Implementation of a dynamic intestinal gut-on-a-chip barrier model for transport studies of lipophilic dioxin congeners.

Novel microfluidic technologies allow the manufacture of organ-on-a-chip systems that hold great promise to adequately recapitulate the biophysical and functional complexity of organs found . In this study, a gut-on-a-chip model was developed aiming to study the potential cellular association and transport of food contaminants. Intestinal epithelial cells (Caco-2) were cultured on a porous polyester membrane that was tightly clamped between two glass slides to form two separate flow chambers.