare and emerging opportunistic fungus, involving immunocompromised hosts, in particular, neutropenic patients after chemotherapy. We report a case of disseminated and cerebral infection by , in a 68-year-old woman affected by acute myeloid leukemia that was successfully managed with liposomal amphotericin B and isavuconazole.

Conclusions: This case illustrates the feasibility of isavuconazole therapy in the treatment of a infection when co-administered with midostaurin.

Indicators of liberation from gender-based intimate partner violence in Spain related to when charges are dropped.

Background: While the reasons female victims of gender-based intimate partner violence (GIPV) withdraw from legal proceedings continue to fuel debate, little is known about the heterogeneity of these women regarding the timing of their decision and its connection to liberating themselves from the violent relationship.

Method: We used Analyses of Variance and Chi-squared tests to identify variables related to withdrawal from legal proceedings and variables indicating progress in the separation process related to the timing of withdrawal: in the initial or final stages of the legal proceedings. The analyses were performed in a sample of 105 women who had dropped charges.

Continuous Cultivation as a Method to Assess the Maximum Specific Growth Rate of Photosynthetic Organisms.

Modeling the growth of photosynthetic organisms is challenging, due to the complex role of light, which can be limiting because of self-shading, or photoinhibiting in the case of high intensities. A case of particular interest is represented by nitrogen-fixing cyanobacteria, whose growth is controlled not only by the light intensity, but also by the availability of atmospheric nitrogen in the liquid medium. The determination of the maximum specific growth rate is often affected by many variables that, in batch growth systems, may change significantly.

Respirometry as a tool to quantify kinetic parameters of microalgal mixotrophic growth.

Modeling microalgal mixotrophy is challenging, as the regulation of algal metabolism is affected by many environmental factors. A reliable tool to simulate microalgal behavior in complex systems, such as wastewaters, may help in setting the proper values of operative variables, provided that model parameters have been properly evaluated. In this work, a new respirometric protocol is proposed to quickly obtain the half-saturation constant values for several nutrients.

Functional CRISPR screen identifies AP1-associated enhancer regulating FOXF1 to modulate oncogene-induced senescence.

Background: Functional characterization of non-coding elements in the human genome is a major genomic challenge and the maturation of genome-editing technologies is revolutionizing our ability to achieve this task. Oncogene-induced senescence, a cellular state of irreversible proliferation arrest that is enforced following excessive oncogenic activity, is a major barrier against cancer transformation; therefore, bypassing oncogene-induced senescence is a critical step in tumorigenesis. Here, we aim at further identification of enhancer elements that are required for the establishment of this state.

LncRNA-OIS1 regulates DPP4 activation to modulate senescence induced by RAS.

Oncogene-induced senescence (OIS), provoked in response to oncogenic activation, is considered an important tumor suppressor mechanism. Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nt without a protein-coding capacity. Functional studies showed that deregulated lncRNA expression promote tumorigenesis and metastasis and that lncRNAs may exhibit tumor-suppressive and oncogenic function.

Cultivation of Scenedesmus obliquus in liquid hydrolysate from flash hydrolysis for nutrient recycling.

The production of biofuels from microalgae is associated with high demands of nutrients (nitrogen and phosphorus) required for growth. Recycling nutrients from the residual biomass is essential to obtain a sustainable production. In this work, the aqueous phase obtained from flash hydrolysis of Scenedesmus sp.

Maximizing the production of Scenedesmus obliquus in photobioreactors under different irradiation regimes: experiments and modeling.

Maximizing biomass productivity and photosynthetic efficiency are key factors to develop large-scale microalgae cultivation for biodiesel production. If the photobioreactor (PBR) is not operated under proper conditions, productivity and efficiency values drop considerably. In this work, the growth of Scenedesmus obliquus in continuous flat-panel PBR is considered.

A two or three compartments hyperbolic reaction-diffusion model for the aquatic food chain.

Two hyperbolic reaction-diffusion models are built up in the framework of Extended Thermodynamics in order to describe the spatio-temporal interactions occurring in a two or three compartments aquatic food chain. The first model focuses on the dynamics between phytoplankton and zooplankton, whereas the second one accounts also for the nutrient. In these models, infections and influence of illumination on photosynthesis are neglected.

Spread of infectious diseases in a hyperbolic reaction-diffusion susceptible-infected-removed model.

A one-dimensional hyperbolic reaction-diffusion model of epidemics is developed to describe the dynamics of diseases spread occurring in an environment where three kinds of individuals mutually interact: the susceptibles, the infectives, and the removed. It is assumed that the disease is transmitted from the infected population to the susceptible one according to a nonlinear convex incidence rate. The model, based upon the framework of extended thermodynamics, removes the unphysical feature of instantaneous diffusive effects, which is typical of parabolic models.

[In vitro study of the antimicrobial properties of a silver ion-releasing polyurethane foam].

Introduction: The antimicrobial properties of a silver ion (Ag+)-releasing polyurethane foam were evaluated using different microorganisms. The diffusion of Ag+ from the medium, as well as any possible cytotoxicity on human cells, was also studied.

Material And Methods: Silver release from V.

Activity of maize transglutaminase overexpressed in Escherichia coli inclusion bodies: an alternative to protein refolding.

Transglutaminases (TGases) catalyze protein post-translational modification by ε-(γ-glutamyl) links and covalent polyamine conjugation. In plants, this enzyme is poorly characterized and only the maize plastidial TGase gene (tgz) has been cloned. The tgz gene (Patent WWO03102128) had been subcloned and overexpressed in Escherichia coli cells, and the recombinant protein (TGZp) was present mainly in inclusion bodies (IB) fraction.

Purification and in vitro refolding of maize chloroplast transglutaminase over-expressed in Escherichia coli.

In contrast to mammalian transglutaminases (TGs), plant members of the superfamily are poorly characterized. In order to produce pure and active TG for its functional and structural studies, variants of maize chloroplast transglutaminase (TGZ, Patent WWO03102128) were sub-cloned into a pET28 vector and overexpressed in Escherichia coli BL21 (DE3) cells. The recombinant proteins were present mainly as insoluble inclusion bodies.