Complete genome sequence of BAA-1094.

We report the complete genome of BAA-1094 obtained from the American Type Culture Collection. The genome has one circular chromosome (5.7 Mbp).

Understanding Autologous Spliceostatin Transcriptional Regulation to Derive Parts for Heterologous Expression in a Bacterial Host.

β-Proteobacteria are emerging sources of natural products. We are interested in developing sp. FERM BP-3421 into a synthetic biology chassis to facilitate natural product discovery.

Complete Genome Sequence of Soil Bacterium sp. Strain FERM BP-3421, a Producer of Spliceostatins.

Here, we report the complete genome sequence of sp. strain FERM BP-3421, a bacterium isolated previously from a soil sample in Japan. Strain FERM BP-3421 produces spliceostatins, which are splicing modulatory antitumor agents that advanced to preclinical development.

Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria.

Bacterial natural products (NPs) retain high value in discovery efforts for applications in medicine and agriculture. Burkholderia β-Proteobacteria are a promising source of NPs. In this review, we summarize the recently developed genetic manipulation techniques used to access silent/cryptic biosynthetic gene clusters from Burkholderia native producers.

Draft Genome Sequence of sp. Strain BA2021, Isolated as a Contaminant of a Laboratory-Cultivated Predatory Myxobacterium.

During laboratory cultivation of the myxobacterium strain Cb vi76, a reoccurring contaminant was isolated and sequenced. Comparative taxonomic analysis of the draft genome suggested the contaminant to be a novel species, currently designated sp. strain BA2021, from the genus , members of which are considered promising biocontrol agents.

Myxobacterial Response to Methyljasmonate Exposure Indicates Contribution to Plant Recruitment of Micropredators.

Chemical exchanges between plants and microbes within rhizobiomes are critical to the development of community structure. Volatile root exudates such as the phytohormone methyljasmonate (MeJA) contribute to various plant stress responses and have been implicated to play a role in the maintenance of microbial communities. Myxobacteria are competent predators of plant pathogens and are generally considered beneficial to rhizobiomes.

Survey of Biosynthetic Gene Clusters from Sequenced Myxobacteria Reveals Unexplored Biosynthetic Potential.

Coinciding with the increase in sequenced bacteria, mining of bacterial genomes for biosynthetic gene clusters (BGCs) has become a critical component of natural product discovery. The order Myxococcales, a reputable source of biologically active secondary metabolites, spans three suborders which all include natural product producing representatives. Utilizing the BiG-SCAPE-CORASON platform to generate a sequence similarity network that contains 994 BGCs from 36 sequenced myxobacteria deposited in the antiSMASH database, a total of 843 BGCs with lower than 75% similarity scores to characterized clusters within the MIBiG database are presented.

Draft Genome Sequence of sp. Strain Cb G35.

In an effort to explore myxobacterial natural product biosynthetic pathways, the draft genome sequence of sp. strain Cb G35 has been obtained. Analysis of the genome using antiSMASH predicts 49 natural product biosynthetic pathways.