First Records of Heartbeats via ECG in a Stingless Bee, (Apidae, Meliponini), during Contention Stress Using Isoflurane as an Anesthetic.

The hemodynamic activity of workers was evaluated during restraint stress for a period of 30 min. The observed parameters were power variation in the elapsed time, and subsequently, six periods of one second were divided and called A, B, C, D, E and F; in each period, the electrocardiographic parameters were evaluated: spike frequency, amplitude, spike intervals and spike duration. The experiment was carried out with eight worker bees of , for which electrodes of a nickel-chromium alloy were made.