Acoustic radiation force on a long cylinder, and potential sound transduction by tomato trichomes.

Acoustic transduction by plants has been proposed as a mechanism to enable just-in-time up-regulation of metabolically expensive defensive compounds. Although the mechanisms by which this "hearing" occurs are unknown, mechanosensation by elongated plant hair cells known as trichomes is suspected. To evaluate this possibility, we developed a theoretical model to evaluate the acoustic radiation force that an elongated cylinder can receive in response to sounds emitted by animals, including insect herbivores, and applied it to the long, cylindrical stem trichomes of the tomato plant Solanum lycopersicum.

Arabidopsis Leaf Trichomes as Acoustic Antennae.

The much studied plant Arabidopsis thaliana has been reported recently to react to the sounds of caterpillars of Pieris rapae chewing on its leaves by promoting synthesis of toxins that can deter herbivory. Identifying participating receptor cells-potential "ears"-of Arabidopsis is critical to understanding and harnessing this response. Motivated in part by other recent observations that Arabidopsis trichomes (hair cells) respond to mechanical stimuli such as pressing or brushing by initiating potential signaling factors in themselves and in the neighboring skirt of cells, we analyzed the vibrational responses of Arabidopsis trichomes to test the hypothesis that trichomes can respond acoustically to vibrations associated with feeding caterpillars.

The Arabidopsis trichome is an active mechanosensory switch.

Trichomes ('hair cells') on Arabidopsis thaliana stem and leaf surfaces provide a range of benefits arising from their shape and disposition. These include tempting herbivores to sample constitutive toxins before they reach the bulk of the tissue. We asked whether, in addition, small mechanical disturbances such as an insect can make elicit signals that might help the plant respond to herbivory.

Structures formed by a cell membrane-associated arabinogalactan-protein on graphite or mica alone and with Yariv phenylglycosides.

Background: Certain membrane-associated arabinogalactan-proteins (AGPs) with lysine-rich sub-domains participate in plant growth, development and resistance to stress. To complement fluorescence imaging of such molecules when tagged and introduced transgenically to the cell periphery and to extend the groundwork for assessing molecular structure, some behaviours of surface-spread AGPs were visualized at the nanometre scale in a simplified electrostatic environment.

Methods: Enhanced green fluorescent protein (EGFP)-labelled LeAGP1 was isolated from Arabidopsis thaliana leaves using antibody-coated magnetic beads, deposited on graphite or mica, and examined with atomic force microscopy (AFM).

A model for leaf initiation: determination of phyllotaxis by waves in the generative circle.

A biophysical model is proposed for how leaf primordia are positioned on the shoot apical: meristem in both spiral and whorl phyllotaxes. Primordia are initiated by signals that propagate: in the epidermis in both azimuthal directions away from the cotyledons or the most recently: specified primordia. The signals are linear waves as inferred from the spatial periodicity of the: divergence angle and a temporal periodicity.

"Second extrinsic organizational mechanism" for orienting cellulose: modeling a role for the plasmalemmal reticulum.

Oriented deposition of cellulose fibers by cellulose-synthesizing complexes typically occurs across the plasma membrane from microtubule bundles and is guided by them. However, aligned movement of the complexes can be shown even after applied oryzalin has depolymerized microtubules. Further, there is a claim that when (1) microtubules are depolymerized with oryzalin, (2) a microtubule-orienting stimulus is applied temporarily, and (3) oryzalin is washed out, the newly forming cellulose fibers are oriented with respect to the stimulus.

Ca pulsation in BY-2 cells and evidence for control of mechanosensory Ca-selective channels by the plasmalemmal reticulum.

A previously unknown cytoskeletal structure, now named the plasmalemmal reticulum (Gens et al. 2000, Protoplasma 212, 115-134), was found in cultured BY-2 tobacco cells during a search for a force-focusing mechanism that might enhance signal transduction by the cells' mechanosensory Ca-selective cation channels (MCaCs). This polyhedral structure, which links cell wall, plasma membrane, and internal cytoplasm, prominently contains arabinogalactan protein (AGP).

Control of gravitropic orientation. II. Dual receptor model for gravitropism.

Gravitropism of vascular plants has been assumed to require a single gravity receptor mechanism. However, based on the evidence in Part I of this study, we propose that maize roots require two. The first mechanism is without a directional effect and, by itself, cannot give rise to tropism.

Control of gravitropic orientation. I. Non-vertical orientation by primary roots of maize results from decay of competence for orthogravitropic induction.

Plant organs may respond to gravity by vertical (orthogravitropic), oblique (plagiogravitropic) or horizontal (diagravitropic) growth. Primary roots of maize (Zea mays L.) provide a good system for studying such behaviours because they are reportedly capable of displaying all three responses.

Subcellular targeting of nine calcium-dependent protein kinase isoforms from Arabidopsis.

Calcium-dependent protein kinases (CDPKs) are specific to plants and some protists. Their activation by calcium makes them important switches for the transduction of intracellular calcium signals. Here, we identify the subcellular targeting potentials for nine CDPK isoforms from Arabidopsis, as determined by expression of green fluorescent protein (GFP) fusions in transgenic plants.