Phenotypic changes in lymphocyte subpopulations in pediatric renal-transplant patients after T-cell depletion.

Background: T-cell depletion causes a novel homeostasis in lymphocyte subsets in adult transplant recipients. Little is known about long-term changes in pediatric patients.

Methods: Twenty-one pediatric renal-transplant patients (mean age 11.

Cell lineage assignment of cytogenetic findings in acute lymphoblastic leukemia using combined immunomagnetic cell separation and chromosome preparation.

In acute lymphoblastic leukemia (ALL) abnormal karyotypes frequently constitute a minor part of the dividing cells, and the origin of metaphases in normal diploid cases remains obscure. We used a combination of immunomagnetic cell separation and chromosome preparation (ICSCP) to focus on the metaphases of interest and to assign the chromosome findings to CD19+ or CD7+ leukemia cells.